LINC00963 Promotes Cancer Stemness, Metastasis, and Drug Resistance in Head and Neck Carcinomas via ABCB5 Regulation

Lee, Shiao-Pieng; Hsieh, Pei-Ling; Fang, Chih-Yuan; Chu, Pei-Ming; Liao, Yi-Wen; Yu, Chuan-Hang; Yu, Cheng-Chia; Tsai, Lo-Lin

doi:10.3390/cancers12051073

Submit to this Journal Review for this Journal Edit a Special Issue

Article Menu

...

Open AccessArticle

LINC00963 Promotes Cancer Stemness, Metastasis, and Drug Resistance in Head and Neck Carcinomas via ABCB5 Regulation

^1,2,†

^3,†,

^4,5,

³,

^6,7,8,

^6,7,8,

^6,7,8,* and

^4,5,*

School of Dentistry, National Defense Medical Center, Taipei 11490, Taiwan

Department of Dentistry, Tri-Service General Hospital, Taipei 11490, Taiwan

Department of Anatomy, School of Medicine, China Medical University, Taichung 404, Taiwan

⁴

School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan

⁵

Division of Oral and Maxillofacial Surgery, Department of Dentistry, Wan Fang Hospital, Taipei Medical University, Taipei 116, Taiwan

⁶

School of Dentistry, Chung Shan Medical University, Taichung 40201, Taiwan

⁷

Department of Dentistry, Chung Shan Medical University Hospital, Taichung 40201, Taiwan

⁸

Institute of Oral Sciences, Chung Shan Medical University, Taichung 40201, Taiwan

Authors to whom correspondence should be addressed.

^†

Equal contribution for the first authorship.

Cancers 2020, 12(5), 1073; https://doi.org/10.3390/cancers12051073

Received: 14 February 2020 / Revised: 10 April 2020 / Accepted: 20 April 2020 / Published: 26 April 2020

(This article belongs to the Special Issue Non-Coding RNAs as Emerging Regulators of Signalling Pathways and Novel Therapeutic Targets in Human Cancers)

View Full-Text Download PDF

Browse Figures

Citation Export

Abstract

Accumulating studies have indicated that long non-coding RNAs (lncRNAs) participate in the regulation of cancer stem cells (CSCs), which are crucial in tumor initiation, metastasis, relapse, and therapy resistance. In the current study, RT-PCR analysis was employed to evaluate the expression of LINC00963 in tumor tissues and oral CSCs. Stemness phenotypes and the expression of CSCs markers in oral cancer cells transfected with sh-LINC00963 were examined. Our results showed that the expression of the lncRNA LINC00963 was up-regulated in oral cancer tissues and CSCs. We found that the downregulation of LINC00963 inhibited CSC hallmarks, such as migration, invasion and colony formation capacity. Moreover, suppression of LINC00963 reduced the activity of stemness marker ALDH1, the percentage of self-renewal, chemoresistance and the expression of multidrug-resistance transporter ABCB5. Most importantly, we demonstrated that knockdown of LINC00963 decreased self-renewal, invasion and colony formation ability via ABCB5. Analysis of TCGA (the Cancer Genome Atlas) datasets suggested that the level of LINC00963 was positively correlated with the expression of the cancer stemness markers (Sox2 and CD44) and drug resistance markers (ABCG2 and ABCB5). Altogether, our results showed that suppression of LINC00963 may be beneficial to inhibit chemoresistance and cancer relapse in oral cancer patients. View Full-Text

Keywords: head and neck; lncRNA; LINC00963; cancer stemness; chemoresistance; ABCB5

▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Supplementary Material

Supplementary File 1:
PDF-Document (PDF, 249 KiB)

Share and Cite

MDPI and ACS Style

Lee, S.-P.; Hsieh, P.-L.; Fang, C.-Y.; Chu, P.-M.; Liao, Y.-W.; Yu, C.-H.; Yu, C.-C.; Tsai, L.-L. LINC00963 Promotes Cancer Stemness, Metastasis, and Drug Resistance in Head and Neck Carcinomas via ABCB5 Regulation. Cancers 2020, 12, 1073. https://doi.org/10.3390/cancers12051073

AMA Style

Lee S-P, Hsieh P-L, Fang C-Y, Chu P-M, Liao Y-W, Yu C-H, Yu C-C, Tsai L-L. LINC00963 Promotes Cancer Stemness, Metastasis, and Drug Resistance in Head and Neck Carcinomas via ABCB5 Regulation. Cancers. 2020; 12(5):1073. https://doi.org/10.3390/cancers12051073

Chicago/Turabian Style

Lee, Shiao-Pieng, Pei-Ling Hsieh, Chih-Yuan Fang, Pei-Ming Chu, Yi-Wen Liao, Chuan-Hang Yu, Cheng-Chia Yu, and Lo-Lin Tsai. 2020. "LINC00963 Promotes Cancer Stemness, Metastasis, and Drug Resistance in Head and Neck Carcinomas via ABCB5 Regulation" Cancers 12, no. 5: 1073. https://doi.org/10.3390/cancers12051073

Find Other Styles

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Abstract views Pdf views Html views

Article Menu

LINC00963 Promotes Cancer Stemness, Metastasis, and Drug Resistance in Head and Neck Carcinomas via ABCB5 Regulation

Abstract

Supplementary Material

Share and Cite

Article Metrics

Article Access Statistics

Article Access Map by Country/Region

Further Information

Guidelines

MDPI Initiatives

Follow MDPI