Next Article in Journal
Neurokinin-1 Receptor Antagonists against Hepatoblastoma
Previous Article in Journal
Baseline and Early Predictors of Good Patient Candidates for Second-Line after Sorafenib Treatment in Unresectable Hepatocellular Carcinoma
Article

PD-L1 Induction by Cancer-Associated Fibroblast-Derived Factors in Lung Adenocarcinoma Cells

1
Department of Anatomic Pathology, Tohoku University Graduate School of Medicine, Sendai, Miyagi 981-8575, Japan
2
Department of Disaster Obstetrics and Gynecology, International Research Institute of Disaster Science, Tohoku University, Sendai, Miyagi 980-8573, Japan
3
Division of Pathology, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi 981-8558, Japan
4
Department of Thoracic Surgery, Miyagi Cancer Centre, Natori, Miyagi 981-1293, Japan
5
Department of Pathology, Miyagi Cancer Centre, Natori, Miyagi 981-1293, Japan
6
Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Miyagi 981-8575, Japan
*
Author to whom correspondence should be addressed.
Cancers 2019, 11(9), 1257; https://doi.org/10.3390/cancers11091257
Received: 5 August 2019 / Accepted: 22 August 2019 / Published: 27 August 2019
Cancer-associated fibroblasts (CAFs) exert various effects upon biological behaviours of cancer. In this study, we examined the correlation of CAFs with the intra-tumoural immune system in the lung adenocarcinoma microenvironment. We studied 27 and 113 cases of lung adenocarcinoma tentatively as Cohorts 1 and 2, respectively. The patients in Cohort 1 received epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) for recurrent lung adenocarcinoma. α-smooth muscle actin (α-SMA), a surrogate marker for CAFs, was examined by immunohistochemistry. We then examined the effects of CAFs isolated from lung cancer tissues on programmed death ligand 1 (PD-L1) expression in lung adenocarcinoma cell lines. No significant associations were detected between α-SMA status and the ratios of CD8/CD4 and Foxp3/CD8 in Cohort 1. However, α-SMA status was significantly associated with PD-L1 status in both Cohorts 1 and 2. Conditioned medium of CAFs significantly induced PD-L1 expression in lung adenocarcinoma cell lines, A549, PC-9, and H1975. Among the cytokines examined by antibody array, C-X-C motif chemokine ligand 2 (CXCL2) increased PD-L1 mRNA expression in these cell lines. CXCL2 is therefore considered to have a potential to induce PD-L1 expression in lung adenocarcinoma cells as a result of an interaction between carcinoma cells and CAFs. These findings did firstly demonstrate that CAFs indirectly influenced tumour immunity through increasing PD-L1 expression in lung adenocarcinoma cells. View Full-Text
Keywords: cancer associated fibroblasts; tumor microenvironment; α-smooth muscle actin; programmed death ligand 1; lung adenocarcinoma cancer associated fibroblasts; tumor microenvironment; α-smooth muscle actin; programmed death ligand 1; lung adenocarcinoma
Show Figures

Figure 1

MDPI and ACS Style

Inoue, C.; Miki, Y.; Saito, R.; Hata, S.; Abe, J.; Sato, I.; Okada, Y.; Sasano, H. PD-L1 Induction by Cancer-Associated Fibroblast-Derived Factors in Lung Adenocarcinoma Cells. Cancers 2019, 11, 1257. https://doi.org/10.3390/cancers11091257

AMA Style

Inoue C, Miki Y, Saito R, Hata S, Abe J, Sato I, Okada Y, Sasano H. PD-L1 Induction by Cancer-Associated Fibroblast-Derived Factors in Lung Adenocarcinoma Cells. Cancers. 2019; 11(9):1257. https://doi.org/10.3390/cancers11091257

Chicago/Turabian Style

Inoue, Chihiro, Yasuhiro Miki, Ryoko Saito, Shuko Hata, Jiro Abe, Ikuro Sato, Yoshinori Okada, and Hironobu Sasano. 2019. "PD-L1 Induction by Cancer-Associated Fibroblast-Derived Factors in Lung Adenocarcinoma Cells" Cancers 11, no. 9: 1257. https://doi.org/10.3390/cancers11091257

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop