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Article

Evaluation of Anti-Cytotoxic and Anti-Genotoxic Effects of Nigella sativa through a Micronucleus Test in BALB/c Mice

1
Facultad de Medicina, Universidad Autónoma de Guadalajara, Zapopan 45129, JAL, Mexico
2
Laboratorio de Evaluación de Genotóxicos, Programa Internacional de Medicina, Universidad Autónoma de Guadalajara, Zapopan 45129, JAL, Mexico
3
Facultad de Medicina, Decanato Ciencias de la Salud, Universidad Autónoma de Guadalajara, Zapopan 45129, JAL, Mexico
*
Author to whom correspondence should be addressed.
Nutrients 2020, 12(5), 1317; https://doi.org/10.3390/nu12051317
Received: 28 February 2020 / Revised: 22 April 2020 / Accepted: 24 April 2020 / Published: 6 May 2020
Nigella sativa (N. sativa) is a medicinal plant used for its therapeutic pharmacological effects such as anti-inflammatory, antioxidant, anticancer, antidiabetic, and immunomodulation. This study explored the anti-cytotoxic and anti-genotoxic effect of N. sativa through a micronucleus test (MNT) of BALB/c mice peripheral blood. Using 6-to-8-week-old healthy male BALB/c mice, four groups were formed: (1) Control (sterile water), single-dose 2 mg/kg/intraperitoneal (i.p); (2) N. sativa oil, 500 mg/kg/24 h/7 days/i.p; (3) Cisplatin (CP), single-dose 2 mg/kg/subcutaneous (s.c); (4) N. sativa + CP with their respective dosage. When evaluating polychromatic erythrocytes (PCE), a biomarker of cytotoxicity, the group treated with N. sativa + CP experienced an increase in the frequency of PCE, which demonstrated the recovery of bone marrow and modulation of cell proliferation. The analysis of micronucleated polychromatic erythrocytes (MNPCE), an acute genotoxicity biomarker, showed similar frequency of MNPCE within the groups except in CP, but, in the N. sativa + CP group, the frequency of MNPCE decreased and then regulated. Finally, the frequency of micronucleated erythrocytes (MNE), a biomarker of genotoxicity, the supplementation of N. sativa oil did not induce genotoxic damage in this model. Thus, we conclude that N. sativa has both cytoprotective, genoprotective effects and modulates cell proliferation in BALB/c mice. View Full-Text
Keywords: Nigella sativa; thymoquinone; cisplatin; micronucleus test; micronuclei; Howell–Jolly bodies; anti-cytotoxicity; anti-genotoxicity; BALB/c mice; bone marrow Nigella sativa; thymoquinone; cisplatin; micronucleus test; micronuclei; Howell–Jolly bodies; anti-cytotoxicity; anti-genotoxicity; BALB/c mice; bone marrow
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MDPI and ACS Style

Franco-Ramos, R.S.; López-Romero, C.A.; Torres-Ortega, H.; Oseguera-Herrera, D.; Lamoreaux-Aguayo, J.P.; Molina-Noyola, D.; Juárez-Vázquez, C.I.; Torres-Bugarín, O. Evaluation of Anti-Cytotoxic and Anti-Genotoxic Effects of Nigella sativa through a Micronucleus Test in BALB/c Mice. Nutrients 2020, 12, 1317. https://doi.org/10.3390/nu12051317

AMA Style

Franco-Ramos RS, López-Romero CA, Torres-Ortega H, Oseguera-Herrera D, Lamoreaux-Aguayo JP, Molina-Noyola D, Juárez-Vázquez CI, Torres-Bugarín O. Evaluation of Anti-Cytotoxic and Anti-Genotoxic Effects of Nigella sativa through a Micronucleus Test in BALB/c Mice. Nutrients. 2020; 12(5):1317. https://doi.org/10.3390/nu12051317

Chicago/Turabian Style

Franco-Ramos, Raúl S., Carlos A. López-Romero, Hugo Torres-Ortega, Darío Oseguera-Herrera, Jose P. Lamoreaux-Aguayo, Daniel Molina-Noyola, Clara I. Juárez-Vázquez, and Olivia Torres-Bugarín. 2020. "Evaluation of Anti-Cytotoxic and Anti-Genotoxic Effects of Nigella sativa through a Micronucleus Test in BALB/c Mice" Nutrients 12, no. 5: 1317. https://doi.org/10.3390/nu12051317

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