Temporal Observation of Adipocyte Microfiber Using Anchoring Device

Yokomizo, Akiyo; Morimoto, Yuya; Nishimura, Keigo; Takeuchi, Shoji

doi:10.3390/mi10060358

Open AccessFeature PaperArticle

Temporal Observation of Adipocyte Microfiber Using Anchoring Device

by

Akiyo Yokomizo

¹,

Yuya Morimoto

^1,2

,

Keigo Nishimura

^1,3

and

Shoji Takeuchi

^1,2,3,4,*

¹

Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan

²

Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

³

Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

⁴

International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo Institutes for Advanced Study (UTIAS), The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

^*

Author to whom correspondence should be addressed.

Micromachines 2019, 10(6), 358; https://doi.org/10.3390/mi10060358

Submission received: 11 May 2019 / Revised: 27 May 2019 / Accepted: 28 May 2019 / Published: 29 May 2019

(This article belongs to the Special Issue Selected Papers from the 9th Symposium on Micro-Nano Science and Technology on Micromachines)

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Abstract

In this paper, we propose an anchoring device with pillars to immobilize an adipocyte microfiber that has a fiber-shaped adipocyte tissue covered by an alginate gel shell. Because the device enabled the immobilization of the microfiber in a culture dish even after its transportation and the exchange of the culture medium, we can easily track the specific positions of the microfiber for a long period. Owing to the characteristics of the anchoring device, we successfully performed temporal observations of the microfiber on the device for a month to investigate the function and morphology of three-dimensional cultured adipocytes. Furthermore, to demonstrate the applicability of the anchoring device to drug testing, we evaluated the lipolysis of the microfiber’s adipocytes by applying reagents with an anti-obesity effect. Therefore, we believe that the anchoring device with the microfiber will be a useful tool for temporal biochemical analyses.

Keywords: microfluidics; biofabrication; adipose tissue; lipolysis

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MDPI and ACS Style

Yokomizo, A.; Morimoto, Y.; Nishimura, K.; Takeuchi, S. Temporal Observation of Adipocyte Microfiber Using Anchoring Device. Micromachines 2019, 10, 358. https://doi.org/10.3390/mi10060358

AMA Style

Yokomizo A, Morimoto Y, Nishimura K, Takeuchi S. Temporal Observation of Adipocyte Microfiber Using Anchoring Device. Micromachines. 2019; 10(6):358. https://doi.org/10.3390/mi10060358

Chicago/Turabian Style

Yokomizo, Akiyo, Yuya Morimoto, Keigo Nishimura, and Shoji Takeuchi. 2019. "Temporal Observation of Adipocyte Microfiber Using Anchoring Device" Micromachines 10, no. 6: 358. https://doi.org/10.3390/mi10060358

APA Style

Yokomizo, A., Morimoto, Y., Nishimura, K., & Takeuchi, S. (2019). Temporal Observation of Adipocyte Microfiber Using Anchoring Device. Micromachines, 10(6), 358. https://doi.org/10.3390/mi10060358

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Temporal Observation of Adipocyte Microfiber Using Anchoring Device

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