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Fluoxetine Mimics the Anorectic Action of Estrogen and Its Regulation of Circadian Feeding in Ovariectomized Female Rats
Open AccessArticle

Estradiol Replacement Improves High-Fat Diet-Induced Obesity by Suppressing the Action of Ghrelin in Ovariectomized Rats

1
Department of Environmental Health, Faculty of Human Life and Environment, Nara Women’s University, Kitauoya Nishimachi, Nara 630-8506, Japan
2
Department of Health and Nutrition, Faculty of Health Science, Kyoto Koka Women’s University, 38 Kadono-cho, Nishikyogoku, Ukyo-ku, Kyoto 615-0882, Japan
*
Author to whom correspondence should be addressed.
Nutrients 2020, 12(4), 907; https://doi.org/10.3390/nu12040907 (registering DOI)
Received: 16 February 2020 / Revised: 17 March 2020 / Accepted: 24 March 2020 / Published: 26 March 2020
(This article belongs to the Special Issue The Relationship between Diet and Hormones)
This study aims to investigate the effects of estradiol replacement on the orexigenic action of ghrelin in ovariectomized (OVX) obese rats fed with a high-fat diet (HFD). Four weeks after OVX at 9 weeks of age, Wistar rats were subcutaneously implanted with either 17β-estradiol (E2) or placebo (Pla) pellets and started on HFD feeding. After 4 weeks, growth hormone-releasing peptide (GHRP)-6, a growth hormone secretagogue receptor (GHSR) agonist injected intraperitoneally, induced changes in HFD intake, and c-Fos-positive neurons in the hypothalamic arcuate nucleus (ARC) were measured in both groups. The ghrelin protein and mRNA levels, as well as GHSR protein in stomach, were analyzed by Western blotting and real-time PCR. HFD increased energy intake and body weight in the Pla group, while it temporarily reduced these in the E2 group. GHRP-6 enhanced HFD intake and activated neurons in the ARC only in the Pla group. Furthermore, gastric ghrelin and GHSR protein levels were lower in the E2 group than in the Pla group, but plasma acyl ghrelin levels were similar in both groups. Our results suggest that E2 replacement improves obesity by inhibiting the orexigenic action of ghrelin via downregulation of ghrelin and its receptor in stomach in HFD-fed OVX rats. View Full-Text
Keywords: estradiol replacement; ghrelin; growth hormone secretagogue receptor; energy intake; high-fat diet-induced obesity; ovariectomized rat estradiol replacement; ghrelin; growth hormone secretagogue receptor; energy intake; high-fat diet-induced obesity; ovariectomized rat
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Yokota-Nakagi, N.; Takahashi, H.; Kawakami, M.; Takamata, A.; Uchida, Y.; Morimoto, K. Estradiol Replacement Improves High-Fat Diet-Induced Obesity by Suppressing the Action of Ghrelin in Ovariectomized Rats. Nutrients 2020, 12, 907.

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