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Open AccessFeature PaperCommunication

Automated Analysis of Platelet Aggregation on Cultured Endothelium in a Microfluidic Chip Perfused with Human Whole Blood

1
BIOS Lab-on-a-Chip Group, University of Twente, 7522 NH Enschede, The Netherlands
2
Applied Stem Cell Technologies Group, University of Twente, 7522 NB Enschede, The Netherlands
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Authors to whom correspondence should be addressed.
Micromachines 2019, 10(11), 781; https://doi.org/10.3390/mi10110781
Received: 11 October 2019 / Revised: 4 November 2019 / Accepted: 13 November 2019 / Published: 14 November 2019
(This article belongs to the Special Issue Blood-on-a-Chip)
Organ-on-a-chip models with incorporated vasculature are becoming more popular to study platelet biology. A large variety of image analysis techniques are currently used to determine platelet coverage, ranging from manually setting thresholds to scoring platelet aggregates. In this communication, an automated methodology is introduced, which corrects misalignment of a microfluidic channel, automatically defines regions of interest and utilizes a triangle threshold to determine platelet coverages and platelet aggregate size distributions. A comparison between the automated methodology and manual identification of platelet aggregates shows a high accuracy of the triangle methodology. Furthermore, the image analysis methodology can determine platelet coverages and platelet size distributions in microfluidic channels lined with either untreated or activated endothelium used for whole blood perfusion, proving the robustness of the method. View Full-Text
Keywords: triangle threshold; platelet aggregate; platelet coverage; platelet size distribution; automatic threshold; image analysis; thrombosis; thrombosis-on-a-chip; microfluidics; organ-on-a-chip triangle threshold; platelet aggregate; platelet coverage; platelet size distribution; automatic threshold; image analysis; thrombosis; thrombosis-on-a-chip; microfluidics; organ-on-a-chip
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Albers, H.J.; Passier, R.; van den Berg, A.; van der Meer, A.D. Automated Analysis of Platelet Aggregation on Cultured Endothelium in a Microfluidic Chip Perfused with Human Whole Blood. Micromachines 2019, 10, 781.

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