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Hypocholesterolaemic Activity of Lupin Peptides: Investigation on the Crosstalk between Human Enterocytes and Hepatocytes Using a Co-Culture System Including Caco-2 and HepG2 Cells

1
Department of Pharmaceutical Sciences, University of Milan, Milan I-20133, Italy
2
CREA, Food and Nutrition Research Centre, Rome I-00100, Italy
*
Author to whom correspondence should be addressed.
Nutrients 2016, 8(7), 437; https://doi.org/10.3390/nu8070437
Received: 7 June 2016 / Revised: 7 July 2016 / Accepted: 14 July 2016 / Published: 22 July 2016
(This article belongs to the Special Issue Health Benefits of Soybean and other Grain Legumes)
Literature indicates that peptic and tryptic peptides derived from the enzymatic hydrolysis of lupin protein are able to modulate cholesterol metabolism in human hepatic HepG2 cells and that part of these peptides are absorbed in a small intestine model based on differentiated human Caco-2 cells. In this paper, a co-culture system, including Caco-2 and HepG2 cells, was investigated with two objectives: (a) to verify whether cholesterol metabolism in HepG2 cells was modified by the peptides absorption through Caco-2 cells; (b) to investigate how lupin peptides influence cholesterol metabolism in Caco-2 cells. The experiments showed that the absorbed peptides, not only maintained their bioactivity on HepG2 cells, but that this activity was improved by the crosstalk of the two cells systems in co-culture. In addition, lupin peptides showed a positive influence on cholesterol metabolism in Caco-2 cells, decreasing the proprotein convertase subtilisin/kexin type 9 (PCSK9) secretion. View Full-Text
Keywords: bioactive food peptides; cholesterol metabolism; intestinal absorption; LDL receptor; Lupinus; PCSK9 bioactive food peptides; cholesterol metabolism; intestinal absorption; LDL receptor; Lupinus; PCSK9
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Lammi, C.; Zanoni, C.; Ferruzza, S.; Ranaldi, G.; Sambuy, Y.; Arnoldi, A. Hypocholesterolaemic Activity of Lupin Peptides: Investigation on the Crosstalk between Human Enterocytes and Hepatocytes Using a Co-Culture System Including Caco-2 and HepG2 Cells. Nutrients 2016, 8, 437.

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