Anti-Inflammatory Effects of Vitamin D on Human Immune Cells in the Context of Bacterial Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Samples
2.2. Materials
2.3. Cell Culture
2.4. Plasma 25(OH)D Measurement
2.5. Measurement of Cytokines
2.6. Flow Cytometry
2.7. Neutrophil Migration Assay
2.8. Statistics
3. Results
3.1. 1,25(OH)2D3 Suppresses Pro-Inflammatory Cytokines in PBMCs and CD14+ Monocytes
3.2. 1,25(OH)2D3 Enhances CD14 Expression in Stimulated PBMCs
3.3. Plasma 25(OH)D Status Influences the Magnitude of Inflammatory Response
3.4. 1,25(OH)2D3 Regulates Neutrophil Migration
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Hoe, E.; Nathanielsz, J.; Toh, Z.Q.; Spry, L.; Marimla, R.; Balloch, A.; Mulholland, K.; Licciardi, P.V. Anti-Inflammatory Effects of Vitamin D on Human Immune Cells in the Context of Bacterial Infection. Nutrients 2016, 8, 806. https://doi.org/10.3390/nu8120806
Hoe E, Nathanielsz J, Toh ZQ, Spry L, Marimla R, Balloch A, Mulholland K, Licciardi PV. Anti-Inflammatory Effects of Vitamin D on Human Immune Cells in the Context of Bacterial Infection. Nutrients. 2016; 8(12):806. https://doi.org/10.3390/nu8120806
Chicago/Turabian StyleHoe, Edwin, Jordan Nathanielsz, Zheng Quan Toh, Leena Spry, Rachel Marimla, Anne Balloch, Kim Mulholland, and Paul V. Licciardi. 2016. "Anti-Inflammatory Effects of Vitamin D on Human Immune Cells in the Context of Bacterial Infection" Nutrients 8, no. 12: 806. https://doi.org/10.3390/nu8120806
APA StyleHoe, E., Nathanielsz, J., Toh, Z. Q., Spry, L., Marimla, R., Balloch, A., Mulholland, K., & Licciardi, P. V. (2016). Anti-Inflammatory Effects of Vitamin D on Human Immune Cells in the Context of Bacterial Infection. Nutrients, 8(12), 806. https://doi.org/10.3390/nu8120806