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Open AccessArticle

Determination of the Membrane Transport Properties of Jurkat Cells with a Microfluidic Device

1
Department of Fluid Control and Automation, Harbin Institute of Technology, Harbin, Heilongjiang, 150001, China
2
Mechanical Engineering, University of Washington, Seattle WA 98195, USA
3
School of Mechanical and Materials Engineering, Washington State University, Everett, WA 98201, USA
*
Authors to whom correspondence should be addressed.
Micromachines 2019, 10(12), 832; https://doi.org/10.3390/mi10120832
Received: 25 October 2019 / Revised: 22 November 2019 / Accepted: 26 November 2019 / Published: 29 November 2019
(This article belongs to the Special Issue Microfluidics for Cells and Other Organisms, Volume II)
The Jurkat cell is an immortalized line of human acute lymphocyte leukemia cells that is widely used in the study of adoptive cell therapy, a novel treatment of several advanced forms of cancer. The ability to transport water and solutes across the cell membrane under different temperatures is an important factor for deciding the specific protocol for cryopreservation of the Jurkat cell. In this study we propose a comprehensive process for determination of membrane transport properties of Jurkat cell. using a novel microfluidic controlled single cell-trapping system. The osmotic behavior of an individual Jurkat cell to water and dimethyl sulfoxide (DMSO), a commonly used cryoprotective agent (CPA), under constant temperature, was recorded under a microscope utilizing the modified microfluidic system. The images of the Jurkat cell under osmotic change were processed to obtain a relationship between cell volume change and time. The experimental results were fitted using a two-parameter transport numeric model to calculate the Jurkat cell membrane permeability to water and DMSO at room temperature (22 °C). This model and the calculated parameters can help scientists optimize the cryopreservation protocol for any cell type with optimal cryoprotective agents and cooling rate for future experiments.
Keywords: microfluidics; Jurkat cell; cell membrane permeability; osmotic behavior; two-parameter transport formalism microfluidics; Jurkat cell; cell membrane permeability; osmotic behavior; two-parameter transport formalism
MDPI and ACS Style

Yang, T.; Peng, J.; Shu, Z.; Sekar, P.K.; Li, S.; Gao, D. Determination of the Membrane Transport Properties of Jurkat Cells with a Microfluidic Device. Micromachines 2019, 10, 832.

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