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Open AccessArticle

The Development of Gelatin/Hyaluronate Copolymer Mixed with Calcium Sulfate, Hydroxyapatite, and Stromal-Cell-Derived Factor-1 for Bone Regeneration Enhancement

1
Department of Biomedical Engineering, National Taiwan University, No. 1, Sec.1, Jen-Ai Road, Taipei City 10051, Taiwan
2
Department of Orthopaedic Surgery, National Taiwan University Hospital, No. 7, Chung Shan South Road, Taipei City 10002, Taiwan
3
Integrative Stem Cell Center, China Medical University, No. 2, Yude Road, Taichung City 40447, Taiwan
*
Author to whom correspondence should be addressed.
Polymers 2019, 11(9), 1454; https://doi.org/10.3390/polym11091454
Received: 8 August 2019 / Revised: 24 August 2019 / Accepted: 2 September 2019 / Published: 5 September 2019
(This article belongs to the Special Issue Medical Application of Polymer-Based Composites)
In clinical practice, bone defects still remain a challenge. In recent years, apart from the osteoconductivity that most bone void fillers already provide, osteoinductivity has also been emphasized to promote bone healing. Stromal-cell-derived factor-1 (SDF-1) has been shown to have the ability to recruit mesenchymal stem cells (MSCs), which play an important role in the bone regeneration process. In this study, we developed a gelatin–hyaluronate (Gel-HA) copolymer mixed with calcium sulfate (CS), hydroxyapatite (HAP), and SDF-1 in order to enhance bone regeneration in a bone defect model. The composites were tested in vitro for biocompatibility and their ability to recruit MSCs after material characterization. For the in vivo test, a rat femoral condyle bone defect model was used. Micro computed tomography (Micro-CT), two-photon excitation microscopy, and histology analysis were performed to assess bone regeneration. As expected, enhanced bone regeneration was well observed in the group filled with Gel-HA/CS/HAP/SDF-1 composites compared with the control group in our animal model. Furthermore, detailed blood analysis of rats showed no obvious systemic toxicity or side effects after material implantation. In conclusion, the Gel-HA/CS/HAP/SDF-1 composite may be a safe and applicable material to enhance bone regeneration in bone defects. View Full-Text
Keywords: SDF-1; bone defect; bone regeneration; mesenchymal stem cells; gelatin; hyaluronate; calcium sulfate; hydroxyapatite; biomaterial SDF-1; bone defect; bone regeneration; mesenchymal stem cells; gelatin; hyaluronate; calcium sulfate; hydroxyapatite; biomaterial
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MDPI and ACS Style

Chang, Y.-L.; Hsieh, C.-Y.; Yeh, C.-Y.; Lin, F.-H. The Development of Gelatin/Hyaluronate Copolymer Mixed with Calcium Sulfate, Hydroxyapatite, and Stromal-Cell-Derived Factor-1 for Bone Regeneration Enhancement. Polymers 2019, 11, 1454.

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