Soy Protein-Based Composite Hydrogels: Physico-Chemical Characterization and In Vitro Cytocompatibility
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Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany
2
Section of Experimental Oncology and Nanomedicine, ENT-Department, University Hospital Erlangen University of Erlangen-Nuremberg, 91054 Erlangen, Germany
3
Department of Cardiology and Angiology, University Hospital Erlangen, University of Erlangen-Nuremberg, 91054 Erlangen, Germany
*
Authors to whom correspondence should be addressed.
Polymers 2018, 10(10), 1159; https://doi.org/10.3390/polym10101159
Received: 24 July 2018 / Revised: 10 October 2018 / Accepted: 12 October 2018 / Published: 17 October 2018
(This article belongs to the Special Issue Polymer Scaffolds for Biomedical Application)
Novel composite hydrogels based on the combination of alginate (Alg), soy protein isolate (SPI) and bioactive glass (BG) nanoparticles were developed for soft tissue engineering. Human umbilical vein endothelial cells (HUVEC) and normal human dermal fibroblasts were cultivated on hydrogels for 7, 14 and 21 days. Cell morphology was visualized using fluorescent staining at Days 7 and 14 for fibroblast cells and Days 14 and 21 for HUVEC. Metabolic activity of cells was analyzed using a colorimetric assay (water soluble tetrazolium (WST) assay). Compared to pure Alg, Alg/SPI and Alg/SPI/BG provided superior surfaces for both types of cells, supporting their attachment, growth, spreading and metabolic activity. Fibroblasts showed better colonization and growth on Alg/SPI/BG hydrogels compared to Alg/SPI hydrogels. The results indicate that such novel composite hydrogels might find applications in soft tissue regeneration.
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Keywords:
soy protein isolate; composite hydrogels; bioactive glass nanoparticles; biocompatibility; human cells; tissue engineering
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MDPI and ACS Style
Tansaz, S.; Singh, R.; Cicha, I.; Boccaccini, A.R. Soy Protein-Based Composite Hydrogels: Physico-Chemical Characterization and In Vitro Cytocompatibility. Polymers 2018, 10, 1159.
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