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Article

Treatment of Sweet Pepper with Stress Tolerance-Inducing Compounds Alleviates Salinity Stress Oxidative Damage by Mediating the Physio-Biochemical Activities and Antioxidant Systems

by
Khaled A. Abdelaal
1,
Lamiaa M. EL-Maghraby
2,
Hosam Elansary
3,4,
Yaser M. Hafez
1,
Eid I. Ibrahim
5,
Mostafa El-Banna
6,
Mohamed El-Esawi
7,8 and
Amr Elkelish
9,*
1
Plant Pathology and Biotechnology Lab., Excellence Center (EPCRS), Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
2
Agricultural Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt
3
Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
4
Floriculture, Ornamental Horticulture and Garden Design Department, Faculty of Agriculture, Alexandria University, Alexandria 21526, Egypt
5
Rice Biotechnology Lab., Rice Research Dep., Field Crops Research Institute, Sakha, Kafr El-Sheikh 33717, ARC, Egypt
6
Agricultural Botany Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt
7
Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt
8
Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, UK
9
Botany Department, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt
*
Author to whom correspondence should be addressed.
Agronomy 2020, 10(1), 26; https://doi.org/10.3390/agronomy10010026
Submission received: 28 November 2019 / Revised: 12 December 2019 / Accepted: 19 December 2019 / Published: 23 December 2019
(This article belongs to the Special Issue Physiological and Molecular Characterization of Crop Resistance to Abiotic Stresses)
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Abstract

:
Salinity stress occurs due to the accumulation of high levels of salts in soil, which ultimately leads to the impairment of plant growth and crop loss. Stress tolerance-inducing compounds have a remarkable ability to improve growth and minimize the effects of salinity stress without negatively affecting the environment by controlling the physiological and molecular activities in plants. Two pot experiments were carried out in 2017 and 2018 to study the influence of salicylic acid (1 mM), yeast extract (6 g L−1), and proline (10 mM) on the physiological and biochemical parameters of sweet pepper plants under saline conditions (2000 and 4000 ppm). The results showed that salt stress led to decreasing the chlorophyll content, relative water content, and fruit yields, whereas electrolyte leakage, malondialdehyde (MDA), proline concentration, reactive oxygen species (ROS), and the activities of antioxidant enzymes increased in salt-stressed plants. The application of salicylic acid (1 mM), yeast extract (6 g L−1), and proline (10 mM) markedly improved the physiological characteristics and fruit yields of salt-stressed plants compared with untreated stressed plants. A significant reduction in electrolyte leakage, MDA, and ROS was also recorded for all treatments. In conclusion, our results reveal the important role of proline, SA, and yeast extracts in enhancing sweet pepper growth and tolerance to salinity stress via modulation of the physiological parameters and antioxidants machinery. Interestingly, proline proved to be the best treatment.

1. Introduction

Global food safety is seriously dependent on crops and their supplies, which require considerable increases for servicing the gap between production and demand [1]. The necessity of improving crop production has been much more emergent in the last few years due to the expanding population, which will exceed to 9.7 billion by 2050. Undoubtedly, increases in the population will exert pressure on crops and food resources [1]. Simultaneously, global warming, as well as various biotic and abiotic stresses, hinder the growth and yields of agricultural crops [2]. Among abiotic stresses, salinity is recognized as one of the main restricting factors affecting the growth and productivity of agricultural crops, especially in arid and semiarid regions [3]. Salinity stress causes a reduction in growth and biomass, chlorophyll degradation, water status modification, malfunctions in stomatal functions, modifications in transpiration and respiration, and disequilibria in ion ratios [4,5]. Furthermore, plants develop cytotoxic-activated oxygen under saline conditions, which might seriously interfere with healthy metabolisms as a result of the oxidative damage of lipids, proteins, and nucleic acids [6,7]. Salinization may additionally lead to the excessive intracellular generation of reactive oxygen species (ROS) such as hydroxyl radicals (OH) and superoxide radicals (O2−) [8]. Plants confront these sorts of oxidants by developing several defensive mechanisms, including antioxidant enzymes and molecules that eliminate potentially cytotoxic types of activated oxygen [9,10].
Sweet pepper (Capsicum annuum L.) is an important vegetable crop that is grown for local consumption, and which has a high economic value in the Egyptian agricultural market. Farmers started to utilize saline water to partially fulfil crop water demands. The pepper plant is not a salt-tolerant vegetable, and about 14% of fruit yield loss occurs as a result of each increase in salt level of 1.0 dS/m [11]. Previous investigations have been conducted to mitigate the harmful impact of salt stress on sweet pepper, but most have not been sufficient or broadly applicable. As a result, the search for cheaper, ecologically-friendly strategies for salinity amelioration which enhance the growth and productivity of sweet pepper has been very important to the agriculture sector [12].
Numerous studies have found that implementing exogenous chemicals improves salt stress tolerance in plants [13]; examples of such chemicals are phytohormones such as salicylic acid, sterols, and methyl jasmonate [2,14]. Other chemicals such as polyamines, melatonin, and sodium nitroprusside have also been used to enhance the tolerance of various crop plants to saline conditions [15].
Salicylic acid is an essential phenolic compound that regulates plant growth processes and responses to different environmental factors [16]. It is a stress tolerance inducer and an important signal in many physiological processes, such as proline metabolism and photosynthesis. It reduces oxidative stress in plants under environmental stress and enhances plant growth and productivity under salt- [17] and drought-stress conditions [18]. Foliar application of SA-enhanced growth characteristics of sweet pepper plants [6] has increased the chlorophyll concentrations and enzyme activitives in barley plants, as well as counteracting the deleterious impacts of salinity on faba beans [19]. Yeast extracts are the main source of various important compounds, such as amino acids, phytohormones, and vitamins [20,21]. The use of active yeast extracts has been shown to decrease the damaging impact of drought conditions on pea plants, and enhanced the growth performance and yield of stressed plants [22]. Yeast extract applications have led to improvements in the growth characteristics of bean and corn plants, such as the dry weight of leaves, the leaf area, and the number of leaves under drought conditions [23]. The application of yeast and NPK fertilizers has significantly enhanced chlorophyll concentrations and root yields in sugar beet plants [22]. Seaweed extracts have also improved plant tolerance to abiotic stresses. For example, the application of Ecklonia maxima seaweed extract has been shown to enhance the tolerance of zucchini squash plants to salinity stress by improving plant performance, shoot biomass yield, fruit quality, leaf gas exchange rate, SPAD index, and leaf nutritional status under saline conditions [24]. Furthermore, proline has a positive impact on the activity of enzymes and osmotic adjustment under stress conditions, while protecting enzyme denaturation and modulating osmoregulation [25]. The application of proline-modulated antioxidant enzymes such as peroxidase (POX) and catalase (CAT) in tobacco plants under salinity conditions plays a significant role in protein synthesis and accumulation in plants under stress conditions like drought and salinity in order to enhance the growth characteristics and yield [26,27,28,29,30].
Considering the variable effectiveness levels of salicylic acid, proline, and yeast extract on plants, as well as the harmful impact of salinity stress on the growth and productivity of important crops, the present study aims to evaluate and compare the levels of effectiveness of the three stress tolerance inducers, i.e., salicylic acid (1 mM), yeast extract (6 g L−1), and proline (10 mM), on the growth characteristics, antioxidants, physiological and biochemical parameters, and yield of sweet pepper plants (Capsicum annuum L.) grown under the same saline conditions in order to determine which stress tolerance inducer should be recommended for further enhancements of crop performance and tolerance.

2. Materials and Methods

2.1. Experiments Design and Treatments

Pot experiments were performed at Agricultural Botany Department, Faculty of Agriculture, Kafrelsheikh University, Egypt during the growing seasons of 2017 and 2018. Laboratory analyses were carried out at the Plant Pathology & Biotechnology Lab, and the EPECRS Excellence Center Kafrelsheikh University, Egypt. This research was conducted to study the impacts of salicylic acid (1 mM), yeast extract (6 g L−1), and proline (10 mM) on the growth characteristics and biochemical and yield parameters of salt-stressed sweet pepper plants (Capsicum annuum L.). Irrigation water was artificially salinized by applying NaCl at concentrations of 2000 and 4000 ppm. The seeds of sweet pepper cv. California Wonder were obtained from Sun Seed Company in USA. Ten seeds were sown in the nursery using foam trays. Forty-two days after sowing, seedlings were transplanted into pots (30 cm diameter); each pot contained 8 kg soil and 2 plants. The physical and chemical soil characteristics were recorded, according to the methods described by Abdelaal et al. [21], as follows. pH: 8.2; N: 32.4 ppm; P: 10.5 ppm; K: 289 ppm; electrical conductivity: 1.8 dS m−1, soil organic matter: 1.9%; sand: 17.3%; silt: 35.5%; and clay: 47.2%. Fertilizers were added in two equal doses as recommended (NPK, 135:40:35 kg/ha), plus essential micronutrients, whereas the first dose was added 15 days after transplanting and the second at the beginning of flowering stage [31]. The plants were treated twice (20 and 40 days after transplanting) with salicylic acid (1 mM), yeast (6 g L−1) and proline (10 mM). The experiment was done in a completely randomized design with five replicates (five pots with two plants each), and the following measurements were recorded after collecting the plant samples.

2.2. Physiological and Biochemical Analysis

For physiological and biochemical analyses, the samples were collected at 90 days after transplantation for use in the following assays.

2.2.1. Chlorophyll a and b Determination

For chlorophyll a and b determination, 5 mL N-N Dimethyl formamid was added to 1 g sweet pepper fresh leaves and placed in a refrigerator for 24 h. Following the centrifugation at 4000 g for 15 min, the optical density was calculated using spectrophotometer at 647 and 664 nm, according to Moran [32].

2.2.2. Calculation of Leaves Relative Water Content (RWC %) and Electrolyte Leakage (EL %)

The relative water content (RWC) in leaves was recorded according to the formula of Sanchez et al. [33] as follows: RWC = (FW − DW)/ (TW − DW) × 100, where FW is fresh weight, DW is dry weight, and TW is turgid weight. Electrolyte leakage (EL %) was estimated using the formula of Dionisio-Sese and Tobita [34] as follows: EL (%) = Initial electrical conductivity/final electrical conductivity × 100.

2.2.3. Proline Content Determination

Proline was assayed according to the method described by Bates et al. [35] with minor modifications. In brief, a plant sample (0.6 g) was extracted in sulfosalicylic acid (5%) followed by centrifugation at 10000 g for 7 min. The supernatants were diluted with water, mixed with 2% ninhydrin, heated at 94 °C for 30 min, and then cooled. Toluene was then added to the mixture, and the upper aqueous phase was spectrophotometrically assayed at 520 nm.

2.2.4. Calculation of Lipid Peroxidation and Reactive Oxygen Species (Superoxide and Hydrogen Peroxide)

The lipid peroxidation as malondialdehyde (MDA) in plant samples was calculated according to the method described by Heath and Packer [36] with minor modifications. In brief, 0.6 g of plant sample was extracted in TCA (0.1%), followed by centrifugation at 13,000 g for 8 min. The supernatants were mixed with thiobarbituric acid (0.5%) and TCA, and heated at 92 °C for 35 min, followed by cooling and centrifugation at 12,000 g for 8 min. Next, the supernatants’ absorbance was measured at 532 and 660 nm. Superoxide and hydrogen peroxide levels were also determined according to the method described by Badiani et al. [37].

2.2.5. Antioxidant Enzymes Activity (CAT and POX)

Plant samples (1.5 g) were extracted in Tris-HCl (100 mM, pH 7.5) containing Dithiothreitol (5 mM), MgCl2 (10 mM), EDTA (1 mM), magnesium acetate (5 mm), PVP-40 (1.6%), aphenylmethanesulfonyl fluoride (1 mM), and aproptinin (1 μg mL−1). The mixed solutions were filtered and centrifuged for 8 min at 13,000 rpm. The supernatants were utilized to record enzymes activities. The activity of CAT and POX of leafy samples was determined according to the method described by Aebi [38] and Hammerschmidt et al. [39]. The supernatant absorbance was shown spectrophotometrically to be 470 nm.

2.2.6. Fruit yields

At 120 days after transplanting, the number of fruits per plant, the fruit fresh weight per plant (g), and the total fruit yield (ton hectare−1) were recorded.

2.3. Statistical Analysis

Data represent the mean ± SD (standard deviation). Two-way analysis of variance was performed using SPSS ver. 19 (SPSS Inc., Chicago, IL, USA). A Tukey’s test was also carried out to determine whether a significant difference (p < 0.05) existed between mean values.

3. Results

3.1. Chlorophyll a and b Concentrations

According to our results in Figure 1, the concentrations of chlorophyll a and b were significantly decreased in sweet pepper plants under salt-stress conditions; the lowest values were recorded with 4000 ppm compared with 2000 ppm and control plants in the two growing seasons. However, the salt stressed plants treated with salicylic acid, yeast extract, and proline showed significant increases in chlorophyll a and chlorophyll b concentrations compared with stressed untreated plants in both seasons. Under salt stresses of 2000 and 4000 ppm, the maximum concentrations of chlorophyll a and b were recorded with proline treatment in both seasons.

3.2. Relative Water Content (RWC %)

Data obtained in Figure 1 showed that RWC decreased considerably in salt stressed plants; the greatest reduction was recorded in the plants exposed to salinity at 4000 ppm compared with control plants. The exogenous application of salicylic acid (1 mM), yeast (6 g L−1), and proline (10 mM) caused a significant increase in RWC in salt stressed plants (2000 and 4000 ppm) compared with salt stressed untreated plants. Furthermore, the best treatments under salinity of 2000 ppm were salicylic acid and proline. Under salt treatment at 4000 ppm, the application of yeast extract (6 g L−1) and proline (10 mM) showed the highest RWC in sweet pepper plants compared with SA treatment in stressed untreated plants in both seasons.

3.3. Electrolyte Leakage (EL %)

It may be noted from Figure 2 that salt stress at 2000 and 4000 ppm caused a significant increase in electrolyte leakage (EL); the maximum increase was recorded with a salinity level of 4000 ppm in both seasons. Interestingly, electrolyte leakage was significantly decreased upon the foliar application of salicylic acid (1 mM), yeast extract (6 g L−1), and proline (10 mM) compared with control plants in both seasons. The best treatment was proline under a salt stress of 2000 ppm in both seasons (Figure 2).

3.4. Proline Concentration

It is evident that proline had markedly accumulated in sweet pepper plants; the highest concentration was recorded with a salinity at 4000 ppm in comparison to the control plants (Figure 2). Intriguingly, the application of salicylic acid, yeast extract, and proline resulted in enhanced proline concentration under all salinity levels; the greatest result was observed with proline (10 mM).

3.5. Lipid Peroxidation (MDA) and Reactive Oxygen Species (Superoxide and Hydrogen Peroxide).

The results showed that lipid peroxidation (i.e., malondialdehyde or MDA), superoxide, and hydrogen peroxide were significantly increased under salt conditions compared with control plants in both seasons (Figure 3). The maximum levels of MDA, superoxide, and hydrogen peroxide were recorded at a salinity level of 4000 ppm, followed by 2000 ppm, in both seasons. On the other hand, the application of salicylic acid, yeast extract, and proline significantly reduced MDA, O2−, and H2O2 concentrations under all salinity levels compared to the stressed untreated plants. The best results were obtained with SA and proline.

3.6. Antioxidant Enzymes Activity

Antioxidant enzyme activities (CAT and POX) were assayed. The data presented in Figure 4 shows that the plants exposed to salt stress (2000 and 4000 ppm) had higher CAT and POX activity compared with control plants in both seasons. On the other hand, applications of salicylic acid (1 mM), yeast extract (6 g L−1), and proline (10 mM) led to reductions in the activities of CAT and POX in the salt-stressed plants.

3.7. Number of Fruits per Plant, Fruit Fresh Weight, and Total Fruit Yield (Ton Hectare−1).

According to our findings in Figure 5, salt stress at 2000 and 4000 ppm caused significant decreases in fruit number per plant, fruit fresh weight, and the total fruit yield (ton hectare−1) in both seasons. The lowest values of these traits were recorded with salt stressed plants at 4000 ppm concentration, followed by 2000 ppm. Nevertheless, the exogenous application of salicylic acid, yeast, and proline significantly improved the number of fruits per plant, fruit fresh weight, and total fruit yield (ton hectare−1) in the stressed treated plants compared with stressed untreated plants.
Interestingly, SA and proline treatments gave the maximum values of the three studied characteristics at a salinity concentration of 2000 ppm in the two seasons (Figure 5). Under salinity stress of 4000 ppm, the best results of fruit number per plant, fruit fresh weight, and total fruit yield (ton hectare−1) were recorded with proline, followed by SA and yeast extract in both seasons.

4. Discussion

The exogenous application of salicylic acid, proline, and yeast extract previously exhibited variable effectiveness levels on plant performance and tolerance to the harmful impact of salinity stress; therefore, the present study assessed and compared the effectiveness levels of these stress tolerance inducers on the growth characteristics, antioxidant levels, physiological and biochemical parameters, and yield of sweet pepper plants (Capsicum annuum L.) grown under the same saline conditions in order to determine which stress tolerance inducer should be recommended for the enhancement of crop performance and tolerance. In the present study, salt stress significantly decreased the aforementioned physiological parameters of sweet pepper plants. This reduction in chlorophyll a and b concentrations could be due to the effect of salinity on chlorophyll-degrading enzyme (chlorophyllase) activity, which reduces the chlorophyll synthesis level or negatively affects the structure and number of chloroplasts [40,41,42]. The chloroplast is one of the most vital organelles for photosynthesis and plant production, and is dramatically affected by abiotic stresses [43,44]. The obtained results indicated that foliar applications of SA (1 mM), yeast extract (6 g L−1), and proline (10 mM) led to increased chlorophyll a and b contents in salt-stressed plants. These findings are in harmony with those obtained by Saleh et al. [25], Abdelaal et al. [21], and Soliman et al. [2]. These results might be due to the antioxidant scavenging influence of SA on chlorophyll degradation under saline conditions [45,46]. Correspondingly, the role of yeast extract in chlorophyll concentration enhancement might be due to the fact that yeast is rich in many essential elements, vitamins, and amino acids, which improve chlorophyll concentrations under stress conditions [21]. Moreover, our results showed that proline application minimized the harmful effects of all salinity levels on chlorophyll a and b concentrations due to its ability to function as a scavenger for ROS. Thus, proline plays a pivotal role in enzyme activation and protects chlorophyll from degradation under salt-stress conditions [47,48].
Additionally, relative water content (RWC) was decreased under salt stress. This decrease may be due to the reduction in water uptake [49] and/or its harmful effect on cell wall structure [50,51]. In contrast, RWC was significantly increased in stressed plants treated with SA, yeast, and proline. The ameliorative effects of these treatments on RWC could be due to the increase in osmoregulators, as well as to osmotic adjustment in plant cells [23,52,53].
Salt stress causes adverse effects on sweet pepper plants, including increased electrolyte leakage percentage. This increase may be due to the damaging effects on plasma membrane and selective permeability resulting in an increase in electrolyte leakage. This result is similar to that obtained in [54,55]. Conversely, the foliar application of SA, yeast, and proline led to decreased electrolyte leakage levels in all treatments. This beneficial effect could be due to the protective role of SA, yeast, and proline in plasma membrane stability and increasing soluble metabolite accumulation. A similar result was indicated by Ishikawa and Evans [56] and Huang et al. [57], who reported that osmoregulators improve plant growth and yield under various stress conditions.
Proline concentration was significantly increased in response to salt-stress conditions. This increment represents an important mechanism to minimize the deleterious impact of salinity stress and enhance plant growth [58]. The foliar application of SA, yeast, and proline under salt conditions may minimize the destructive effect of salinity on plant growth and improve proline accumulation. Similarly, SA application led to improved plant growth characteristics in maize plants under salt conditions [59]. Our results are in agreement with those of Huang et al. [60], Li et al. [61], and Gharsallah et al. [62]. Lipid peroxidation as MDA is an important factors indicating oxidative damage induced by salt stress. Lipid peroxidation was significantly boosted in salt-stressed (2000 and 4000 ppm) sweet pepper plants. Nonetheless, lipid peroxidation content was significantly decreased upon the foliar application of SA, yeast, and proline. These results may be attributed to the pivotal role of these treatments in decreasing oxidative stress damage, and consequently, in causing MDA reduction [25,48,53].
In the current study, superoxide and hydrogen peroxide, which are indicators of oxidative stress, were significantly produced in sweet pepper plants treated with NaCl at 2000 and 4000 ppm. This increase in superoxide and hydrogen peroxide production may be due to the fact that reactive oxygen species have a critical role under stress conditions in adjusting development, differentiation, redox levels, and stress signaling in the chloroplasts, mitochondria, and peroxisomes of plant cells [63,64]. Moreover, the high levels of hydrogen peroxide and superoxide are the main reasons for oxidative stress in the plant cells exposed to various stresses. Our results are supported by the findings of previous studies [65,66,67]. The application of SA, yeast, and proline on salt-stressed sweet pepper plants led to reductions in the formation of superoxide and hydrogen peroxide. This effect may be due to the role of these treatments in stabilizing protein structures and maintaining the redox states of plant cells, as well as stimulating antioxidant enzymes system [6,21,48]. Under salt stress (2000 and 4000 ppm), antioxidant enzyme activities were significantly increased in sweet pepper plants in order to combat the harmful impact of salt by adjusting osmotic balance. In agreement with our findings, similar results were noted in various plants under saline and drought conditions [68,69]. The activation of CAT and POX enzymes under salt conditions plays a key role in the improvement of plant defense systems. In the current study, the exogenous foliar application of SA, yeast, and proline led to improved antioxidant enzymes activity, as well as guarding the plant cells against oxidative stress and dehydration of the plasma membrane under salt-stress conditions. These results were supported by the findings reported in various plants [70,71,72].
The reductions of fruit numbers per plant, fruit fresh weight, and total fruit yield (ton hectare−1) under salt conditions are possibly due to the adverse impacts of salinity on the growth characteristics and physiological processes such as water uptake, photosynthesis, flowering, and fruit formation, which led to diminished yields. Accordingly, the highest level of salt (4000 ppm) was adversely more effective than the lowest one (2000 ppm). The same trends of salt stress were previously described in faba bean [73] and strawberry plants [74]. Our results indicate that proline treatment was the best, followed by SA and yeast treatments. This useful effect of proline may be due to its pivotal role in osmotic regulation, enzyme activation, and protein synthesis, which consequently enhances the growth and yield characteristics of stressed plants [47,75,76]. Also, SA plays an essential role as a stress tolerance inducer via reducing the oxidative damage and enhancing plant productivity under salt stress. These results are in harmony with previous findings of Gupta and Huang [77], Ahanger et al. [78], and Husen et al. [79].

5. Conclusions

According to our findings, salt stress caused significant decreases in chlorophyll concentrations, relative water content, and fruit yields. However, lipid peroxidation, proline, electrolyte leakage, and reactive oxygen species were increased. Based on the results, the foliar application of salicylic acid (1 mM), yeast extract (6 g L−1), and proline (10 mM) was an effective method by which to overcome the injurious effects of salt stress on sweet pepper plants. It may be concluded that relative water content and chlorophyll concentration, as well as antioxidant enzyme activity, were significantly modulated in the stressed treated sweet pepper plants. In contrast, electrolyte leakage and lipid peroxidation were decreased in treated sweet pepper plants under salt conditions. Thus, the application of salicylic acid, yeast, and proline led to a decrease in the harmful effects of salt stress by regulating osmolytes and antioxidants, which ultimately enhances the growth characteristics and fruit yields of sweet pepper plants. Interestingly, proline proved to be the best treatment for the further enhancement of plant performance and tolerance to salinity stress.

Author Contributions

K.A.A., L.M.E.-M., H.E., Y.M.H., E.I.I., M.E.-B., M.E.-E. and A.E. performed the experiments, analyzed the data, and wrote the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This work was funded by king Saud University funding through Researchers Supporting Project number (RSP-2019/118).

Acknowledgments

The authors extend their appreciation to king Saud University funding through Researchers Supporting Project number (RSP-2019/118) and also to all members of PPBL and EPCRS excellence center, Fac. of Agric., Kafrelsheikh University.

Conflicts of Interest

The authors have declared no conflict of interest.

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Figure 1. Effect of salinity stress (2000 and 4000 ppm NaCl) and supplementation of SA, yeast, and proline on the contents of (A) chlorophyll a, (B) chlorophyll b, (C) relative water content (RWC) in sweet pepper in the seasons of 2017 and 2018. Data is mean (±SE) of five replicates. Different letters in each Figure represent significant differences at p < 0.05.
Figure 1. Effect of salinity stress (2000 and 4000 ppm NaCl) and supplementation of SA, yeast, and proline on the contents of (A) chlorophyll a, (B) chlorophyll b, (C) relative water content (RWC) in sweet pepper in the seasons of 2017 and 2018. Data is mean (±SE) of five replicates. Different letters in each Figure represent significant differences at p < 0.05.
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Figure 2. Effect of salinity stress (2000 and 4000 ppm NaCl) and supplementation of SA, yeast, and proline on the contents of (A) electrolyte leakage, (B) proline in sweet pepper in the seasons of 2017 and 2018. Data is mean (±SE) of five replicates. Different letters in each Figure represent significant differences at p < 0.05.
Figure 2. Effect of salinity stress (2000 and 4000 ppm NaCl) and supplementation of SA, yeast, and proline on the contents of (A) electrolyte leakage, (B) proline in sweet pepper in the seasons of 2017 and 2018. Data is mean (±SE) of five replicates. Different letters in each Figure represent significant differences at p < 0.05.
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Figure 3. Effect of salinity stress (2000 and 4000 ppm NaCl) and supplementation of SA, yeast, and proline on the contents of (A) lipid peroxidation, (B) superoxide, (C) hydrogen peroxide in sweet pepper in the seasons of 2017 and 2018. Data is mean (±SE) of five replicates. Different letters in each Figure represent significant differences at p < 0.05.
Figure 3. Effect of salinity stress (2000 and 4000 ppm NaCl) and supplementation of SA, yeast, and proline on the contents of (A) lipid peroxidation, (B) superoxide, (C) hydrogen peroxide in sweet pepper in the seasons of 2017 and 2018. Data is mean (±SE) of five replicates. Different letters in each Figure represent significant differences at p < 0.05.
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Figure 4. Effect of salinity stress (2000 and 4000 ppm NaCl) and supplementation of SA, yeast, and proline on the activity of (A) Catalase (CAT), (B) peroxides (POX) in sweet pepper in the seasons of 2017 and 2018. Data is mean (±SE) of five replicates. Different letters in each Figure represent significant differences at p < 0.05.
Figure 4. Effect of salinity stress (2000 and 4000 ppm NaCl) and supplementation of SA, yeast, and proline on the activity of (A) Catalase (CAT), (B) peroxides (POX) in sweet pepper in the seasons of 2017 and 2018. Data is mean (±SE) of five replicates. Different letters in each Figure represent significant differences at p < 0.05.
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Figure 5. Effect of salinity stress (2000 and 4000 ppm NaCl) and supplementation of SA, yeast, and proline on (A) number of fruits plant−1, (B) fresh weight plant−1, (C) total fruit yield (ton hectare−1) in sweet pepper in the seasons of 2017 and 2018. Data is mean (±SE) of five replicates. Different letters in each Figure represent significant differences at p < 0.05.
Figure 5. Effect of salinity stress (2000 and 4000 ppm NaCl) and supplementation of SA, yeast, and proline on (A) number of fruits plant−1, (B) fresh weight plant−1, (C) total fruit yield (ton hectare−1) in sweet pepper in the seasons of 2017 and 2018. Data is mean (±SE) of five replicates. Different letters in each Figure represent significant differences at p < 0.05.
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Abdelaal, K.A.; EL-Maghraby, L.M.; Elansary, H.; Hafez, Y.M.; Ibrahim, E.I.; El-Banna, M.; El-Esawi, M.; Elkelish, A. Treatment of Sweet Pepper with Stress Tolerance-Inducing Compounds Alleviates Salinity Stress Oxidative Damage by Mediating the Physio-Biochemical Activities and Antioxidant Systems. Agronomy 2020, 10, 26. https://doi.org/10.3390/agronomy10010026

AMA Style

Abdelaal KA, EL-Maghraby LM, Elansary H, Hafez YM, Ibrahim EI, El-Banna M, El-Esawi M, Elkelish A. Treatment of Sweet Pepper with Stress Tolerance-Inducing Compounds Alleviates Salinity Stress Oxidative Damage by Mediating the Physio-Biochemical Activities and Antioxidant Systems. Agronomy. 2020; 10(1):26. https://doi.org/10.3390/agronomy10010026

Chicago/Turabian Style

Abdelaal, Khaled A., Lamiaa M. EL-Maghraby, Hosam Elansary, Yaser M. Hafez, Eid I. Ibrahim, Mostafa El-Banna, Mohamed El-Esawi, and Amr Elkelish. 2020. "Treatment of Sweet Pepper with Stress Tolerance-Inducing Compounds Alleviates Salinity Stress Oxidative Damage by Mediating the Physio-Biochemical Activities and Antioxidant Systems" Agronomy 10, no. 1: 26. https://doi.org/10.3390/agronomy10010026

APA Style

Abdelaal, K. A., EL-Maghraby, L. M., Elansary, H., Hafez, Y. M., Ibrahim, E. I., El-Banna, M., El-Esawi, M., & Elkelish, A. (2020). Treatment of Sweet Pepper with Stress Tolerance-Inducing Compounds Alleviates Salinity Stress Oxidative Damage by Mediating the Physio-Biochemical Activities and Antioxidant Systems. Agronomy, 10(1), 26. https://doi.org/10.3390/agronomy10010026

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