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Catalysts 2018, 8(4), 170; https://doi.org/10.3390/catal8040170

Maltose Production Using Starch from Cassava Bagasse Catalyzed by Cross-Linked β-Amylase Aggregates

1
Graduate Program in Chemical Engineering, Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luiz, São Carlos 13565-905, Brazil
2
Kopp Technologies, Rua Alfredo Lopes, 1717, São Carlos 13560-460, Brazil
3
Departamento de Biocatálisis, ICP-CSIC, Campus UAM-CSIC Madrid, Madrid 28049, Spain
*
Authors to whom correspondence should be addressed.
Present address: Instituto de Engenharia de Várzea Grande, Universidade Federal de Mato Grosso, Av. Fernando Corrêa da Costa, 2367, Cuiabá 78060-900, Brazil.
Received: 20 March 2018 / Revised: 17 April 2018 / Accepted: 19 April 2018 / Published: 21 April 2018
(This article belongs to the Special Issue Immobilized Biocatalysts)
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Abstract

Barley β-amylase was immobilized using different techniques. The highest global yield was obtained using the cross-linked enzyme aggregates (CLEA) technique, employing bovine serum albumin (BSA) or soy protein isolate (SPI) as feeder proteins to reduce diffusion problems. The CLEAs produced using BSA or SPI showed 82.7 ± 5.8 and 53.3 ± 2.4% global yield, respectively, and a stabilization effect was observed upon immobilization at neutral pH value, e.g., after 12 h at 55 °C, the free β-amylase is fully inactivated, while CLEAs retained 25 and 15% of activity (using BSA and SPI, respectively). CLEA using SPI was selected because of its easier recovery, being chosen to convert the residual starch contained in cassava bagasse into maltose. This biocatalyst permitted to reach almost 70% of maltose conversion in 4 h using 30.0 g/L bagasse starch solution (Dextrose Equivalent of 15.88) and 1.2 U of biocatalyst per gram of starch at pH 7.0 and 40 °C. After 4 reuses (batches of 12 h) the CLEA using SPI maintained 25.50 ± 0.01% of conversion due to the difficulty of recovering. View Full-Text
Keywords: barley β-amylase; cross-linked enzyme aggregates (CLEA); cofeeders; enzyme immobilization; cassava bagasse barley β-amylase; cross-linked enzyme aggregates (CLEA); cofeeders; enzyme immobilization; cassava bagasse
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Araujo-Silva, R.; Mafra, A.C.O.; Rojas, M.J.; Kopp, W.; Giordano, R.D.C.; Fernandez-Lafuente, R.; Tardioli, P.W. Maltose Production Using Starch from Cassava Bagasse Catalyzed by Cross-Linked β-Amylase Aggregates. Catalysts 2018, 8, 170.

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