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Open AccessArticle

Multi-Color Enhanced Fluorescence Imaging of a Breast Cancer Cell with A Hole-Arrayed Plasmonic Chip

1
School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyōgo 669-1337, Japan
2
Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-cho, Takamatsu, Kagawa 761-0395, Japan
*
Author to whom correspondence should be addressed.
Micromachines 2020, 11(6), 604; https://doi.org/10.3390/mi11060604
Received: 30 April 2020 / Revised: 11 June 2020 / Accepted: 20 June 2020 / Published: 22 June 2020
(This article belongs to the Special Issue Micro and Nano Devices for Cell Analysis)
Breast cancer cells of MDA-MB-231 express various types of membrane proteins in the cell membrane. In this study, two types of membrane proteins in MDA-MB-231 cells were observed using a plasmonic chip with an epifluorescence microscope. The targeted membrane proteins were epithelial cell adhesion molecules (EpCAMs) and epidermal growth factor receptor (EGFR), and Alexa®488-EGFR antibody and allophycocyanin (APC)-labeled EpCAM antibody were applied to the fluorescent detection. The plasmonic chip used in this study is composed of a two-dimensional hole-array structure, which is expected to enhance the fluorescence at different resonance wavelengths due to two kinds of grating pitches in a square side and a diagonal direction. As a result of multi-color imaging, the enhancement factor of Alexa®488-EGFR and APC-EpCAM was 13 ± 2 and 12 ± 2 times greater on the plasmonic chip, respectively. The excited wavelength or emission wavelength of each fluorescent agent is due to consistency with plasmon resonance wavelength in the hole-arrayed chip. The multi-color fluorescence images of breast cancer cells were improved by the hole-arrayed plasmonic chip. View Full-Text
Keywords: multi-color imaging; breast cancer cells; surface plasmon; fluorescence microscopy; hole-array multi-color imaging; breast cancer cells; surface plasmon; fluorescence microscopy; hole-array
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MDPI and ACS Style

Yoshida, M.; Chida, H.; Kimura, F.; Yamamura, S.; Tawa, K. Multi-Color Enhanced Fluorescence Imaging of a Breast Cancer Cell with A Hole-Arrayed Plasmonic Chip. Micromachines 2020, 11, 604.

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