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Toxins 2018, 10(7), 276; https://doi.org/10.3390/toxins10070276

Low Levels of Chito-Oligosaccharides Are Not Effective in Reducing Deoxynivalenol Toxicity in Swine Jejunal Explants

1
Laboratory of Animal Pathology, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná 86057-970, Brazil
2
Academic Department of Food, Universidade Tecnológica Federal do Paraná, Avenida dos Pioneiros, 3131, Londrina, Paraná 86036-370, Brazil
*
Author to whom correspondence should be addressed.
Received: 29 May 2018 / Revised: 22 June 2018 / Accepted: 26 June 2018 / Published: 4 July 2018
(This article belongs to the Special Issue Effects of Mycotoxins on the Intestine)
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Abstract

Deoxynivalenol (DON) is a mycotoxin that affects the intestinal morphology of animals, impairing nutrient intake and growth. On the other hand, dietary supplementation with functional oligosaccharides as chito-oligosaccharides (COS) has shown positive effects on the intestinal health of piglets. Therefore, the objective of the present study was to evaluate the effect of low doses of COS in preventing DON-induced intestinal histological changes, using a swine jejunal explant technique. The intestinal explants were incubated at 37 °C in culture medium for 4 h and exposed to the following treatments: (a) control (only culture medium), (b) DON (10 µM), (c) 25COS (0.025 mg·mL−1 of COS); (d) 50COS (0.05 mg·mL−1 of COS); (e) 25COS plus DON (25COS + DON); (f) 50COS plus DON (50COS + DON). Explants exposed to COS presented intestinal morphology similar to control samples. DON induced a significant decrease in the histological score as a consequence of moderate to severe histological changes (apical necrosis, villi atrophy, and fusion) and a significant decrease in morphometric parameters (villi height, crypt depth, villi height:crypt depth ratio, and goblet cells density). The intestinal morphology of samples exposed to COS + DON remained similar to DON treatment. In conclusion, low levels of COS did not counteract DON-induced intestinal lesions. View Full-Text
Keywords: functional oligosaccharides; mycotoxins; swine; explant technique; intestinal morphology; goblet cells functional oligosaccharides; mycotoxins; swine; explant technique; intestinal morphology; goblet cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Gerez, J.; Buck, L.; Marutani, V.H.; Calliari, C.M.; Bracarense, A.P. Low Levels of Chito-Oligosaccharides Are Not Effective in Reducing Deoxynivalenol Toxicity in Swine Jejunal Explants. Toxins 2018, 10, 276.

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