Next Article in Journal
Realization of Efficient Phosphorescent Organic Light-Emitting Devices Using Exciplex-Type Co-Host
Next Article in Special Issue
Novel Quick Cell Patterning Using Light-Responsive Gas-Generating Polymer and Fluorescence Microscope
Previous Article in Journal
Rapid, Simple and Inexpensive Fabrication of Paper-Based Analytical Devices by Parafilm® Hot Pressing
Previous Article in Special Issue
Fabricating Silicon Resonators for Analysing Biological Samples
Article

Rapid Prototyping of Organ-on-a-Chip Devices Using Maskless Photolithography

1
Department of Anatomy and Embryology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands
2
Department of Human Genetics, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands
3
Department of Neurology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands
4
Department of Microelectronics, Delft University of Technology, 2628 CD Delft, The Netherlands
*
Author to whom correspondence should be addressed.
Academic Editor: Kunal Mitra
Micromachines 2022, 13(1), 49; https://doi.org/10.3390/mi13010049
Received: 10 December 2021 / Revised: 22 December 2021 / Accepted: 27 December 2021 / Published: 29 December 2021
(This article belongs to the Special Issue Micro/Nano Fabrication for Life Sciences)
Organ-on-a-chip (OoC) and microfluidic devices are conventionally produced using microfabrication procedures that require cleanrooms, silicon wafers, and photomasks. The prototyping stage often requires multiple iterations of design steps. A simplified prototyping process could therefore offer major advantages. Here, we describe a rapid and cleanroom-free microfabrication method using maskless photolithography. The approach utilizes a commercial digital micromirror device (DMD)-based setup using 375 nm UV light for backside exposure of an epoxy-based negative photoresist (SU-8) on glass coverslips. We show that microstructures of various geometries and dimensions, microgrooves, and microchannels of different heights can be fabricated. New SU-8 molds and soft lithography-based polydimethylsiloxane (PDMS) chips can thus be produced within hours. We further show that backside UV exposure and grayscale photolithography allow structures of different heights or structures with height gradients to be developed using a single-step fabrication process. Using this approach: (1) digital photomasks can be designed, projected, and quickly adjusted if needed; and (2) SU-8 molds can be fabricated without cleanroom availability, which in turn (3) reduces microfabrication time and costs and (4) expedites prototyping of new OoC devices. View Full-Text
Keywords: SU-8; photoresist; polydimethylsiloxane (PDMS); maskless photolithography; grayscale photolithography; backside exposure; low-cost microfabrication; digital micromirror device (DMD); PRIMO; organ-on-a-chip (OoC) SU-8; photoresist; polydimethylsiloxane (PDMS); maskless photolithography; grayscale photolithography; backside exposure; low-cost microfabrication; digital micromirror device (DMD); PRIMO; organ-on-a-chip (OoC)
Show Figures

Figure 1

MDPI and ACS Style

Kasi, D.G.; de Graaf, M.N.S.; Motreuil-Ragot, P.A.; Frimat, J.-P.M.S.; Ferrari, M.D.; Sarro, P.M.; Mastrangeli, M.; van den Maagdenberg, A.M.J.M.; Mummery, C.L.; Orlova, V.V. Rapid Prototyping of Organ-on-a-Chip Devices Using Maskless Photolithography. Micromachines 2022, 13, 49. https://doi.org/10.3390/mi13010049

AMA Style

Kasi DG, de Graaf MNS, Motreuil-Ragot PA, Frimat J-PMS, Ferrari MD, Sarro PM, Mastrangeli M, van den Maagdenberg AMJM, Mummery CL, Orlova VV. Rapid Prototyping of Organ-on-a-Chip Devices Using Maskless Photolithography. Micromachines. 2022; 13(1):49. https://doi.org/10.3390/mi13010049

Chicago/Turabian Style

Kasi, Dhanesh G., Mees N.S. de Graaf, Paul A. Motreuil-Ragot, Jean-Phillipe M.S. Frimat, Michel D. Ferrari, Pasqualina M. Sarro, Massimo Mastrangeli, Arn M.J.M. van den Maagdenberg, Christine L. Mummery, and Valeria V. Orlova. 2022. "Rapid Prototyping of Organ-on-a-Chip Devices Using Maskless Photolithography" Micromachines 13, no. 1: 49. https://doi.org/10.3390/mi13010049

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop