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Article

Rapid, Simple and Inexpensive Fabrication of Paper-Based Analytical Devices by Parafilm® Hot Pressing

1
Queensland Micro- and Nanotechnology Centre (QMNC), Griffith University, Nathan, QLD 4111, Australia
2
School of Engineering and Built Environment (EBE), Griffith University, Nathan, QLD 4111, Australia
3
School of Environment and Science (ESC), Griffith University, Nathan, QLD 4111, Australia
*
Author to whom correspondence should be addressed.
Academic Editors: Lung-Ming Fu and Mohammad Robiul Hossan
Micromachines 2022, 13(1), 48; https://doi.org/10.3390/mi13010048
Received: 3 December 2021 / Revised: 23 December 2021 / Accepted: 27 December 2021 / Published: 29 December 2021
(This article belongs to the Special Issue Feature Papers of Micromachines in Biology and Biomedicine 2021)
Paper-based analytical devices have been substantially developed in recent decades. Many fabrication techniques for paper-based analytical devices have been demonstrated and reported. Herein, we report a relatively rapid, simple, and inexpensive method for fabricating paper-based analytical devices using parafilm hot pressing. We studied and optimized the effect of the key fabrication parameters, namely pressure, temperature, and pressing time. We discerned the optimal conditions, including a pressure of 3.8 MPa, temperature of 80 °C, and 3 min of pressing time, with the smallest hydrophobic barrier size (821 µm) being governed by laminate mask and parafilm dispersal from pressure and heat. Physical and biochemical properties were evaluated to substantiate the paper functionality for analytical devices. The wicking speed in the fabricated paper strips was slightly lower than that of non-processed paper, resulting from a reduced paper pore size after hot pressing. A colorimetric immunological assay was performed to demonstrate the protein binding capacity of the paper-based device after exposure to pressure and heat from the fabrication. Moreover, mixing in a two-dimensional paper-based device and flowing in a three-dimensional counterpart were thoroughly investigated, demonstrating that the paper devices from this fabrication process are potentially applicable as analytical devices for biomolecule detection. Fast, easy, and inexpensive parafilm hot press fabrication presents an opportunity for researchers to develop paper-based analytical devices in resource-limited environments. View Full-Text
Keywords: paperfluidics; parafilm; paper-based analytical devices paperfluidics; parafilm; paper-based analytical devices
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MDPI and ACS Style

Kasetsirikul, S.; Clack, K.; Shiddiky, M.J.A.; Nguyen, N.-T. Rapid, Simple and Inexpensive Fabrication of Paper-Based Analytical Devices by Parafilm® Hot Pressing. Micromachines 2022, 13, 48. https://doi.org/10.3390/mi13010048

AMA Style

Kasetsirikul S, Clack K, Shiddiky MJA, Nguyen N-T. Rapid, Simple and Inexpensive Fabrication of Paper-Based Analytical Devices by Parafilm® Hot Pressing. Micromachines. 2022; 13(1):48. https://doi.org/10.3390/mi13010048

Chicago/Turabian Style

Kasetsirikul, Surasak, Kimberley Clack, Muhammad J.A. Shiddiky, and Nam-Trung Nguyen. 2022. "Rapid, Simple and Inexpensive Fabrication of Paper-Based Analytical Devices by Parafilm® Hot Pressing" Micromachines 13, no. 1: 48. https://doi.org/10.3390/mi13010048

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