Next Article in Journal
Current Review of Mycotoxin Biodegradation and Bioadsorption: Microorganisms, Mechanisms, and Main Important Applications
Next Article in Special Issue
Risk Assessment of Combined Exposure to Multiple Chemicals at the European Food Safety Authority: Principles, Guidance Documents, Applications and Future Challenges
Previous Article in Journal
Analytical Validation of a Direct Competitive ELISA for Multiple Mycotoxin Detection in Human Serum
Previous Article in Special Issue
Multi-Mycotoxin Long-Term Monitoring Survey on North-Italian Maize over an 11-Year Period (2011–2021): The Co-Occurrence of Regulated, Masked and Emerging Mycotoxins and Fungal Metabolites
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Industrial-Scale Cleaning Solutions for the Reduction of Fusarium Toxins in Maize

by
Michelangelo Pascale
1,*,
Antonio F. Logrieco
2,
Vincenzo Lippolis
2,
Annalisa De Girolamo
2,
Salvatore Cervellieri
2,
Veronica M. T. Lattanzio
2,
Biancamaria Ciasca
2,
Anna Vega
3,
Mareike Reichel
4,
Matthias Graeber
3 and
Katarina Slettengren
3,*
1
Institute of Food Sciences (ISA), National Research Council of Italy (CNR), 83100 Avellino, Italy
2
Institute of Sciences of Food Production (ISPA), National Research Council of Italy (CNR), 70126 Bari, Italy
3
Bühler AG, 9240 Uzwil, Switzerland
4
Eurofins WEJ Contaminants, 21079 Hamburg, Germany
*
Authors to whom correspondence should be addressed.
Toxins 2022, 14(11), 728; https://doi.org/10.3390/toxins14110728
Submission received: 23 September 2022 / Revised: 15 October 2022 / Accepted: 21 October 2022 / Published: 25 October 2022

Abstract

:
Grain cleaning is the most effective non-destructive post-harvest mitigation strategy to reduce high levels of mycotoxins on account of the removal of mold-infected grains and grain fractions with high mycotoxin content. In this study, the reduction in the concentration of some co-occurring Fusarium toxins in maize, namely deoxynivalenol (DON), zearalenone (ZEA) and fumonisins B1 and B2 (FBs), was evaluated at an industrial-scale level by mechanical removal (sieving and density separation) of dust, coarse, small, broken, shriveled and low-density kernels and/or optical sorting of defected kernels. Samples were dynamically collected according to the Commission Regulation No. 401/2006 along the entire process line. Mycotoxin analyses of water–slurry aggregate samples were performed by validated LC methods. Depending on the contamination levels in raw incoming maize, the overall reduction rates ranged from 36 to 67% for DON, from 67 to 87% for ZEA and from 27 to 67% for FBs. High levels of DON, ZEA and FBs were found in all rejected fractions with values, respectively, up to 3030%, 1510% and 2680%, compared to their content in uncleaned maize. Results showed that grain cleaning equipment based on mechanical and or optical sorting technologies can provide a significant reduction in Fusarium toxin contamination in maize.
Key Contribution: The paper reports industrial-scale cleaning solutions for the reduction in Fusarium toxins contamination in maize.

Graphical Abstract

1. Introduction

Pathogenic fungi of the genus Fusarium are widespread in cereal-growing areas worldwide causing severe crop yield losses with consequent economic losses. In addition, several Fusarium species colonizing cereals, under favorable environmental conditions, can produce and accumulate mycotoxins in grains, of which some are of notable concern for human and animal health [1,2,3,4].
Deoxynivalenol (DON), zearalenone (ZEA) and fumonisins B1 and B2 (FBs) are well-known Fusarium toxins associated to cereals, including maize. Their toxic effects on humans and animals have been studied for many years and several scientific opinions and evaluations on risks related to exposure estimates have been carried out by several authorities [5,6,7,8,9,10]. The International Agency for Research on Cancer (IARC) has classified fumonisin B1 (FB1) as possibly carcinogenic to humans (Group 2B), whilst DON and ZEA are not classifiable as to their carcinogenicity to humans (Group 3) [11].
In order to protect human and animal health, maximum limits for Fusarium toxins (mainly DON, ZEA and FBs) in cereals and cereal-based products have been established/recommended in several countries worldwide, including the European Union [12,13,14,15].
Current recommended practices for the prevention and reduction in Fusarium toxin contamination in cereals are based on Good Agricultural Practices (GAP) and Good Manufacturing Practices (GMP) [16,17,18]. However, in some agricultural seasons the levels of Fusarium toxins in harvested crops could be higher than the maximum permitted levels due to climatic conditions favorable to the growth of toxigenic fungi, with consequent mycotoxin accumulation in grains. The contaminated batches, depending on the levels of contamination, should be destroyed or used as animal feed or as biomass for biofuel production with consequent economic losses for the farmers. To avoid this, several post-harvest decontamination strategies using physical, chemical or biological approaches have been investigated with the aim of reducing mycotoxin contamination in grains [19,20,21,22,23,24,25,26,27]. In particular, physical methods (i.e., sieving, aspiration, gravity separation, manual or optical sorting) removing visibly moldy, low-density, infected, colored/discolored, broken and/or damaged kernels, as well as fine materials and dust, have shown to be effective in reducing mycotoxins in cereals and other commodities [28,29,30,31,32,33,34,35].
Several studies on the fate of Fusarium mycotoxins during the processing of wheat have been carried out at laboratory or pilot level showing that cleaning and sorting steps are effective solution in removing toxins contaminated fractions. However, the effects of mycotoxins reduction significantly varied depending on the level of contamination and the amount of rejected fractions during the processing [36,37,38,39]. A recent study has showed that the removal of small kernels by a laboratory sieve also reduces the content of Fusarium mycotoxins in oats thus improving the grain quality [40].
Regarding maize, a comprehensive review on the fate of mycotoxins, including the Fusarium toxins DON, ZEA and FBs, during the primary food processing of maize has been recently published by Schaarschmidt and Fauhl-Hassek [41]. Changes in Fusarium toxins DON, ZEA and FBs level during cleaning of maize largely varied depending on batches and type of mycotoxins, as well as processing procedures. As an example, a reduction in FBs concentrations between 30–90% in small batches of maize was obtained by manual sorting [42,43,44] whereas cleaning based on sieving reduced FBs concentrations by 25–70% [45,46,47]. Analogously to manual sorting, optical sorting has been demonstrated to efficiently reduce the levels of fumonisins (and aflatoxins) in maize [48,49].
Dry milling has shown to significantly reduce DON and ZEA concentration in grits and flour, as well as other minor Fusarium toxins including 3-acetyl-DON (3-ADON), 15-acetyl-DON (15-ADON), nivalenol (NIV), T-2 and HT-2 toxin and moniliformin (MON) in maize [50,51].
Industrial processing may not always reflect what is observed in laboratory and pilot-scale experiments; however, the few studies carried out at industrial level have shown the efficacy of cleaning and sorting in reducing the level of mycotoxins in cereal grains. In particular, the efficacy of maize cleaning steps on aflatoxin B1 (AFB1) and FB1 contamination levels has been evaluated in an industrial scale process aimed to assess the distribution of these mycotoxins in fractions derived from the dry-milling of two maize lots contaminated at different levels. The cleaning step reduced AFB1 and FB1 levels by 8–57% and 11–34%, respectively [52]. In a similar study aimed to evaluate the distribution of FBs in maize dry-milling products and by-products, grain-cleaning using a dry stoner, an intensive horizontal scourer, a vibrating aspirator and an optical sorter reduced FBs by about 42% [53]. More recently, a continuous cleaning line combining both mechanical and optical sorting technologies at industrial scale level has been shown to be an efficient solution for reducing aflatoxins (AFBs) in maize. Batches of biomass/feed quality maize contaminated by AFBs were converted into feed/food quality maize. Aflatoxin reductions from 65% to 84% with respect to the uncleaned products were observed [54]. Very high levels of AFBs (up to 490 µg/kg) were found in the rejected fractions, showing the effectiveness of removing small and broken kernels, dust/fine particles, defected kernels for reducing mycotoxin contamination in maize. Industrial cleaning processes involving scouring, aspiration, and optical sorting reduced mean MON content in maize by 47%. Similarly, a content mean reduction in 52% was achieved for FBs contamination [51].
At our knowledge, to date, no targeted study on the assessment of the effectiveness of cleaning/sorting technologies in industrial grain processing for the simultaneous reduction in legislated Fusarium toxins (i.e., DON, ZEA and FBs) has been carried out. The aim of this study was to evaluate the efficacy of industrial-scale dry cleaning equipment in reducing DON, ZEA and FBs in naturally contaminated maize. Two studies have been carried out at industrial level in Italy and Spain, respectively, for investigating the effect of cleaning solutions on the reduction in Fusarium mycotoxins, i.e., DON, ZEA and FBs in maize. The first study was carried out to evaluate the performances of a high-capacity optical sorting machine in removing contaminated kernels from naturally highly contaminated maize batches; the second study was aimed to evaluate the performance of a cleaning industrial line combining both mechanical (separator, aspirator, concentrator) and optical sorting in reducing the content of the above-mentioned Fusarium toxins in maize. The mass balance of the three mycotoxins after cleaning was carried out in order to verify the accuracy of the results.

2. Results

2.1. First Study

In the first study the total amount of reject fractions with coloured/discoloured and defective maize kernels of the three batches of maize naturally contaminated with DON, ZEA and FBs accounted to 5% (Table 1).
Mycotoxin levels in the three batches ranged from 3200 to 17,400 µg/kg for DON, from 660 to 4460 µg/g for ZEA and from 2520 to 6540 µg/kg for FBs with batch C being the least contaminated one for all mycotoxins (Table 2). The fraction rejected from the sorter contained higher levels of mycotoxins compared to the unprocessed maize (incoming fraction). In the case of DON and FBs, the increment of mycotoxin levels was quite similar in the three batches, accounting to approximately 800% for DON and 250% for FBs. In the case of ZEA a higher variability was observed within the three batches, and values ranged from 400 to 1400%. Consequently, low levels of mycotoxins were observed in the cleaned maize with a reduction between 44–67% of DON, 67–87% of ZEA and 27–28% of FBs, with respect to their content in incoming maize (Table 2).

2.2. Second Study

In the second study two batches (17 tons each) of maize naturally contaminated with DON, ZEA, FB1 and FB2 were processed by a cleaning industrial line comprising a separator coupled with an aspirator, a concentrator and an optical sorter (mass flow rate: 17 tons/h). Percentages of rejected fractions containing broken/damaged kernels, fine and foreign materials collected from separator, aspirator, concentrator and optical sorter ranged from 0.1 to 3% with separator providing the highest rejected amount (Table 1). The total amount of reject fractions accounted to 6.4% and 4.0% for batches A (maize from France) and B (maize from Spain), respectively. Mycotoxin levels determined in the sampled fractions of the two replicates of the batches A and B are reported in Table 3. Levels in the uncleaned maize were between 220–350 µg/kg for DON, 40–55 µg/kg for ZEA and 1705–1765 µg/kg for FBs. In all cases, these levels were far below the EU maximum levels established for these mycotoxins in uncleaned maize (i.e., 1750 µg/kg for DON, 350 µg/kg for ZEA and 4000 µg/kg for FBs [13]. After the different cleaning steps through TASTM, concentrator MTCBTM and SORTEX® Z+, mycotoxins levels in the cleaned maize were 10 µg/kg for ZEA and between 105–200 µg/kg for DON and 580–1160 µg/kg for FBs, corresponding to a reduction in 75–82%, 36–52% and 34–67%, respectively. The reduction in DON and ZEA between the two batches of maize was similar, while in the case of FBs it was higher in the Spanish batch.
This reduction corresponded to an incremented concentration of mycotoxins in the rejected fractions (fractions 2–6) for all targeted mycotoxins. Specifically, the content (%) of mycotoxins in the fractions from batches A and B of maize was between 162–417% in fractions 2, 550–2683% in fractions 3, 444–2194% in fractions 4, 962–3026% in fractions 5 and between 267–3457% in fractions 6, with respect to the raw material (uncleaned maize) (Figure 1), with a more evident effect in maize from Spain (batch B).

2.3. Mass Balance

A mass balance calculation was applied to quantitatively estimate the distribution of DON, ZEA and FBs among fractions obtained during the industrial-scale maize cleaning as compared to raw material. Mass balance results for study 1 (batches A, B and C) and study 2 (batches A1, A2, B1 and B2) are reported in Table 4. Overall, the mass balance in the study 1 was between 80–108% for the three mycotoxins. Similar results were also obtained in the study 2 for DON (79–105%) and FBs (88–114%), while lower and slightly more variable results were obtained in the case of ZEA (37–62%). These latest results were probably related to the low contamination levels of ZEA in the starting maize for study 2 (40–55 µg/kg) that were close to the limit of quantification of the method (35 µg/kg), with respect to those in study 1 (661–4460 µg/kg). The low levels of ZEA contamination in batches A1, A2, B1 and B2 led to a higher analytical error. By excluding ZEA results in study 2, the overall results indicated a reliable sampling plan, a good accuracy of analytical data and a suitability of the industrial-scale cleaning studies described in the present paper.

3. Discussion

Cleaning of cereals allows the removal of foreign materials and broken, shrivelled, damaged and low-density kernels. The process is commonly used before storage and/or milling. This physical procedure has been shown to be effective in reducing mycotoxin contents by removing highly contaminated material at the early stage of the food and feed chain and to prevent fungal colonization during storage.
Traditional cleaning techniques include the manual sorting-out of small, broken, and low-density grains. Although it can be quite effective in reducing Fusarium toxins, the automated optical sorting represents a more specific strategy for removing Fusarium-infected grains and related toxins, even though they require more cost-intensive equipment [26,36,41]. Results reported in the present paper confirm that optical sorting represents an effective strategy for reducing mycotoxins along the entire chain of industrial maize processing. Furthermore, the integrated process solutions removing mechanically Fusarium-infected maize kernels, as well as maize fractions based on their characteristics (i.e., specific gravity and optical properties), makes this cleaning procedure suitable for managing Fusarium mycotoxin in maize. However, in general, most of the literature data describe the combined effect of sorting, cleaning and milling on the reduction in mycotoxins in wheat and maize. The majority of data are for DON and FBs, and at lesser extent for other Fusarium toxins such as T2/HT2, 3-ADON, 15-ADON and NIV, while limited information is available for ZEA [24,26,36,39,41,55]. In a study undertaken to examine the efficiency of a high-speed optical sorting of wheat kernels, an average reduction in DON contamination levels of 51%, with respect to the concentration in unsorted wheat was observed. Successive cleaning steps were successful at further reducing the concentration of DON [56]. In another work, the removal of Fusarium-contaminated grains from wheat using optical sorters successfully reduced the DON and NIV concentrations in the cleaned wheat by approximately 50% [57]. Another application of optical sorting was reported by Carmack et al. [33] for the selection of breeding lines of wheat with enhanced Fusarium head blight resistance, i.e., with lower levels of DON and Fusarium-damaged kernels. Results obtained herein on the effect of sorting and cleaning on DON removal from maize (36–67%) are in line with the range reported in the literature, while a similar comparison is not reliable for ZEA because of limited data availability on it. A significant reduction in DON levels was also observed after the application of colour sorting to 20 different samples of wheat, with an average level of approximately 12% [34].
In a study evaluating the redistribution of 16 Fusarium toxins, including DON and ZEA, dry milling of two batches of maize at industrial level, a marked increment of mycotoxin levels (396–807% for DON and 1400–1743% for ZEA) was found in screenings (containing small and broken and, therefore, heavily mouldy and toxin contaminated kernels) compared to the mycotoxin content in the starting maize [50].
Concerning FBs, in a study carried out using a dual wavelength high-speed commercial sorter on white maize contaminated at different levels, a reduction ranging from 29 to 96% of FBs was observed by rejecting from 4 to 9% of maize [49]. In another work, Westhuizen et al. [44] described the application of hand-sorting of home-grown maize kernels under laboratory-controlled conditions by reporting a removal of FBs by 71% with an additional 13% after a 10-min ambient temperature water wash. During the dry-milling process of four lots of maize, the cleaning operation, mainly carried out using an optical sorter, reduced the level of FBs in the cleaned maize from 43% to 76% depending on the specific set up of the optical sorter used for each lot of maize which was milled in different growing seasons [51]. A low-cost sorter prototype (the ‘DropSort’ device) separating maize based on kernel bulk density was effective in reducing FBs in maize. In a further study, the DropSorter was combined with size sorting to separate grain samples into large + heavy kernels and small + light kernels and FBs reduction was up to 98% [32].
Data on the effects of industrial-scale optical sorting on simultaneous reduction in Fusarium toxins in cereals are very limited, and only few describe their application to FBs. Vanara et al. [53] reported a 42% reduction in FBs after cleaning of maize during the milling process using a dry stoner, an intensive horizontal scourer, a vibrating aspirator and an optical sorter. Similarly, two different batches of maize obtained after cleaning steps including a separator with aspirator, a dry de-stoner and an intensive scourer coupled with an aspirator, achieved to lower the FB1 level by 11% and 34%, respectively, compared to the uncleaned maize [52]. In a study aimed to evaluate the fate of AFBs and FBs during the processing of maize for the production of makume and owo, maize-based foods common in Benin (West Africa), the sorting and winnowing of different batches of maize reduced FBs by 69% and 44%, respectively [42]. A certain agreement of results for FBs removal after sorting was observed among results reported in the present study (27–67%, Table 3 and Table 4) and the literature cited herein (11–98%) [32,42,44,49,51,52,53]. On the other hand, Generotti et al. [58], reported that the maize cleaning step through a system characterized by a separator with aspirator and sieve, a magnet and an optical sorter did not cause a reduction in FBs content in cleaned maize.
The effect of cleaning in terms of mycotoxin reduction can greatly vary depending on the levels of contamination in the raw material and from the quality of a batch, which can be affected by the cultivar, weather conditions and cultivation practices [26,36,41]. Although in the present study the effect of contamination levels cannot be evaluated on account of the comparable mycotoxins content in the batches used for study 2, the higher percentage of total rejected fractions observed in French maize with respect to Spanish maize (Table 1) can be justified by their different origin having potential differences in terms of weather conditions and cultivation practices, cultivars and harvest techniques. The high variability in mycotoxin distribution among fractions after cleaning resulted from the sampling plan, as well as from the amount of rejected fractions. The effects of Fusarium toxins reduction were more evident in Spanish maize (batch B), with respect to French maize (batch A) (Figure 1). A possible explanation of these results could be related to the different total amount of discarded fractions during the overall cleaning process between the two batches, i.e., 6.4% for French maize and 4% for the Spanish one, also because the mycotoxin levels in the two batches of uncleaned maize materials were quite similar. Moreover, a higher increment of concentration in the fraction 6 rejected from the sorter was observed for DON as compared to that of study 1. Maybe this behaviour was attributable to the lower DON level in the uncleaned maize of study 2 (less than 400 µg/kg) compared to that in the uncleaned maize of study 1 (3200–17,400 µg/kg, depending on the batch). On the other hand, ZEA results in the sorter rejected fraction confirmed those reported for study 1, either in terms of high variability than in terms of increase in concentration (up to approximately 1400%). Similarly, results for FBs increase in the rejected fractions were in the same range as those observed for study 1. However, the rejected fraction from the sorter (fraction 6) showed a lower increase in FBs concentration compared to the other rejected fractions (i.e., from 2 to 5).

4. Conclusions

The present study evaluates the effect of industrial-scale cleaning equipment on the simultaneous reduction in DON, ZEA and FBs in uncleaned maize. Specifically, two studies were carried out; in the first study maize samples were cleaned by an optical sorter which removed foreign bodies and kernels with visual defects; in the second one, maize samples were mechanically cleaned with a separator, an aspirator and a density separation machine and then optically sorted. Starting materials (uncleaned maize), final materials (cleaned maize) and rejected fractions were analysed for mycotoxins content. A reduction in mycotoxins was observed in both studies. In particular, the first study clearly showed the effectiveness of the use of optical sorting at industrial level in simultaneously reducing DON, ZEA and FBs in maize. Similarly, the second study showed that the combination of mechanical cleaning and optical sorting were able to reduce mycotoxins level in maize. In both cases, mycotoxins were accumulated in the rejected fractions. The calculated mass balance confirmed the reliability and accuracy of the tested approaches.
Results reported herein confirm that cleaning procedures based on mechanical and/or optical sorting technologies are effective in the reduction in DON, ZEA and FBs in uncleaned maize. The industrial scale of these experiments makes the obtained results very reliable by indicating that the application of cleaning procedures contributes to fulfil food safety requirements and could be part of a successful strategy for managing Fusarium mycotoxins in maize.

5. Materials and Methods

5.1. Materials and Reagents

Analytical-grade solvents, o-phthaldialdehyde (OPA), 2-mercaptoethanol, sodium tetraborate and phosphate buffered saline (PBS) tablet, were purchased either from Mallinckrodt Baker (Milan, Italy) or Sigma (St. Louis, MO, USA). Ultrapure water was produced by a Millipore Milli-Q system (Millipore, Bedford, MA, USA). Standards of DON, ZEA, FB1 and FB2 were purchased from Sigma. DONTest, ZearalaTest and FumoniTestTM Wide Bore immunoaffinity columns were purchased from Vicam L.P. (A Waters Business, Milford, MA, USA).

5.2. Samples and Cleaning Processes

In the first study three batches of maize naturally contaminated with DON, ZEA, FB1 and FB2 (25 tons each), namely A, B and C, slightly pre-cleaned by mechanical sorting were cleaned by an optical sorter (SORTEX A5 BRBX, Buhler AG, Uzwil, Switzerland). Sortex A5 consists of five modules, each of a width of 300 mm, with the machine capable of processing a total of 10–20 tons per hour. Each module of the sorter has visible and shortwave Infrared (InGaAs) cameras both front and rear. The colour cameras were setup to detect colour and spot defects from the maize. The IR cameras were setup to remove the challenging foreign material not targetable with the visible system. Once an object had been identified as defective the decisions were sent to a bank of valves and a targeted compressed air pulse was used to remove the object from the product stream, thus ensuring maximum food safety of the cleaned product. The study was carried out for research purposes in North Italy in 2015 in a plant able to process 25 tons of raw maize per hour. The scheme of the industrial sorting line is shown in Figure 1.
The second study was carried out in Spain in 2018 in a plant able to process 17 tons/h of raw maize. The scheme of the industrial cleaning line is shown in Figure 2.
In study 2, two batches of maize (17 tons each), namely A (from France) and B (from Spain), naturally contaminated with DON, ZEA, FB1 and FB2 were mechanically cleaned and optically sorted using industrial-scale cleaning equipment. The equipment included a sieving machine (TASTM), consisting of a separator and an aspirator, a density separation machine, consisting of a concentrator (MTCBTM), as well as an optical sorting machine (SORTEX® Z+). The sieving separator removed small/broken/fine materials (fraction 2) and coarse/fine (fraction 3) kernels, the aspirator removed dust and husk particles (fraction 4), while the concentrator classified maize fractions into high-, mixed- and low-density materials and eliminated lighter maize fractions (fraction 5). The sorter was equipped with an enhanced InGaAs camera and climate control and removed kernels with visual signs of contamination (fraction 6). The outlet of the sorter was the cleaned maize, representing the end product (fraction 7).

5.3. Sampling

Samples were taken dynamically according to the Commission Regulation No. 401/2006 along the entire process line, including cleaned and rejected project streams [59].
For study 1 incremental samples ranging from 10 to 100 (100–300 g each) were collected from the sorter at regular intervals according to the sampling protocol. For study 2 incremental samples ranging from 5 to 60 (100–300 g each) were collected for each batch of maize at sampling points 1–7 from opening slits of the plants at regular intervals. Two replicates per batch, for a total of four studies, were carried out, i.e., A1, A2, B1 and B2.
For both studies, the number of incremental samples and the weight of the aggregate samples submitted to analysis are reported in Table 5. Sampling points are indicated in Figure 2 and Figure 3. Samples were maintained at +4 °C until the mycotoxin analysis was performed.

5.4. Mycotoxins Analysis

To minimize subsampling errors aggregate samples of fractions weights higher than 5 kg were slurry-mixed with water in matrix:water ratio 1:1 (w:w) for 10 min using the Silverson EX high share mixer (Silverson Machines Ltd., Waterside, Chesham, UK) as described by Pascale et al. [54]. For aggregate samples of 1–2 kg, an Ultra Turrax IKA T25 (IKA Werke GmbH & Co. KG., Staufen, Germany) was used for preparation of slurries and for recovery experiments. Unprocessed maize samples (study 2) were preliminarily analysed by LC-MS/MS [60] to screen the simultaneous occurrence of mycotoxins. Low levels of AFBs (up to 0.25 µg/kg), ochratoxin A (up to 0.15 µg/kg), T2 toxin (up to 10 µg/kg), HT2 toxin (up to 15 µg/kg) and beauvericin (up to 5 µg/kg) were observed. Then, the analysis of DON, ZEA, FB1 and FB2 in the water–slurry samples was carried out at CNR using validated HPLC methods.

5.4.1. Analysis of DON

Analysis of DON was performed according to [61] for the determination of DON in cereals and cereal products with some modifications. Briefly, aliquots of slurry (50 g) were extracted with 75 mL PBS by blending at high speed for 2 min (Sorvall Omnimixer). The extracts were filtered through Whatman No. 4 filter paper (Whatman, Maidstone, UK) followed by glass microfiber filter Whatman GF/A (Whatman). One ml of filtered extract was cleaned up through DONTest immunoaffinity column (VICAM) at a rate of about 1 drop/second. The column was washed with 2 × 5 mL water at a flow rate of 1–2 drops/s and DON was eluted with 2 × 0.75 mL methanol in a 4-mL vial. The eluted extract was gently dried under a nitrogen stream at about 50 °C and reconstituted with 250 μL of LC mobile phase (water:methanol, 85:15, v/v). An aliquot of 10 µL of reconstituted extract (equivalent to 0.01 g sample matrix) was injected into the chromatographic apparatus by full loop injection. The LC system consisted of the ultra-high performance liquid chromatography instrument (UHPLC) Agilent 1290 Infinity (Agilent Technologies, Santa Clara, CA, USA) equipped with a pump, degasser, column oven, auto sample injector and a PDA detector. The chromatographic separation of DON was obtained using a Zorbax Eclipse XDB-C18 column (150 mm × 2.1 mm, 1.8 µm) and an isocratic mobile phase of water:methanol (85:15, v/v) at a flow rate of 0.4 mL/min. With these conditions, deoxynivalenol eluted within 9 min. Limit of detection (LOD), based on a signal to noise ratio of 3:1, was 10 µg/kg DON. Limit of quantification (LOQ), based on a signal to noise ratio of 10:1, was 35 µg/kg DON.

5.4.2. Analysis of ZEA

Analysis of ZEA was performed according to [62] for the determination of ZEA in barley, maize and wheat flour, polenta, and maize-based baby food with some modifications. Briefly, aliquots of slurry (40 g) were extracted with 180 mL acetonitrile by blending at high speed for 2 min (Sorvall Omnimixer). The extracts were filtered through Whatman No. 4 filter paper (Whatman) and 10 mL of filtered extract was diluted with 90 mL PBS and filtered through Whatman GF/A (Whatman). Then, 20 mL of filtered extract was cleaned up through ZearalaTest immunoaffinity column (VICAM) at a rate of about 1 drop/second. The column was washed with 2 × 5 mL water at a flow rate of 1–2 drops/s and mycotoxins were eluted with 2 × 0.75 mL methanol in a 4-mL vial. After drying under a nitrogen stream at about 50 °C, the extract was reconstituted with 250 μL of LC mobile phase (water:acetonitrile:methanol, 46:46:8, v/v/v). An aliquot of 100 µL of reconstituted extract (equivalent to 0.08 g sample matrix) was injected into the chromatographic apparatus by full loop injection. The LC system consisted of a high-performance liquid chromatography instrument (HPLC) Agilent 1100 Series (Agilent Technologies) equipped with a pump, degasser, column oven, auto sample injector and a fluorescent detector (λex = 274 nm; λem = 440 nm). The chromatographic separation of ZEA was obtained using a Symmetry C18 column (150 mm × 4.6 mm, 5 µm) (Waters) and an isocratic mobile phase (water:acetonitrile:methanol, 46:46:8, v/v/v) at a flow rate of 1.0 mL/min. With these conditions, ZEA eluted within 7 min. The LOD and LOQ values of the method were 10 µg/kg and 35 µg/kg ZEA, respectively.

5.4.3. Analysis of FB1 and FB2

Extraction of FBs from maize was carried out according to [63] with some modifications. Briefly, aliquots of slurry (40 g) were extracted with a mixture (40 mL) of methanol:acetonitrile:water (31.25:31.25:37.50, v/v/v) by shaking for 20 min. After filtration through Whatman No. 4 filter paper (Whatman) the remaining solid material was extracted again with the extraction solvent (40 mL) by shaking for 20 min and the extract was filtered through the same filter paper. The two extracts were combined, and an aliquot of filtrate (10 mL) was diluted with PBS (40 mL) and filtered through Whatman GF/A (Whatman). Then a volume of filtered extract (10 mL) was cleaned up through FumoniTestWB immunoaffinity column (VICAM). After elution, the column was washed with 10 mL PBS and FBs were eluted with 2 × 1 mL methanol followed by 2 × 1 mL water in a 4-mL vial. Then the extract was dried under a nitrogen stream at about 50 °C and reconstituted with 500 μL of water:acetonitrile (70:30, v/v). Sample extracts were derivatised with OPA reagent and analysed by HPLC according to the procedure described by De Girolamo et al. [64]. With these conditions, retention times of FB1 and FB2 were about 17 and 24 min, respectively. The LOD values were 70 µg/kg for FB1 and 40 µg/kg for FB2, while LOQ values were 240 µg/kg for FB1 and 140 µg/kg for FB2.

5.5. Mass Balance

For each mycotoxin and for each study, the mass balance (in %) was calculated by taking into account the amount of mycotoxin (mg) in the rejected fractions and in the final cleaned maize, with respect to the amount of mycotoxin (mg) in the incoming product (unprocessed maize) according to the formula [1].
Mass   balance   ( % ) = mycotoxin   amount   in   all   collected   fractions / mycotoxin   amount   in   starting   maize   unprocessed   maize   ×   100
The information about the mass loss (%) was provided taking in account the technical specifications of the plants and from previous measurements carried out during the processing, before starting with the trials.

Author Contributions

Conceptualization, M.P., M.G. and K.S.; Data curation, M.P. and K.S.; Funding acquisition, A.F.L.; Investigation, M.P., V.L., A.D.G., S.C., V.M.T.L., B.C., A.V., M.R., M.G. and K.S.; Project administration, M.P., A.F.L., M.G. and K.S.; Supervision, M.G. and K.S.; Validation, V.L., A.D.G., S.C., V.M.T.L., B.C., A.V. and M.R.; Visualization, M.P. and K.S.; Writing—original draft, M.P. and K.S.; Writing—review and editing, M.P., A.F.L., V.L., A.D.G., S.C., V.M.T.L., B.C., A.V., M.R. and K.S. All authors have read and agreed to the published version of the manuscript.

Funding

This research has been supported in part by the MYCOKEY project “Integrated and innovative key actions for mycotoxin management in the food and feed chain” (H2020-Grant Agreement No. 678781) funded under: SOCIETAL CHALLENGES—Food security, sustainable agriculture and forestry, marine, maritime and inland water research, and the bioeconomy.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data sharing not applicable.

Acknowledgments

The authors thank Giuseppe Panzarini (CNR-ISPA, Italy) for the valuable technical support and to Marinella Cavallo (CNR-ISPA) and Simonetta Martena (CNR-ISPA) for their skilled administrative support provided during the realization of this work.

Conflicts of Interest

All authors declare no conflict of interest.

References

  1. Bottalico, A.; Perrone, G. Toxigenic Fusarium species and Mycotoxins Associated with Head Blight in Small-Grain Cereals in Europe. Eur. J. Plant Pathol. 2002, 108, 611–624. [Google Scholar] [CrossRef]
  2. Ji, F.; He, D.; Olaniran, A.O.; Mokoena, M.P.; Xu, J.; Shi, J. Occurrence, toxicity, production and detection of Fusarium mycotoxin: A review. Food Prod. Process. Nutr. 2019, 1, 6. [Google Scholar] [CrossRef] [Green Version]
  3. Torres, A.M.; Palacios, S.A.; Yerkovich, N.; Palazzini, J.M.; Battilani, P.; Leslie, J.F.; Logrieco, A.F.; Chulze, S.N. Fusarium head blight and mycotoxins in wheat: Prevention and control strategies across the food chain. World Mycotoxin J. 2019, 12, 333–355. [Google Scholar] [CrossRef]
  4. Munkvold, G.P.; Proctor, R.H.; Moretti, A. Mycotoxin Production in Fusarium According to Contemporary Species Concepts. Annu. Rev. Phytopathol. 2021, 59, 373–402. [Google Scholar] [CrossRef] [PubMed]
  5. Bulder, A.S.; DiNovi, M.; Kpodo, K.A.; Leblanc, J.-C.; Resnik, S.; Shephard, G.S.; Slob, W.; Walker, R.; Wolterink, G. Deoxynivalenol (addendum). In Safety Evaluation of Certain Contaminants in Food (Prepared by the Seventy-Second Meeting of the Joint FAO/WHO Expert Committee on Food Additives, JECFA); WHO Food Additives Series No. 63; FAO JECFA Monographs 8; World Health Organization: Geneva, Switzerland, 2011; pp. 317–485. [Google Scholar]
  6. Eriksen, G.S.; Pennington, J.; Schlatter, J.; Alexander, J.; Thuvander, A. Zearalenone. In Safety Evaluation of Certain Food Additives and Contaminants (Prepared by the Fifty-Third Meeting of the Joint FAO/WHO Expert Committee on Food Additives, JECFA); WHO Food Additives Series No. 44; IPCS—International Programme on Chemical Safety; WHO: Geneva, Switzerland, 2000. [Google Scholar]
  7. Riley, R.T.; Edwards, S.G.; Aidoo, K.; Alexander, J.; Bolger, M.; Boon, P.E.; Cressey, P.; Doerge, D.R.; Edler, L.; Miller, J.D.; et al. Fumonisins (addendum). In Safety Evaluation of Certain Contaminants in Food (Prepared by Eighty-Third Meeting of the Joint FAO/WHO Expert Committee on Food Additives, JECFA); WHO Food Additives Series No. 74; FAO JECFA Monographs 19 bis; World Health Organization: Geneva, Switzerland, 2018; pp. 415–574. [Google Scholar]
  8. EFSA CONTAM Panel (EFSA Panel on Contaminants in the Food Chain); Knutsen, H.K.; Alexander, J.; Barregard, L.; Bignami, M.; Bruschweiler, B.; Ceccatelli, S.; Cottrill, B.; Dinovi, M.; Grasl-Kraupp, B.; et al. Scientific Opinion on the risks to human and animal health related to the presence of deoxynivalenol and its acetylated and modified forms in food and feed. EFSA J. 2017, 15, e04718. [Google Scholar] [CrossRef] [PubMed]
  9. EFSA CONTAM Panel (EFSA Panel on Contaminants in the Food Chain); Knutsen, H.K.; Alexander, J.; Barregard, L.; Bignami, M.; Bruschweiler, B.; Ceccatelli, S.; Cottrill, B.; Dinovi, M.; Edler, L.; et al. Scientific opinion on the risks for animal health related to the presence of zearalenone and its modified forms in feed. EFSA J. 2017, 15, e04851. [Google Scholar] [CrossRef] [Green Version]
  10. EFSA CONTAM Panel (EFSA Panel on Contaminants in the Food Chain); Knutsen, H.K.; Alexander, J.; Barregard, L.; Bignami, M.; Bruschweiler, B.; Ceccatelli, S.; Cottrill, B.; Dinovi, M.; Edler, L.; et al. Scientific opinion on the risks for animal health related to the presence of fumonisins, their modified forms and hidden forms in feed. EFSA J. 2018, 16, e05242. [Google Scholar] [CrossRef]
  11. IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. In Some Traditional Herbal Medicines, Some Mycotoxins, Naphthalene and Styrene; IARC Press: Lyon, France, 2002; Volume 82, pp. 301–366. [Google Scholar]
  12. van Egmond, H.P.; Jonker, M.A. Worldwide Regulations for Mycotoxins in Food and Feed in 2003; FAO Food and Nutrition Paper 81; Food and Agriculture Organization of the United Nations: Rome, Italy, 2004. [Google Scholar]
  13. Commission of the European Communities. Commission Regulation (EC) No 1126/2007 of 28 September 2007 amending Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs as regards Fusarium toxins in maize and maize products. Off. J. Eur. Union 2007, 255, 14–17. [Google Scholar]
  14. Commission of the European Communities. Commission Recommendation (2006/576/EC) of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding. Off. J. Eur. Union 2006, 229, 7–9. [Google Scholar]
  15. Commission of the European Communities0. Commission Recommendation (EU) 2016/1319 of 29 July 2016 amending Recommendation 2006/576/EC as regards deoxynivalenol, zearalenone and ochratoxin A in pet food. Off. J. Eur. Union 2016, 208, 58–60. [Google Scholar]
  16. Commission of the European Communities. Commission Recommendation on prevention and reduction of Fusarium toxins in cereals. Off. J. Eur. Union 2006, 234, 35–40. [Google Scholar]
  17. CODEX ALIMENTARIUS CXC 51-2003 (Amended in 2017) “Code of Practice for the Prevention and Reduction of Mycotoxin Contamination in Cereals”. Available online: http://www.fao.org/fao-who-codexalimentarius/sh-proxy/en/?lnk=1&url=https%253A%252F%252Fworkspace.fao.org%252Fsites%252Fcodex%252FStandards%252FCXC%2B51-2003%252FCXC_051e.pdf (accessed on 29 July 2022).
  18. Reyneri, A.; Bruno, G.; D’Egidio, M.G.; Balconi, C. Guidelines for the Control of Mycotoxins in Maize and Wheat, 2015, MIPAAF, Ministry of Agriculture, Food and Forestry Policies (In Italian). Available online: https://www.politicheagricole.it/flex/cm/pages/ServeBLOB.php/L/IT/IDPagina/9703 (accessed on 29 July 2022).
  19. Grenier, B.; Loureiro-Bracarense, A.-P.; Leslie, J.F.; Oswald, I.P. Physical and Chemical Methods for mycotoxin decontamination in maize. In Mycotoxin Reduction in Grain Chains; Leslie, J.F., Logrieco, A.F., Eds.; John Wiley and Sons, Inc.: Ames, IA, USA, 2004; pp. 116–129. [Google Scholar]
  20. Karlovsky, P.; Suman, M.; Berthiller, F.; De Meester, J.; Eisenbrand, G.; Perrin, I.; Oswald, I.P.; Speijers, G.; Chiodini, A.; Recker, T.; et al. Impact of food processing and detoxification treatments on mycotoxin contamination. Mycotoxin Res. 2016, 32, 179–205. [Google Scholar] [CrossRef] [Green Version]
  21. Peng, W.X.; Marchal, J.L.M.; van der Poel, A.F.B. Strategies to prevent and reduce mycotoxins for compound feed manufacturing. Anim. Feed Sci. Technol. 2018, 237, 129–153. [Google Scholar] [CrossRef]
  22. Neme, K.; Mohammed, A. Mycotoxin occurrence in grains and the role of postharvest management as a mitigation strategies. A review. Food Control 2017, 78, 412–425. [Google Scholar] [CrossRef]
  23. Colovic, R.; Puvaca, N.; Cheli, F.; Avantaggiato, G.; Greco, D.; Duragic, O.; Kos, J.; Pinotti, L. Decontamination of Mycotoxin-Contaminated Feedstuffs and Compound Feed. Toxins 2019, 11, 617. [Google Scholar] [CrossRef] [Green Version]
  24. Odjo, S.; Alakonya, A.E.; Rosales-Nolasco, A.; Molina, A.L.; Munoz, C.; Palacios-Rojas, N. Occurrence and postharvest strategies to help mitigate aflatoxins and fumonisins in maize and their co-exposure to consumers in Mexico and Central America. Food Control 2022, 138, 108968. [Google Scholar] [CrossRef]
  25. Pinton, P.; Suman, M.; Buck, N.; Dellafiora, L.; De Meester, J.; Stadler, D.; Rito, E. Practical Guidance to Mitigation of Mycotoxins during Food Processing; ILSI Europe Report Series 2019; ILSI Europe: Brussels, Belgium, 2019; Available online: https://ilsi.eu/publication/practical-guidance-to-mitigation-of-mycotoxins-during-food-processing (accessed on 29 July 2022)ISBN 9789078637455.
  26. Nada, S.; Nikola, T.; Bozidar, U.; Ilija, D.; Andreja, R. Prevention and practical strategies to control mycotoxins in the wheat and maize chain. Food Control 2022, 136, 108855. [Google Scholar] [CrossRef]
  27. Hoffmans, Y.; Schaarschmidt, S.; Fauhl-Hassek, C.; van der Fels-Klerx, H.J. Factors during Production of Cereal-Derived Feed That Influence Mycotoxin Contents. Toxins 2022, 14, 301. [Google Scholar] [CrossRef]
  28. Shi, H.; Stroshine, R.; Ileleji, K. Aflatoxin reduction in corn by cleaning and sorting. In Proceedings of the American Society of Agricultural and Biological Engineers, Annual International Meeting 2014, Montreal, QC, Canada, 13–16 July 2014; Volume 1, pp. 311–321. [Google Scholar]
  29. Shi, H.; Stroshine, R.L.; Ileleji, K. Differences in kernel shape, size, and density between healthy kernels and mold discolored kernels and their relationship to reduction in aflatoxin levels in a sample of shelled corn. Appl. Eng. Agric. 2017, 33, 421–431. [Google Scholar] [CrossRef]
  30. Ngure, F.M.; Ngure, C.; Achieng, G.; Munga, F.; Moran, Z.; Stafstrom, W.; Nelson, R.J. Mycotoxins contamination of market maize and the potential of density sorting in reducing exposure in unregulated food systems in Kenya. World Mycotoxin. J. 2020, 14, 165–178. [Google Scholar] [CrossRef]
  31. Sydenham, E.W.; Van der Westhuizen, L.; Stockenström, S.; Shephard, G.S.; Thiel, P.G. Fumonisin-contaminated maize: Physical treatment for the partial decontamination of bulk shipments. Food Addit. Contam. 1994, 11, 25–32. [Google Scholar] [CrossRef] [PubMed]
  32. Aoun, M.; Stafstroma, W.; Priest, P.; John Fuchs, J.; Windhamd, G.L.; Williams, P.W.; Nelson, R.J. Low-cost grain sorting technologies to reduce mycotoxin contamination in maize and groundnut. Food Control 2020, 118, 107363. [Google Scholar] [CrossRef] [PubMed]
  33. Carmack, W.J.; Clark, A.J.; Dong, Y.; van Sanford, D.A. Mass Selection for Reduced Deoxynivalenol Concentration Using an Optical Sorter in SRW Wheat. Agronomy 2019, 9, 816. [Google Scholar] [CrossRef] [Green Version]
  34. Nagy, E.; Korzenszky, P.; Sembery, P. The role of color sorting machine in reducing food safety risks. Potravinarstvo 2016, 10, 354–358. [Google Scholar] [CrossRef]
  35. Stasiewicz, M.J.; Falade, T.D.O.; Mutuma, M.; Mutiga, S.K.; Harvey, J.J.W.; Fox, G.; Pearson, T.C.; Muthomi, J.W.; Nelson, R.J. Multi-spectral kernel sorting to reduce aflatoxins and fumonisins in Kenyan maize. Food Control 2017, 78, 203–214. [Google Scholar] [CrossRef] [Green Version]
  36. Schaarschmidt, S.; Fauhl-Hassek, C. The Fate of Mycotoxins During the Processing of Wheat for Human Consumption. Compr. Rev. Food Sci. Food Saf. 2018, 17, 556–593. [Google Scholar] [CrossRef] [Green Version]
  37. Visconti, A.; Haidukowski, M.; Pascale, M.; Silvestri, M. Reduction of deoxynivalenol during durum wheat processing and spaghetti cooking. Toxicol. Lett. 2004, 153, 181–189. [Google Scholar] [CrossRef]
  38. Pascale, M.; Haidukowski, M.; Lattanzio, V.M.T.; Silvestri, M.; Ranieri, R.; Visconti, A. Distribution of T-2 and HT-2 Toxins in Milling Fractions of Durum Wheat. J. Food Prot. 2011, 74, 1700–1707. [Google Scholar] [CrossRef] [Green Version]
  39. Tibola, C.S.; Fernandes, J.M.C.; Guarienti, E.M. Effect of cleaning, sorting and milling processes in wheat mycotoxin content. Food Control 2016, 60, 174–179. [Google Scholar] [CrossRef] [Green Version]
  40. Brodal, G.; Aamot, H.U.; Almvik, M.; Hofgaard, I.S. Removal of Small Kernels Reduces the Content of Fusarium Mycotoxins in Oat Grain. Toxins 2020, 12, 346. [Google Scholar] [CrossRef]
  41. Schaarschmidt, S.; Fauhl-Hassek, C. The fate of mycotoxins during the primary food processing of maize. Food Control 2021, 121, 107651. [Google Scholar] [CrossRef]
  42. Fandohan, P.; Zoumenou, D.; Hounhouigan, D.J.; Marasas, W.F.O.; Wingfield, M.J.; Hell, K. Fate of aflatoxins and fumonisins during the processing of maize into food products in Benin. Int. J. Food Microbiol. 2005, 98, 249–259. [Google Scholar] [CrossRef]
  43. Matumba, L.; Van Poucke, C.; Ediage, E.N.; Jacobs, B.; De Saeger, S. Effectiveness of hand sorting, flotation/washing, dehulling and combinations thereof on the decontamination of mycotoxin-contaminated white maize. Food Addit. Contam. Part A 2015, 32, 960–969. [Google Scholar] [CrossRef]
  44. van der Westhuizen, L.; Shephard, G.S.; Rheeder, J.P.; Burger, H.M.; Gelderblom, W.C.A.; Wild, C.P.; Gong, Y.Y. Optimising sorting and washing of home-grown maize to reduce fumonisin contamination under laboratory-controlled conditions. Food Control 2011, 22, 396–400. [Google Scholar] [CrossRef]
  45. Pacin, A.M.; Resnik, S.L. Reduction of mycotoxin contamination by segregation with sieves prior to maize milling. In Novel Technologies in Food Science: Their Impact on Products, Consumer Trends and the Environment; McElhatton, A., do Amaral Sobral, P.J., Eds.; Springer: New York, NY, USA, 2012; pp. 219–234. [Google Scholar]
  46. Yoder, A.; Tokach, M.D.; DeRouchey, J.M.; Paulk, C.B. Cleaning reduces mycotoxin contamination in corn. Kans. Agric. Exp. Stn. Res. Rep. 2017, 3, 50. [Google Scholar] [CrossRef] [Green Version]
  47. Yoder, A.D.; Stark, C.R.; DeRouchey, J.M.; Tokach, M.D.; Jones, C.K. Mechanically cleaning corn reduces fumonisin concentration. J. Anim. Sci. 2018, 96 (Suppl. 2), 87–88. [Google Scholar] [CrossRef]
  48. Pearson, T.C.; Wicklow, D.T.; Pasikatan, M.C. Reduction of aflatoxin and fumonisin contamination in yellow corn by high-speed dual-wavelength sorting. Cereal Chem. 2004, 81, 490–498. [Google Scholar] [CrossRef] [Green Version]
  49. Pearson, T.C.; Wicklow, D.T.; Brabec, D.L. Characteristics and sorting of white food corn contaminated with mycotoxins. Appl. Eng. Agric. 2010, 26, 109–113. [Google Scholar] [CrossRef] [Green Version]
  50. Schollenberger, M.; Müller, H.M.; Rüfle, M.; Suchy, S.; Drochner, W. Redistribution of 16 Fusarium toxins during commercial dry milling of maize. Cereal Chem. J. 2008, 85, 557–560. [Google Scholar] [CrossRef]
  51. Scarpino, V.; Vanara, F.; Reyneri, A.; Blandino, M. Fate of moniliformin during different large-scale maize dry-milling processes. LWT-Food Sci. Technol. 2020, 123, 109098. [Google Scholar] [CrossRef]
  52. Pietri, A.; Zanetti, M.; Bertuzzi, T. Distribution of aflatoxins and fumonisins in dry-milled maize fractions. Food Addit. Contam. Part A 2009, 26, 372–380. [Google Scholar] [CrossRef] [PubMed]
  53. Vanara, F.; Scarpino, V.; Blandino, M. Fumonisin Distribution in Maize Dry-Milling Products and By-Products: Impact of Two Industrial Degermination Systems. Toxins 2018, 10, 357. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  54. Pascale, M.; Logrieco, A.F.; Graeber, M.; Hirschberger, M.; Reichel, M.; Lippolis, V.; De Girolamo, A.; Lattanzio, V.M.T.; Slettengren, K. Aflatoxin Reduction in Maize by Industrial-Scale Cleaning Solutions. Toxins 2020, 12, 331. [Google Scholar] [CrossRef] [PubMed]
  55. Cheli, F.; Pinotti, L.; Rossi, L.; Dell’Orto, V. Effect of milling procedures on mycotoxin distribution in wheat fractions: A review. LWT-Food Sci. Technol. 2013, 54, 307–314. [Google Scholar] [CrossRef]
  56. Delwiche, S.R. High-speed optical sorting of soft wheat for reduction of deoxynivalenol. Plant Dis. 2005, 89, 1214–1219. [Google Scholar] [CrossRef] [Green Version]
  57. Saito, S.; Ishibashi, J.; Miyamoto, T.; Tateishi, Y.; Ito, T.; Hara, M.; Kawano, M.; Nakajima, T.; Yoshida, M.; Kawamura, T.; et al. Reduction of Wheat DON and NIV Concentrations with Optical Sorters. Trans. ASAE Am. Soc. Agric. Eng. 2009, 52, 859–866. [Google Scholar] [CrossRef]
  58. Generotti, S.; Cirlini, M.; Dall’Asta, C.; Suman, M. Influence of the industrial process from caryopsis to cornmeal semolina on levels of fumonisins and their masked forms. Food Control 2015, 48, 170–174. [Google Scholar] [CrossRef]
  59. European Commission. Commission Regulation (EC) No. 401/2006, of 23 February 2006 laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in foodstuffs. Off. J. Eur. Union 2006, 70, 12–34. [Google Scholar]
  60. Ciasca, B.; De Saeger, S.; De Boevre, M.; Reichel, M.; Pascale, M.; Logrieco, A.F.; Lattanzio, V.M.T. Mycotoxin Analysis of Grain via Dust Sampling: Review, Recent Advances and the Way Forward: The Contribution of the MycoKey Project. Toxins 2022, 14, 381. [Google Scholar] [CrossRef]
  61. MacDonald, S.J.; Chan, D.; Brereton, P.; Damant, A.; Wood, R. Determination of Deoxynivalenol in Cereals and Cereal Products by Immunoaffinity Column Cleanup with Liquid Chromatography: Interlaboratory Study. J. AOAC Int. 2005, 88, 1197–1204. [Google Scholar] [CrossRef] [Green Version]
  62. MacDonald, S.; Anderson, S.; Brereton, P.; Wood, R.; Damant, A. Determination of zearalenone in barley, maize and wheat flour, polenta, and maize-based baby food by immunoaffinity column cleanup with liquid chromatography: Interlaboratory study. J. AOAC Int. 2005, 88, 1733–1740. [Google Scholar] [CrossRef] [Green Version]
  63. Visconti, A.; Solfrizzo, M.; De Girolamo, A. Determination of Fumonisins B1 and B2 in Corn and Corn Flakes by Liquid Chromatography with Immunoaffinity Column Cleanup: Collaborative Study. J. AOAC Int. 2001, 84, 1828–1837. [Google Scholar] [CrossRef] [Green Version]
  64. De Girolamo, A.; Pascale, M.; Visconti, A. Comparison of methods and optimisation of the analysis of fumonisins B1 and B2 in masa flour, an alkaline cooked corn product. Food Addit. Contam. Part A 2011, 28, 667–675. [Google Scholar] [CrossRef]
Figure 1. Content (%) of deoxynivalenol (DON), zearalenone (ZEA) and total fumonisins (FBs) in rejected fractions from separator (2, small, broken and fine material and 3, coarse and fine material), from aspirator (4, dust and husks particles), from concentrator (5, low density kernels), from optical sorter (6, coloured and discoloured and defective maize kernels) of batch A (French maize) and batch B (Spanish maize). Each bar corresponds to the average content of mycotoxin in each batch (two replicates) with respect to the relevant content in uncleaned maize + standard deviation (fraction 1, incoming material).
Figure 1. Content (%) of deoxynivalenol (DON), zearalenone (ZEA) and total fumonisins (FBs) in rejected fractions from separator (2, small, broken and fine material and 3, coarse and fine material), from aspirator (4, dust and husks particles), from concentrator (5, low density kernels), from optical sorter (6, coloured and discoloured and defective maize kernels) of batch A (French maize) and batch B (Spanish maize). Each bar corresponds to the average content of mycotoxin in each batch (two replicates) with respect to the relevant content in uncleaned maize + standard deviation (fraction 1, incoming material).
Toxins 14 00728 g001
Figure 2. Scheme of the industrial cleaning line and sampling points (numbered) for study 1. Fractions: 1, slightly pre-cleaned maize; 2, coloured/discoloured and defective maize kernels from sorter; 3, cleaned maize. Fractions 1, 2, 3: dynamic sampling.
Figure 2. Scheme of the industrial cleaning line and sampling points (numbered) for study 1. Fractions: 1, slightly pre-cleaned maize; 2, coloured/discoloured and defective maize kernels from sorter; 3, cleaned maize. Fractions 1, 2, 3: dynamic sampling.
Toxins 14 00728 g002
Figure 3. Scheme of the industrial cleaning line and sampling points (numbered) for study 2. Fractions: 1, unprocessed maize: 2, small, broken and fine material from separator; 3, coarse and fine material from separator; 4, dust and husks particles from aspirator; 5, lighter maize fractions from concentrator; 6, coloured/discoloured and defective maize kernels from sorter; 7, cleaned maize. All fractions were dynamically sampled.
Figure 3. Scheme of the industrial cleaning line and sampling points (numbered) for study 2. Fractions: 1, unprocessed maize: 2, small, broken and fine material from separator; 3, coarse and fine material from separator; 4, dust and husks particles from aspirator; 5, lighter maize fractions from concentrator; 6, coloured/discoloured and defective maize kernels from sorter; 7, cleaned maize. All fractions were dynamically sampled.
Toxins 14 00728 g003
Table 1. Average yields (%) of maize-cleaning sampled fractions.
Table 1. Average yields (%) of maize-cleaning sampled fractions.
Maize-Cleaning FractionStudy 1Study 2
Sampled
Fraction
Yield (%)
Batches A–C
Sampled
Fraction
Yield (%)
Batches A1, A2
Yield (%)
Batches B1, B2
Unprocessed raw maize a11001100100
Rejected fraction from separator b -23.02.0
Rejected fraction from separator c -30.90.9
Rejected fraction from aspirator d -40.50.5
Rejected fraction from concentrator e -51.50.5
Rejected fraction from optical sorter f25.060.50.1
Cleaned maize395.0793.696.0
a in study 1 the incoming maize was slightly pre-cleaned by mechanical sorting; b small, broken and fine materials; c coarse and fine materials; d dust and husks particles; e low density maize kernels; f coloured/discoloured and defective maize kernels.
Table 2. Effect of industrial-scale sorting by the SORTEX A5 optical sorter on deoxynivalenol (DON), zearalenone (ZEA) and total fumonisins (FBs) content in sampled fractions.
Table 2. Effect of industrial-scale sorting by the SORTEX A5 optical sorter on deoxynivalenol (DON), zearalenone (ZEA) and total fumonisins (FBs) content in sampled fractions.
BatchSampled Fraction 1DON
(µg/kg)
DON Reduction (%)ZEA
(µg/kg)
ZEA Reduction (%)FBs 2
(µg/kg)
FBs 2 Reduction (%)
A111,13063269078568027
2108,54040,31022,850
341005804150
B117,40067446087654028
2168,25018,70022,320
357905904690
C132004466067252027
227,62010,0607860
317802201830
1 Fraction 1 input optical sorter (slightly pre-cleaned by mechanical sorting); fraction 2: rejected fraction from optical sorter (coloured and discoloured and defective maize kernels); fraction 3: cleaned maize (end product); 2 sum of fumonisin B1 and fumonisin B2.
Table 3. Effect of industrial-scale cleaning line (separator-aspirator-concentrator-optical sorter) on deoxynivalenol (DON), zearalenone (ZEA) and total fumonisins (FBs) content in sampled maize fractions from batch A (French maize) and batch B (Spanish maize). Two replicates per batch were carried out, i.e., A1, A2, B1 and B2.
Table 3. Effect of industrial-scale cleaning line (separator-aspirator-concentrator-optical sorter) on deoxynivalenol (DON), zearalenone (ZEA) and total fumonisins (FBs) content in sampled maize fractions from batch A (French maize) and batch B (Spanish maize). Two replicates per batch were carried out, i.e., A1, A2, B1 and B2.
BatchSampled Fraction 1DON
(µg/kg)
DON Reduction (%)ZEA
(µg/kg)
ZEA Reduction (%)FBs 2
(µg/kg)
FBs 2 Reduction (%)
A11250365080170554
29401607640
3126026514,180
413502708100
5260054031,760
65550903640
716010780
A21220524075176534
26801706830
3149023019,720
4130013017,305
5156035028,890
625504905620
7105101160
B11350435582174067
210801154870
3424055048,250
410,680140535,575
5576076052,530
612,2807508240
720010580
B21330485080173550
21030554740
3497056044,980
4990018040,670
5582025039,990
611,2307457400
717010860
1 Fraction 1: unprocessed maize (incoming material); Fraction 2: rejected fraction from separator (small, broken and fine material); Fraction 3: rejected fraction from separator (coarse and fine material); Fraction 4: rejected fractions from aspirator (dust and husks particles); Fraction 5: rejected fraction from concentrator (low density kernels); Fraction 6: rejected fraction from optical sorter (coloured and discoloured and defective maize kernels); Fraction 7: cleaned maize (end product); 2 sum of fumonisin B1 and fumonisin B2.
Table 4. Mass balance (%) of deoxynivalenol (DON), zearalenone (ZEA) and total fumonisins (FBs) in studies 1 and 2.
Table 4. Mass balance (%) of deoxynivalenol (DON), zearalenone (ZEA) and total fumonisins (FBs) in studies 1 and 2.
StudyBatchDON
(%)
ZEA
(%)
FBs 1
(%)
1A849590
B808785
C9610885
2A11055395
A27962114
B1994388
B29637100
1 sum of FB1 and FB2.
Table 5. Number of incremental samples of sampled fractions and weight of the aggregate samples, according to the Commission Regulation (EU) No 401/2006.
Table 5. Number of incremental samples of sampled fractions and weight of the aggregate samples, according to the Commission Regulation (EU) No 401/2006.
StudySampled
Fraction
Number of Incremental SamplesAggregate Sample Weight (kg)
1 11, 310010–14
2101–2
2 21, 7606–10
2101–2
3, 4, 5, 651–2
1 Batches of 25 tons. Fractions: 1, unprocessed maize; 2, foreign bodies and kernels with visual defects from sorter; 3, cleaned maize. 2 Batches of 17 tons. Fractions: 1, unprocessed maize: 2, small, broken and fine material from separator; 3, coarse and fine material from separator; 4, dust and husks particles from aspirator; 5, lighter maize fractions from concentrator; 6, maize kernels with visual signs of contamination; 7, cleaned maize.
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Pascale, M.; Logrieco, A.F.; Lippolis, V.; De Girolamo, A.; Cervellieri, S.; Lattanzio, V.M.T.; Ciasca, B.; Vega, A.; Reichel, M.; Graeber, M.; et al. Industrial-Scale Cleaning Solutions for the Reduction of Fusarium Toxins in Maize. Toxins 2022, 14, 728. https://doi.org/10.3390/toxins14110728

AMA Style

Pascale M, Logrieco AF, Lippolis V, De Girolamo A, Cervellieri S, Lattanzio VMT, Ciasca B, Vega A, Reichel M, Graeber M, et al. Industrial-Scale Cleaning Solutions for the Reduction of Fusarium Toxins in Maize. Toxins. 2022; 14(11):728. https://doi.org/10.3390/toxins14110728

Chicago/Turabian Style

Pascale, Michelangelo, Antonio F. Logrieco, Vincenzo Lippolis, Annalisa De Girolamo, Salvatore Cervellieri, Veronica M. T. Lattanzio, Biancamaria Ciasca, Anna Vega, Mareike Reichel, Matthias Graeber, and et al. 2022. "Industrial-Scale Cleaning Solutions for the Reduction of Fusarium Toxins in Maize" Toxins 14, no. 11: 728. https://doi.org/10.3390/toxins14110728

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop