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Polymers 2018, 10(12), 1337; https://doi.org/10.3390/polym10121337

Laser Surface Microstructuring of a Bio-Resorbable Polymer to Anchor Stem Cells, Control Adipocyte Morphology, and Promote Osteogenesis

1
Ultraprecision Processes Unit, IK4-TEKNIKER, C/Iñaki Goenaga 5, 20600 Eibar, Spain
2
CIC bioGUNE, Technology Park of Bizkaia, Ed. 801A, 48160 Derio, Spain
3
Fraunhofer Institute for Integrated Systems and Device Technology IISB, Schottkystrasse 10, 91058 Erlangen, Germany
4
Institute for Biophysics, Department of Nanobiotechnology, University of Natural Resources and Life Sciences Vienna (BOKU), Muthgasse 11, 1190 Vienna, Austria
*
Author to whom correspondence should be addressed.
Received: 5 November 2018 / Revised: 29 November 2018 / Accepted: 30 November 2018 / Published: 3 December 2018
(This article belongs to the Special Issue Polymer Scaffolds for Biomedical Application)
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Abstract

New strategies in regenerative medicine include the implantation of stem cells cultured in bio-resorbable polymeric scaffolds to restore the tissue function and be absorbed by the body after wound healing. This requires the development of appropriate micro-technologies for manufacturing of functional scaffolds with controlled surface properties to induce a specific cell behavior. The present report focuses on the effect of substrate topography on the behavior of human mesenchymal stem cells (MSCs) before and after co-differentiation into adipocytes and osteoblasts. Picosecond laser micromachining technology (PLM) was applied on poly (L-lactide) (PLLA), to generate different microstructures (microgrooves and microcavities) for investigating cell shape, orientation, and MSCs co-differentiation. Under certain surface topographical conditions, MSCs modify their shape to anchor at specific groove locations. Upon MSCs differentiation, adipocytes respond to changes in substrate height and depth by adapting the intracellular distribution of their lipid vacuoles to the imposed physical constraints. In addition, topography alone seems to produce a modest, but significant, increase of stem cell differentiation to osteoblasts. These findings show that PLM can be applied as a high-efficient technology to directly and precisely manufacture 3D microstructures that guide cell shape, control adipocyte morphology, and induce osteogenesis without the need of specific biochemical functionalization. View Full-Text
Keywords: bio-resorbable polymers; picosecond laser micromachining technology; surface microstructuring; human mesenchymal stem cells; osteogenesis; adipocytes bio-resorbable polymers; picosecond laser micromachining technology; surface microstructuring; human mesenchymal stem cells; osteogenesis; adipocytes
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Ortiz, R.; Aurrekoetxea-Rodríguez, I.; Rommel, M.; Quintana, I.; Vivanco, M.D.; Toca-Herrera, J.L. Laser Surface Microstructuring of a Bio-Resorbable Polymer to Anchor Stem Cells, Control Adipocyte Morphology, and Promote Osteogenesis. Polymers 2018, 10, 1337.

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