Continuous-Flow Separation and Efficient Concentration of Foodborne Bacteria from Large Volume Using Nickel Nanowire Bridge in Microfluidic Chip
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Fabrication of the Microfluidic Chip
2.3. Synthesis of the Nickel Nanowires
2.4. Functionalization of the Nickel Nanowires
2.5. Culture and Enumeration of the Target Bacteria
2.6. Separation and Concentration of the Target Bacteria
3. Results
3.1. Simulation of the Magnetic Field
3.2. Characterization of the Nickel Nanowires
3.3. Comparison of the NiNWs and MNPs for Conventional Magnetic Separation of Bacteria
3.4. Separation of the Pure Bacteria from Large Volume in Microfluidic Chip
3.5. Separation of the Target Bacteria in the Spiked Chicken Samples
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Huo, X.; Chen, Q.; Wang, L.; Cai, G.; Qi, W.; Xia, Z.; Wen, W.; Lin, J. Continuous-Flow Separation and Efficient Concentration of Foodborne Bacteria from Large Volume Using Nickel Nanowire Bridge in Microfluidic Chip. Micromachines 2019, 10, 644. https://doi.org/10.3390/mi10100644
Huo X, Chen Q, Wang L, Cai G, Qi W, Xia Z, Wen W, Lin J. Continuous-Flow Separation and Efficient Concentration of Foodborne Bacteria from Large Volume Using Nickel Nanowire Bridge in Microfluidic Chip. Micromachines. 2019; 10(10):644. https://doi.org/10.3390/mi10100644
Chicago/Turabian StyleHuo, Xiaoting, Qi Chen, Lei Wang, Gaozhe Cai, Wuzhen Qi, Zengzilu Xia, Weijia Wen, and Jianhan Lin. 2019. "Continuous-Flow Separation and Efficient Concentration of Foodborne Bacteria from Large Volume Using Nickel Nanowire Bridge in Microfluidic Chip" Micromachines 10, no. 10: 644. https://doi.org/10.3390/mi10100644