High-Resolution Quantitative Reconstruction of Microvascular Architectures in Mouse Hepatocellular Carcinoma Models
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animal Sample Preparation
2.2. Imaging
2.3. Image Preprocessing and Vessel Reconstruction
2.4. Histology and Immunohistochemistry
2.5. Statistical Data Analysis
3. Results
3.1. Validation of the Gelatin-FTIC Perfusion Method for Liver Vasculature Acquisition
3.2. Histopathology Analysis
3.3. Three-Dimensional Morphological Features of the Microvascular Systems
3.4. Cirrhosis and Tumors Specifically Affected the Sinusoidal Network Zone
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
CT | computed tomography |
FITC | fluorescein isothiocyanate |
H&E | hematoxylin and eosin |
HA | hepatic artery |
HCC | hepatocellular carcinoma |
HD-fMOST | high-definition fluorescence micro-optical sectioning tomography |
HV | hepatic vein |
LYVE1 | lymphatic endothelial receptor-1 |
PBS | phosphate-buffered saline |
PI | propidium iodide |
PV | portal vein |
TACE | transarterial chemoembolization |
WT | wild-type |
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Zhao, Y.; Zhao, H.; Wang, X.; Dai, W.; Ren, X.; Wang, J.; Cai, G. High-Resolution Quantitative Reconstruction of Microvascular Architectures in Mouse Hepatocellular Carcinoma Models. Cancers 2025, 17, 2653. https://doi.org/10.3390/cancers17162653
Zhao Y, Zhao H, Wang X, Dai W, Ren X, Wang J, Cai G. High-Resolution Quantitative Reconstruction of Microvascular Architectures in Mouse Hepatocellular Carcinoma Models. Cancers. 2025; 17(16):2653. https://doi.org/10.3390/cancers17162653
Chicago/Turabian StyleZhao, Yan, Haogang Zhao, Xin Wang, Wei Dai, Xuhua Ren, Jing Wang, and Guohong Cai. 2025. "High-Resolution Quantitative Reconstruction of Microvascular Architectures in Mouse Hepatocellular Carcinoma Models" Cancers 17, no. 16: 2653. https://doi.org/10.3390/cancers17162653
APA StyleZhao, Y., Zhao, H., Wang, X., Dai, W., Ren, X., Wang, J., & Cai, G. (2025). High-Resolution Quantitative Reconstruction of Microvascular Architectures in Mouse Hepatocellular Carcinoma Models. Cancers, 17(16), 2653. https://doi.org/10.3390/cancers17162653