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Micromachines
Volume 10
Issue 8
10.3390/mi10080537
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Article

A Lab-on-a-Chip Device Integrated DNA Extraction and Solid Phase PCR Array for the Genotyping of High-Risk HPV in Clinical Samples

by
Cancan Zhu
1,2,
Anzhong Hu
1,
Junsheng Cui
1,
Ke Yang
1,
Xinchao Zhu
1,2,
Yong Liu
1,
Guoqing Deng
1 and
Ling Zhu
1,*
1
Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, 2221 Changjiang Road, Hefei 230000, China
2
Science Island Branch, University of Science and Technology of China, No. 96, JinZhai Road Baohe District, Hefei 230000, China
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(8), 537; https://doi.org/10.3390/mi10080537
Submission received: 17 July 2019 / Revised: 9 August 2019 / Accepted: 13 August 2019 / Published: 15 August 2019
(This article belongs to the Special Issue Biosensors for Pathogen Detection)
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Abstract

Point-of-care (POC) molecular diagnostics play a crucial role in the prevention and treatment of infectious diseases. It is necessary to develop portable, easy-to-use, inexpensive and rapid molecular diagnostic tools. In this study, we proposed a lab-on-a-chip device that integrated DNA extraction, solid-phase PCR and genotyping detection. The ingenious design of the pneumatic microvalves enabled the fluid mixing and reagent storage to be organically combined, significantly reducing the size of the chip. The solid oligonucleotide array incorporated into the chip allowed the spatial separation of the primers and minimized undesirable interactions in multiplex amplification. As a proof-of-concept for POC molecular diagnostics on the device, five genotypes of high-risk human papillomavirus (HPV) (HPV16/HPV18/HPV31/HPV33/HPV58) were examined. Positive quality control samples and HPV patient cervical swab specimens were analyzed on the integrated microdevice. The platform was capable of detection approximately 50 copies of HPV virus per reaction during a single step, including DNA extraction, solid-phase PCR and genotype detection, in 1 h from samples being added to the chip. This simple and inexpensive microdevice provided great utility for the screening and monitoring of HPV genotypes. The sample-to-result platform will pave the way for wider application of POC molecular testing in the fields of clinical diagnostics, food safety, and environmental monitoring.
Keywords: Lab-on-a-chip device; POC; high-risk HPV genotypes; molecular diagnosis Lab-on-a-chip device; POC; high-risk HPV genotypes; molecular diagnosis

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MDPI and ACS Style

Zhu, C.; Hu, A.; Cui, J.; Yang, K.; Zhu, X.; Liu, Y.; Deng, G.; Zhu, L. A Lab-on-a-Chip Device Integrated DNA Extraction and Solid Phase PCR Array for the Genotyping of High-Risk HPV in Clinical Samples. Micromachines 2019, 10, 537. https://doi.org/10.3390/mi10080537

AMA Style

Zhu C, Hu A, Cui J, Yang K, Zhu X, Liu Y, Deng G, Zhu L. A Lab-on-a-Chip Device Integrated DNA Extraction and Solid Phase PCR Array for the Genotyping of High-Risk HPV in Clinical Samples. Micromachines. 2019; 10(8):537. https://doi.org/10.3390/mi10080537

Chicago/Turabian Style

Zhu, Cancan, Anzhong Hu, Junsheng Cui, Ke Yang, Xinchao Zhu, Yong Liu, Guoqing Deng, and Ling Zhu. 2019. "A Lab-on-a-Chip Device Integrated DNA Extraction and Solid Phase PCR Array for the Genotyping of High-Risk HPV in Clinical Samples" Micromachines 10, no. 8: 537. https://doi.org/10.3390/mi10080537

APA Style

Zhu, C., Hu, A., Cui, J., Yang, K., Zhu, X., Liu, Y., Deng, G., & Zhu, L. (2019). A Lab-on-a-Chip Device Integrated DNA Extraction and Solid Phase PCR Array for the Genotyping of High-Risk HPV in Clinical Samples. Micromachines, 10(8), 537. https://doi.org/10.3390/mi10080537

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