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Open AccessReview

Techniques for Preparation of Cross-Linked Enzyme Aggregates and Their Applications in Bioconversions

Liberal Arts Education Center, Tokai University, Kumamoto 862-8652, Japan
Department of Applied Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Hokkaido 060-8628, Japan
Advanced Manufacture Research Institute, National Institute of Advanced Industrial Science and Technology, Tosu, Saga 841-0052, Japan
Author to whom correspondence should be addressed.
Catalysts 2018, 8(5), 174;
Received: 27 March 2018 / Revised: 19 April 2018 / Accepted: 20 April 2018 / Published: 24 April 2018
(This article belongs to the Special Issue Immobilized Biocatalysts)
PDF [1252 KB, uploaded 3 May 2018]


Enzymes are biocatalysts. They are useful in environmentally friendly production processes and have high potential for industrial applications. However, because of problems with operational stability, cost, and catalytic efficiency, many enzymatic processes have limited applications. The use of cross-linked enzyme aggregates (CLEAs) has been introduced as an effective carrier-free immobilization method. This immobilization method is attractive because it is simple and robust, and unpurified enzymes can be used. Coimmobilization of different enzymes can be achieved. CLEAs generally show high catalytic activities, good storage and operational stabilities, and good reusability. In this review, we summarize techniques for the preparation of CLEAs for use as biocatalysts. Some important applications of these techniques in chemical synthesis and environmental applications are also included. CLEAs provide feasible and efficient techniques for improving the properties of immobilized enzymes for use in industrial applications. View Full-Text
Keywords: enzyme; immobilization; cross-linked enzyme aggregates enzyme; immobilization; cross-linked enzyme aggregates

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Yamaguchi, H.; Kiyota, Y.; Miyazaki, M. Techniques for Preparation of Cross-Linked Enzyme Aggregates and Their Applications in Bioconversions. Catalysts 2018, 8, 174.

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