First Report of Glyphosate-Resistant Biotype of Eleusine Indica (L.) Gaertn. in Europe

Loddo, Donato; Imperatore, Gaetano; Milani, Andrea; Panozzo, Silvia; Farinati, Silvia; Sattin, Maurizio; Zanin, Giuseppe

doi:10.3390/agronomy10111692

Open AccessArticle

First Report of Glyphosate-Resistant Biotype of Eleusine Indica (L.) Gaertn. in Europe

by

Donato Loddo

^1,*

,

Gaetano Imperatore

²,

Andrea Milani

¹

,

Silvia Panozzo

¹

,

Silvia Farinati

¹,

Maurizio Sattin

¹

and

Giuseppe Zanin

²

¹

Institute for Sustainable Plant Protection (IPSP-CNR), National Research Council of Italy, Viale dell’Università 16, 35020 Legnaro, Italy

²

Department of Agronomy, Food, Natural Resources, Animals and Environment, DAFNAE, University of Padua, Viale dell’Università 16, 35020 Legnaro, Italy

^*

Author to whom correspondence should be addressed.

Agronomy 2020, 10(11), 1692; https://doi.org/10.3390/agronomy10111692

Submission received: 9 October 2020 / Revised: 26 October 2020 / Accepted: 28 October 2020 / Published: 31 October 2020

(This article belongs to the Special Issue Integrated Weed Management Approaches and Decision Support Systems)

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Abstract

Glyphosate-resistant biotypes of Eleusine indica (L.) Gaertn. have been detected in Asia, the Americas but not in Europe. The aim of this study was to evaluate resistance levels and possible target site resistance mechanisms of an E. indica biotype (19-1) collected from a plant nursery in Southern Italy where poor glyphosate efficacy was reported. Two dose-response experiments were conducted to evaluate the sensitivity of biotype 19-1 to glyphosate in comparison with two susceptible checks. 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) sequencing was performed to identify possible mutations conferring the resistance. The susceptible biotypes were completely controlled at the glyphosate recommended field dose of 360 g ae ha⁻¹, while 50% of the plants of biotype 19-1 survived at 1440 g ae ha⁻¹. The resistance index of biotype 19-1 ranged between 5.8 and 7.3 for the response variables of fresh weight reduction and plant survival, respectively. All the plants surviving glyphosate application and sampled for DNA analyses had the point mutation P106A. The biotype 19-1 can be confirmed as glyphosate-resistant, representing the first glyphosate-resistant population of E. indica in Europe.

Keywords: goosegrass; glyphosate resistance; herbicide resistance; plant nursery

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MDPI and ACS Style

Loddo, D.; Imperatore, G.; Milani, A.; Panozzo, S.; Farinati, S.; Sattin, M.; Zanin, G. First Report of Glyphosate-Resistant Biotype of Eleusine Indica (L.) Gaertn. in Europe. Agronomy 2020, 10, 1692. https://doi.org/10.3390/agronomy10111692

AMA Style

Loddo D, Imperatore G, Milani A, Panozzo S, Farinati S, Sattin M, Zanin G. First Report of Glyphosate-Resistant Biotype of Eleusine Indica (L.) Gaertn. in Europe. Agronomy. 2020; 10(11):1692. https://doi.org/10.3390/agronomy10111692

Chicago/Turabian Style

Loddo, Donato, Gaetano Imperatore, Andrea Milani, Silvia Panozzo, Silvia Farinati, Maurizio Sattin, and Giuseppe Zanin. 2020. "First Report of Glyphosate-Resistant Biotype of Eleusine Indica (L.) Gaertn. in Europe" Agronomy 10, no. 11: 1692. https://doi.org/10.3390/agronomy10111692

APA Style

Loddo, D., Imperatore, G., Milani, A., Panozzo, S., Farinati, S., Sattin, M., & Zanin, G. (2020). First Report of Glyphosate-Resistant Biotype of Eleusine Indica (L.) Gaertn. in Europe. Agronomy, 10(11), 1692. https://doi.org/10.3390/agronomy10111692

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First Report of Glyphosate-Resistant Biotype of Eleusine Indica (L.) Gaertn. in Europe

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