Next Article in Journal
Phospholipase D (PLD) Response to Water Stress in Citrus Roots and Leaves
Next Article in Special Issue
Comparison of Biochemical, Anatomical, Morphological, and Physiological Responses to Salinity Stress in Wheat and Barley Genotypes Deferring in Salinity Tolerance
Previous Article in Journal
Do Triticum aestivum L. and Triticum spelta L. Hybrids Constitute a Promising Source Material for Quality Breeding ofNew Wheat Varieties?
Previous Article in Special Issue
Treatment of Sweet Pepper with Stress Tolerance-Inducing Compounds Alleviates Salinity Stress Oxidative Damage by Mediating the Physio-Biochemical Activities and Antioxidant Systems
Open AccessArticle

Transcriptomic Profiling of Pomegranate Provides Insights into Salt Tolerance

by 1,2, 1,2, 1,2, 1,2, 3,* and 1,2,*
1
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
2
College of Forestry, Nanjing Forestry University, Nanjing 210037, China
3
Department of Horticultural Sciences, Texas A&M AgriLife Extension Service, College Station, TX 77843-2134, USA
*
Authors to whom correspondence should be addressed.
Agronomy 2020, 10(1), 44; https://doi.org/10.3390/agronomy10010044
Received: 17 October 2019 / Revised: 26 November 2019 / Accepted: 24 December 2019 / Published: 27 December 2019
Pomegranate (Punica granatum L.) is widely grown in arid and semi-arid soils, with constant soil salinization. To elucidate its molecular responses to salt stress on mRNA levels, we constructed 18 cDNA libraries of pomegranate roots and leaves from 0 (controls), 3, and 6 days after 200 mM NaCl treatment. In total, we obtained 34,047 genes by mapping to genome, and then identified 2255 DEGs (differentially expressed genes), including 1080 up-regulated and 1175 down-regulated genes. We found that the expression pattern of most DEGs were tissue-specific and time-specific. Among root DEGs, genes associated with cell wall organization and transmembrane transport were suppressed, and most of metabolism-related genes were over-represented. In leaves, 41.29% of DEGs were first suppressed and then recovered, including ions/metal ions binding-related genes. Also, ion transport and oxidation-reduction process were restricted. We found many DEGs involved in ABA, Ca2+-related and MAPK signal transduction pathways, such as ABA-receptors, Ca2+-sensors, MAPK cascades, TFs, and downstream functional genes coding for HSPs, LEAs, AQPs and PODs. Fifteen genes were selected to confirm the RNA-seq data using qRT-PCR. Our study not only illuminated pomegranate molecular responses to salinity, but also provided references for selecting salt-tolerant genes in pomegranate breeding processes. View Full-Text
Keywords: pomegranate; salt stress; transcriptome; tissue-specific; signaling transduction pathways; transcription factors pomegranate; salt stress; transcriptome; tissue-specific; signaling transduction pathways; transcription factors
Show Figures

Figure 1

MDPI and ACS Style

Liu, C.; Zhao, Y.; Zhao, X.; Wang, J.; Gu, M.; Yuan, Z. Transcriptomic Profiling of Pomegranate Provides Insights into Salt Tolerance. Agronomy 2020, 10, 44. https://doi.org/10.3390/agronomy10010044

AMA Style

Liu C, Zhao Y, Zhao X, Wang J, Gu M, Yuan Z. Transcriptomic Profiling of Pomegranate Provides Insights into Salt Tolerance. Agronomy. 2020; 10(1):44. https://doi.org/10.3390/agronomy10010044

Chicago/Turabian Style

Liu, Cuiyu; Zhao, Yujie; Zhao, Xueqing; Wang, Jinping; Gu, Mengmeng; Yuan, Zhaohe. 2020. "Transcriptomic Profiling of Pomegranate Provides Insights into Salt Tolerance" Agronomy 10, no. 1: 44. https://doi.org/10.3390/agronomy10010044

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop