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Open AccessArticle

Determination of Free Radical Scavenging, Antioxidative DNA Damage Activities and Phytochemical Components of Active Fractions from Lansium domesticum Corr. Fruit

1
Department of Pharmaceuticals and Natural Products, Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3 Techno Polis, Khlong Luang, Pathum Thani 12120, Thailand
2
The Government Pharmaceutical Organization (GPO), Rama VI Road, Bangkok 10400, Thailand
*
Author to whom correspondence should be addressed.
Nutrients 2015, 7(8), 6852-6873; https://doi.org/10.3390/nu7085312
Received: 29 June 2015 / Accepted: 23 July 2015 / Published: 14 August 2015
(This article belongs to the Special Issue Natural Products for Human Health)
Lansium domesticum Corr. or “long-kong” is one of the most popular fruits in Thailand. Its peel (skin, SK) and seeds (SD) become waste unless recycled or applied for use. This study was undertaken to determine the bioactivity and phytochemical components of L. domesticum (LD) skin and seed extracts. Following various extraction and fractionation procedures, 12 fractions were obtained. All fractions were tested for antioxidant capacity against O2−• and OH. It was found that the peel of L. domesticum fruits exhibited higher O2−• and OH scavenging activity than seeds. High potential antioxidant activity was found in two fractions of 50% ethanol extract of peel followed by ethyl acetate (EA) fractionation (LDSK50-EA) and its aqueous phase (LDSK50-H2O). Therefore, these two active fractions were selected for further studies on their antioxidative activity against DNA damage by hydrogen peroxide (H2O2) in human TK6 cells using comet assay. The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure. The phytochemical analysis illustrated the presence of phenolic substances, mainly scopoletin, rutin, and chlorogenic acid, in these two active fractions. This study generates new information on the biological activity of L. domesticum. It will promote and strengthen the utilization of L. domesticum by-products. View Full-Text
Keywords: Lansium domesticum; long-kong; superoxide (O2); hydroxyl (OH); hydrogen peroxide (H2O2); DNA damage; comet test Lansium domesticum; long-kong; superoxide (O2); hydroxyl (OH); hydrogen peroxide (H2O2); DNA damage; comet test
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Klungsupya, P.; Suthepakul, N.; Muangman, T.; Rerk-Am, U.; Thongdon-A, J. Determination of Free Radical Scavenging, Antioxidative DNA Damage Activities and Phytochemical Components of Active Fractions from Lansium domesticum Corr. Fruit. Nutrients 2015, 7, 6852-6873.

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