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Integration of Hierarchical Micro-/Nanostructures in a Microfluidic Chip for Efficient and Selective Isolation of Rare Tumor Cells

1
Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, PA 18015, USA
2
Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA
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Department of Materials Science and Engineering, Lehigh University, Bethlehem, PA 18015, USA
4
School of Engineering, Dali University, Dali 671000, Yunnan, China
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Department of Bioengineering, Lehigh University, Bethlehem, PA 18015, USA
*
Authors to whom correspondence should be addressed.
Present address: Energy Efficiency and Materials Research Division, Korea Institute of Energy Research, Daejeon 34129, Korea.
Micromachines 2019, 10(10), 698; https://doi.org/10.3390/mi10100698
Received: 17 September 2019 / Revised: 9 October 2019 / Accepted: 11 October 2019 / Published: 14 October 2019
(This article belongs to the Special Issue Microfluidics-based Liquid Biopsies)
Circulating tumor cells (CTCs) are important clinical markers for both cancer early diagnosis and prognosis. Various techniques have been developed in the past decade to isolate and quantify these cells from the blood while microfluidic technology attracts significant attention due to better controlled microenvironment. When combined with advanced nanotechnologies, CTC isolation performance in microfluidic devices can be further improved. In this article, by extending the wavy-herringbone concept developed earlier in our team, we prepared a hierarchical microfluidic chip by introducing a uniform coating of nanoparticles with anti-epithelial cell adhesion molecule (EpCAM) on wavy microgrooves. This hierarchical structured platform not only maintains the capture purity of the wavy-herringbone structure but improves the capture efficiency thanks to the larger surface area to volume ratio brought by nanoparticles. Our results demonstrated a capture efficiency of almost 100% at a low shear rate of 60/s. Even at a higher shear rate of 400/s, the hierarchical micro/nanostructures demonstrated an enhancement of up to ~3-fold for capture efficiency (i.e., 70%) and ~1.5-fold for capture purity (i.e., 68%), compared to wavy-herringbone structures without nanoparticle coating. With these promising results, this hierarchical structured platform represents a technological advancement for CTC isolation and cancer care. View Full-Text
Keywords: microfluidics; nanoparticles; circulating tumor cell (CTC) isolation microfluidics; nanoparticles; circulating tumor cell (CTC) isolation
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Wang, S.; Cho, Y.; Cheng, X.; Yang, S.; Liu, Y.; Liu, Y. Integration of Hierarchical Micro-/Nanostructures in a Microfluidic Chip for Efficient and Selective Isolation of Rare Tumor Cells. Micromachines 2019, 10, 698.

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