Electrolyzed Hydrogen Water Alleviates Abdominal Pain through Suppression of Colonic Tissue Inflammation in a Rat Model of Inflammatory Bowel Disease
, Tianliang Huang 1, Mika Shigeta 1, Yuta Ochi 1, Shigeru Kabayama 2, Yasuyoshi Watanabe 3 and Yilong Cui 1,*Round 1
Reviewer 1 Report
This is a well-designed experimental study with translational significance. However there are a few minor issues need to be carefully addressed.
1. In Discussion, please provide limitation of the present study. For example, (1) the pretreatment suggest the anti-inflammatory action, but its therapeutic potential is not tested yet; (2) the underlying molecular mechanisms need to be future investigated.
2. In Discussion, please provide a brief statement on the future research direction including clinical translational research.
Author Response
Reviewer 1 comments
This is a well-designed experimental study with translational significance. However there are a few minor issues need to be carefully addressed.
- In Discussion, please provide limitation of the present study. For example, (1) the pretreatment suggest the anti-inflammatory action, but its therapeutic potential is not tested yet; (2) the underlying molecular mechanisms need to be future investigated.
>>>Responses:
We greatly appreciate the reviewer’s comments that allowed us to improve the quality of the manuscript. According to the reviewer’s comments, we added a paragraph regarding the limitations at the end of the Discussion section. (Page 12, Line 432-441. In red letters)
- In Discussion, please provide a brief statement on the future research direction including clinical translational research.
>>>Responses:
We greatly appreciate the reviewer’s comments. We added a future research direction of EHW in the Discussion section. (Page 12, Line 441-445. In red letters)
Reviewer 2 Report
In general, the study was well-performed and manuscript is well-written. Please explain why the TNBS model was used for the study.
Author Response
Reviewer 2 comments
In general, the study was well-performed and manuscript is well-written. Please explain why the TNBS model was used for the study.
>>>Responses:
We greatly appreciate the reviewer’s comments. To make this point clearer, we added some explanations and relevant references on the background of TNBS model in the Introduction section. (Page 2, Line 79-86. In red letters)
Reviewer 3 Report
An Interesting original article describing how hydrogen water may alleviate pain sensation by suppressing inflammation in a rat IBD model, opening further experimentations also in humans about its possible use in medicine; only minor queries:
A description of currently available therapeutic drugs in Human IBD should be added in the introduction; here an interesting article: doi: 10.1080/03007995.2020.1786681.
Thank You and good luck!
Author Response
Reviewer 3 comments
An Interesting original article describing how hydrogen water may alleviate pain sensation by suppressing inflammation in a rat IBD model, opening further experimentations also in humans about its possible use in medicine; only minor queries:
A description of currently available therapeutic drugs in Human IBD should be added in the introduction; here an interesting article: doi: 10.1080/03007995.2020.1786681.
>>>Responses:
We deeply appreciate the reviewer’s comments. According to the reviewer’s comments, we added some descriptions of available therapeutic approach in IBD in the Introduction section (Page 2, Line 50-54. In red letters)
