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Article

Development of a Novel Bi-Enzymatic Nanobiocatalyst for the Efficient Bioconversion of Oleuropein to Hydroxytyrosol

by
Archontoula Giannakopoulou
1,
Alexandra V. Chatzikonstantinou
1,
Nikolaos Chalmpes
2,
Georgia Tsapara
1,
Dimitrios Gournis
2,
Angeliki C. Polydera
1 and
Haralambos Stamatis
1,*
1
Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece
2
Department of Materials Science & Engineering, University of Ioannina, 45110 Ioannina, Greece
*
Author to whom correspondence should be addressed.
Catalysts 2021, 11(6), 749; https://doi.org/10.3390/catal11060749
Submission received: 29 May 2021 / Revised: 15 June 2021 / Accepted: 16 June 2021 / Published: 19 June 2021
(This article belongs to the Special Issue State of the Art and Future Trends in Nanostructured Biocatalysis)
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Abstract

Lipase A from Candida antarctica (CalA) and β-glucosidase from Thermotoga maritima (bgl) were covalently co-immobilized onto the surface of chitosan-coated magnetic nanoparticles (CS-MNPs). Several parameters regarding the co-immobilization procedure (glutaraldehyde concentration, incubation time, CS-MNPs to enzyme mass ratio and bgl to CalA mass ratio) were evaluated and optimized. The developed nanobiocatalyst was characterized by various spectroscopic techniques. Biochemical parameters such as kinetic constants and thermal stability were also evaluated. The nanobiocatalytic system revealed an increase in the Km constant followed by a decrease in Vmax value compared with the native enzymes, while a significant increase (>5-fold higher) of the thermal stability of the immobilized CalA, both in individual and in co-immobilized form, was observed after 24 h incubation at 60 °C. Finally, the nanobiocatalyst was efficiently applied for the bioconversion of oleuropein to hydroxytyrosol, one of the most powerful naturally derived antioxidants, and it could be recycled for up to 10 reaction cycles (240 h of constant operation) at 60 °C, retaining more than 50% of its initial activity.
Keywords: multienzyme co-immobilization; lipase A from Candida antarctica; β-glucosidase from Thermotoga maritima; chitosan-magnetic nanoparticles; nanobiocatalysis; oleuropein modification multienzyme co-immobilization; lipase A from Candida antarctica; β-glucosidase from Thermotoga maritima; chitosan-magnetic nanoparticles; nanobiocatalysis; oleuropein modification
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MDPI and ACS Style

Giannakopoulou, A.; Chatzikonstantinou, A.V.; Chalmpes, N.; Tsapara, G.; Gournis, D.; Polydera, A.C.; Stamatis, H. Development of a Novel Bi-Enzymatic Nanobiocatalyst for the Efficient Bioconversion of Oleuropein to Hydroxytyrosol. Catalysts 2021, 11, 749. https://doi.org/10.3390/catal11060749

AMA Style

Giannakopoulou A, Chatzikonstantinou AV, Chalmpes N, Tsapara G, Gournis D, Polydera AC, Stamatis H. Development of a Novel Bi-Enzymatic Nanobiocatalyst for the Efficient Bioconversion of Oleuropein to Hydroxytyrosol. Catalysts. 2021; 11(6):749. https://doi.org/10.3390/catal11060749

Chicago/Turabian Style

Giannakopoulou, Archontoula, Alexandra V. Chatzikonstantinou, Nikolaos Chalmpes, Georgia Tsapara, Dimitrios Gournis, Angeliki C. Polydera, and Haralambos Stamatis. 2021. "Development of a Novel Bi-Enzymatic Nanobiocatalyst for the Efficient Bioconversion of Oleuropein to Hydroxytyrosol" Catalysts 11, no. 6: 749. https://doi.org/10.3390/catal11060749

APA Style

Giannakopoulou, A., Chatzikonstantinou, A. V., Chalmpes, N., Tsapara, G., Gournis, D., Polydera, A. C., & Stamatis, H. (2021). Development of a Novel Bi-Enzymatic Nanobiocatalyst for the Efficient Bioconversion of Oleuropein to Hydroxytyrosol. Catalysts, 11(6), 749. https://doi.org/10.3390/catal11060749

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