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Nutrients 2017, 9(7), 787;

Iron Bioavailability Studies of the First Generation of Iron-Biofortified Beans Released in Rwanda

USDA-ARS Robert Holley Center for Agriculture and Health, Ithaca, NY 14853, USA
Division of Nutritional Sciences, 220 Savage Hall, Cornell University, Ithaca, NY 14853, USA
International Center for Tropical Agriculture (CIAT), Regional Office for Africa, P.O. Box 823-00621, Nairobi 00100, Kenya
International Center for Tropical Agriculture (CIAT), Km 17, Recta Cali–Palmira CP 763537, Apartado Aéreo 6713, Cali, Colombia
Author to whom correspondence should be addressed.
Received: 22 June 2017 / Revised: 12 July 2017 / Accepted: 17 July 2017 / Published: 21 July 2017
(This article belongs to the Special Issue Fe Deficiency, Dietary Bioavailbility and Absorption)
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This paper represents a series of in vitro iron (Fe) bioavailability experiments, Fe content analysis and polyphenolic profile of the first generation of Fe biofortified beans (Phaseolus vulgaris) selected for human trials in Rwanda and released to farmers of that region. The objective of the present study was to demonstrate how the Caco-2 cell bioassay for Fe bioavailability can be utilized to assess the nutritional quality of Fe in such varieties and how they may interact with diets and meal plans of experimental studies. Furthermore, experiments were also conducted to directly compare this in vitro approach with specific human absorption studies of these Fe biofortified beans. The results show that other foods consumed with beans, such as rice, can negatively affect Fe bioavailability whereas potato may enhance the Fe absorption when consumed with beans. The results also suggest that the extrinsic labelling approach to measuring human Fe absorption can be flawed and thus provide misleading information. Overall, the results provide evidence that the Caco-2 cell bioassay represents an effective approach to evaluate the nutritional quality of Fe-biofortified beans, both separate from and within a targeted diet or meal plan. View Full-Text
Keywords: beans; Phaseolus vulgaris; iron; bioavailability; biofortification beans; Phaseolus vulgaris; iron; bioavailability; biofortification

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Glahn, R.; Tako, E.; Hart, J.; Haas, J.; Lung’aho, M.; Beebe, S. Iron Bioavailability Studies of the First Generation of Iron-Biofortified Beans Released in Rwanda. Nutrients 2017, 9, 787.

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