Sequential Colocalization of ERa, PR, and AR Hormone Receptors Using Confocal Microscopy Enables New Insights into Normal Breast and Prostate Tissue and Cancers
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. ColNu mIHCF Standardization
2.2. ColNu mIHCF in Normal Breast (NB) and Breast Cancer (BC)
2.3. ColNu mIHCF in Normal Prostate (NP) from Paired Cancer Cases: ERa Marks a p63 + Basal Cell Subpopulation
2.4. ColNu mIHCF in and Prostate Cancer (PC): Apical ERa is a Cancer Marker
2.5. Conventional IHC of ERa in Prostate Cancer in Tissue Sections and Needle-Core Biopsy
3. Discussion
4. Materials and Methods
4.1. Ethical Statement and Pathological and Clinical Data
4.2. Tissue Microarrays (TMA)
4.3. Prostate Needle-Core Biopsy
4.4. Immunohistochemistry (IHC)
4.5. ColNu mIHCF
4.6. Quantification of the Cell Populations
4.7. Graphs and Descriptive Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
TSA | Tyramide signal amplification |
TMA | Tissue microarray |
IVD | In vitro diagnostics |
ERa | Estrogen receptor alpha |
PR | Progesterone receptor |
AR | Androgen receptor |
NB | Normal breast |
BC | Breast cancer |
NP | Normal prostate |
PC | Prostate cancer |
DIC | Differential interfering contrast |
DAPI | 4′,6-Diamidino-2-phenylindole |
FFPE | Formalin-fixed paraffin-embedded |
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Chenlo, M.; Aliyev, E.; Rodrigues, J.S.; Vieiro-Balo, P.; Blanco Freire, M.N.; Cameselle-Teijeiro, J.M.; Alvarez, C.V. Sequential Colocalization of ERa, PR, and AR Hormone Receptors Using Confocal Microscopy Enables New Insights into Normal Breast and Prostate Tissue and Cancers. Cancers 2020, 12, 3591. https://doi.org/10.3390/cancers12123591
Chenlo M, Aliyev E, Rodrigues JS, Vieiro-Balo P, Blanco Freire MN, Cameselle-Teijeiro JM, Alvarez CV. Sequential Colocalization of ERa, PR, and AR Hormone Receptors Using Confocal Microscopy Enables New Insights into Normal Breast and Prostate Tissue and Cancers. Cancers. 2020; 12(12):3591. https://doi.org/10.3390/cancers12123591
Chicago/Turabian StyleChenlo, Miguel, Elvin Aliyev, Joana S. Rodrigues, Paula Vieiro-Balo, Manuel N. Blanco Freire, José Manuel Cameselle-Teijeiro, and Clara V. Alvarez. 2020. "Sequential Colocalization of ERa, PR, and AR Hormone Receptors Using Confocal Microscopy Enables New Insights into Normal Breast and Prostate Tissue and Cancers" Cancers 12, no. 12: 3591. https://doi.org/10.3390/cancers12123591