Next Article in Journal
A Microfluidic Deformability Assessment of Pathological Red Blood Cells Flowing in a Hyperbolic Converging Microchannel
Previous Article in Journal
Improved Memory Properties of Graphene Oxide-Based Organic Memory Transistors
Previous Article in Special Issue
Manipulation of Magnetic Beads with Thin Film Microelectromagnet Traps
Open AccessArticle

Continuous-Flow Separation and Efficient Concentration of Foodborne Bacteria from Large Volume Using Nickel Nanowire Bridge in Microfluidic Chip

1
Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China
2
Key Laboratory on Modern Precision Agriculture System Integration Research, Ministry of Education, China Agricultural University, Beijing 100083, China
3
College of Food Science and Technology, Hainan University, Haikou 570228, China
4
Key Laboratory of Biorheological Science and Technology of the Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China
5
Department of Physics, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
*
Author to whom correspondence should be addressed.
This author contributes equally to the first author.
Micromachines 2019, 10(10), 644; https://doi.org/10.3390/mi10100644
Received: 23 August 2019 / Revised: 11 September 2019 / Accepted: 23 September 2019 / Published: 25 September 2019
(This article belongs to the Special Issue Magnetic Biosensors)
Separation and concentration of target bacteria has become essential to sensitive and accurate detection of foodborne bacteria to ensure food safety. In this study, we developed a bacterial separation system for continuous-flow separation and efficient concentration of foodborne bacteria from large volume using a nickel nanowire (NiNW) bridge in the microfluidic chip. The synthesized NiNWs were first modified with the antibodies against the target bacteria and injected into the microfluidic channel to form the NiNW bridge in the presence of the external arc magnetic field. Then, the large volume of bacterial sample was continuous-flow injected to the channel, resulting in specific capture of the target bacteria by the antibodies on the NiNW bridge to form the NiNW–bacteria complexes. Finally, these complexes were flushed out of the channel and concentrated in a lower volume of buffer solution, after the magnetic field was removed. This bacterial separation system was able to separate up to 74% of target bacteria from 10 mL of bacterial sample at low concentrations of ≤102 CFU/mL in 3 h, and has the potential to separate other pathogenic bacteria from large volumes of food samples by changing the antibodies. View Full-Text
Keywords: immunomagnetic separation; nickel nanowires bridge; large-volume sample; microfluidic chip; foodborne bacteria immunomagnetic separation; nickel nanowires bridge; large-volume sample; microfluidic chip; foodborne bacteria
Show Figures

Figure 1

MDPI and ACS Style

Huo, X.; Chen, Q.; Wang, L.; Cai, G.; Qi, W.; Xia, Z.; Wen, W.; Lin, J. Continuous-Flow Separation and Efficient Concentration of Foodborne Bacteria from Large Volume Using Nickel Nanowire Bridge in Microfluidic Chip. Micromachines 2019, 10, 644.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop