A New Homo-Hexamer Mn-Containing Catalase from Geobacillus sp. WCH70
1
Key Laboratory for Molecular Enzymology & Engineering, the Ministry of Education, School of Life Sciences, Jilin University, 2699 Qianjin Street, Changchun 130012, Jilin, China
2
Department of Physics, Georgia Southern University, Statesboro, GA 30460, USA
*
Authors to whom correspondence should be addressed.
Catalysts 2017, 7(9), 277; https://doi.org/10.3390/catal7090277
Received: 2 August 2017
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Revised: 29 August 2017
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Accepted: 12 September 2017
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Published: 18 September 2017
(This article belongs to the Special Issue Biocatalysis and Biotransformations)
Catalase is an effective biocatalyst to degrade hydrogen peroxide to water and oxygen that can serve in textile effluent treatment to remove residual H2O2. Thermostable catalases are needed to withstand both the high temperature and pH of textile wastewater. We have cloned the Mn-containing catalase gene ACS24898.1 from Geobacillus sp. WCH70, which originated from thermophilic organisms, and expressed it in Escherichia coli in activated form. The recombinant protein has been purified to homogeneity and identified to be a new homo-hexamer Mn-containing catalase. The native molecular mass of the catalase has been measured to be 138 kDa by size-exclusion chromatography. The new enzyme has optimum catalyzed activity at pH 9.0 and a temperature of 75 °C. It is thermostable up to 70 °C for 8 h incubation and maintains 80% and 50% activity, respectively, at 80 °C after 5 h and 90 °C after 1 h. At 75 °C and pH 9.0, the Km is 67.26 mM for substrate H2O2 and the rate of reaction at H2O2 saturation, Vmax, is 75,300 U/mg. The thermophilic and alkaline preferred properties of this new Mn-catalase are valuable features in textile wastewater treatment.
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Keywords:
Mn-containing catalase; homo-hexamer; Geobacillus sp. WCH70; alkaline; thermophilic; textile wastewater
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MDPI and ACS Style
Li, H.-C.; Yu, Q.; Wang, H.; Cao, X.-Y.; Ma, L.; Li, Z.-Q. A New Homo-Hexamer Mn-Containing Catalase from Geobacillus sp. WCH70. Catalysts 2017, 7, 277. https://doi.org/10.3390/catal7090277
AMA Style
Li H-C, Yu Q, Wang H, Cao X-Y, Ma L, Li Z-Q. A New Homo-Hexamer Mn-Containing Catalase from Geobacillus sp. WCH70. Catalysts. 2017; 7(9):277. https://doi.org/10.3390/catal7090277
Chicago/Turabian StyleLi, Hai-Chao, Qing Yu, Hui Wang, Xin-Yu Cao, Li Ma, and Zheng-Qiang Li. 2017. "A New Homo-Hexamer Mn-Containing Catalase from Geobacillus sp. WCH70" Catalysts 7, no. 9: 277. https://doi.org/10.3390/catal7090277
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