Next Article in Journal
Study Effects of Drug Treatment and Physiological Physical Stimulation on Surfactant Protein Expression of Lung Epithelial Cells Using a Biomimetic Microfluidic Cell Culture Device
Next Article in Special Issue
Direct Writing of Copper Micropatterns Using Near-Infrared Femtosecond Laser-Pulse-Induced Reduction of Glyoxylic Acid Copper Complex
Previous Article in Journal
Chaotic Micromixer Based on 3D Horseshoe Transformation
Previous Article in Special Issue
Resistance Change Mechanism of Electronic Component Mounting through Contact Pressure Using Elastic Adhesive
Open AccessArticle

Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers

1
Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan
2
Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan
*
Author to whom correspondence should be addressed.
These authors have equally contributed to this work.
Micromachines 2019, 10(6), 399; https://doi.org/10.3390/mi10060399
Received: 17 May 2019 / Revised: 9 June 2019 / Accepted: 13 June 2019 / Published: 15 June 2019
Engineering of the skeletal muscles has attracted attention for the restoration of damaged muscles from myopathy, injury, and extraction of malignant tumors. Reconstructing a three-dimensional muscle using living cells could be a promising approach. However, the regenerated tissue exhibits a weak construction force due to the insufficient tissue maturation. The purpose of this study is to establish the reconstruction system for the skeletal muscle. We used a cell-laden core-shell hydrogel microfiber as a three-dimensional culture to control the cellular orientation. Moreover, to mature the muscle tissue in the microfiber, we also developed a custom-made culture device for imposing cyclic stretch stimulation using a motorized stage and the fiber-grab system. As a result, the directions of the myotubes were oriented and the mature myotubes could be formed by cyclic stretch stimulation. View Full-Text
Keywords: myoblast; skeletal muscle; core-shell hydrogel fiber; cyclic stretch; engineered muscle myoblast; skeletal muscle; core-shell hydrogel fiber; cyclic stretch; engineered muscle
Show Figures

Figure 1

MDPI and ACS Style

Bansai, S.; Morikura, T.; Onoe, H.; Miyata, S. Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers. Micromachines 2019, 10, 399.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop