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Whole Blood Based Multiparameter Assessment of Thrombus Formation in Standard Microfluidic Devices to Proxy In Vivo Haemostasis and Thrombosis

Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, 6229 ER Maastricht, The Netherlands
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Micromachines 2019, 10(11), 787; https://doi.org/10.3390/mi10110787
Received: 14 October 2019 / Revised: 13 November 2019 / Accepted: 14 November 2019 / Published: 16 November 2019
(This article belongs to the Special Issue Blood-on-a-Chip)
Microfluidic assays are versatile tests which, using only small amounts of blood, enable high throughput analyses of platelet function in several minutes. In combination with fluorescence microscopy, these flow tests allow real-time visualisation of platelet activation with the possibility of examining combinatorial effects of wall shear rate, coagulation and modulation by endothelial cells. In particular, the ability to use blood and blood cells from healthy subjects or patients makes this technology promising, both for research and (pre)clinical diagnostic purposes. In the present review, we describe how microfluidic devices are used to assess the roles of platelets in thrombosis and haemostasis. We place emphasis on technical aspects and on experimental designs that make the concept of “blood-vessel-component-on-a-chip” an attractive, rapidly developing technology for the study of the complex biological processes of blood coagulability in the presence of flow. View Full-Text
Keywords: collagen; platelet; thrombus collagen; platelet; thrombus
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Provenzale, I.; Brouns, S.L.N.; van der Meijden, P.E.J.; Swieringa, F.; Heemskerk, J.W.M. Whole Blood Based Multiparameter Assessment of Thrombus Formation in Standard Microfluidic Devices to Proxy In Vivo Haemostasis and Thrombosis. Micromachines 2019, 10, 787.

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