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Open AccessArticle

Nrf2-Mediated HO-1 Induction Contributes to Antioxidant Capacity of a Schisandrae Fructus Ethanol Extract in C2C12 Myoblasts

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Blue-Bio Industry Regional Innovation Center and Anti-Aging Research Center, Dongeui University, Busan 614-714, Korea
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Department of Biochemistry, College of Korean Medicine, Dongeui University, Busan 614-052, Korea
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Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Korea
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Department of Biochemistry, College of Medicine, Busan National University, Yangsan 626-870, Korea
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Department of Life Science and Biotechnology, College of Natural Sciences, Dongeui University, Busan 614-714, Korea
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Department of Food and Nutrition, College of Human Ecology, Dongeui University, Busan 614-714, Korea
*
Author to whom correspondence should be addressed.
Nutrients 2014, 6(12), 5667-5678; https://doi.org/10.3390/nu6125667
Received: 8 October 2014 / Revised: 16 November 2014 / Accepted: 24 November 2014 / Published: 8 December 2014
This study was designed to confirm the protective effect of Schisandrae Fructus, which are the dried fruits of Schisandra chinensis (Turcz.) Baill, against oxidative stress-induced cellular damage and to elucidate the underlying mechanisms in C2C12 myoblasts. Preincubating C2C12 cells with a Schisandrae Fructus ethanol extract (SFEE) significantly attenuated hydrogen peroxide (H2O2)-induced inhibition of growth and induced scavenging activity against intracellular reactive oxygen species (ROS) induced by H2O2. SFEE also inhibited comet tail formation and phospho-histone γH2A.X expression, suggesting that it prevents H2O2-induced cellular DNA damage. Furthermore, treating C2C12 cells with SFEE significantly induced heme oxygenase-1 (HO-1) and phosphorylation of nuclear factor-erythroid 2 related factor 2 (Nrf2). However, zinc protoporphyrin IX, a potent inhibitor of HO-1 activity, significantly reversed the protective effects of SFEE against H2O2-induced growth inhibition and ROS generation in C2C12 cells. Additional experiments revealed that the potential of the SFEE to induce HO-1 expression and protect against H2O2-mediated cellular damage was abrogated by transient transfection with Nrf2-specific small interfering RNA, suggesting that the SFEE protected C2C12 cells against oxidative stress-induced injury through the Nrf2/HO-1 pathway. View Full-Text
Keywords: Schisandrae Fructus; oxidative stress; ROS; Nrf2/HO-1 Schisandrae Fructus; oxidative stress; ROS; Nrf2/HO-1
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Kang, J.S.; Han, M.H.; Kim, G.-Y.; Kim, C.M.; Kim, B.W.; Hwang, H.J.; Choi, Y.H. Nrf2-Mediated HO-1 Induction Contributes to Antioxidant Capacity of a Schisandrae Fructus Ethanol Extract in C2C12 Myoblasts. Nutrients 2014, 6, 5667-5678.

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