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Article

Polarization of Macrophages in Human Adipose Tissue is Related to the Fatty Acid Spectrum in Membrane Phospholipids

1
Centre for Experimental Medicine, Institute for Clinical and Experimental Medicine, 140-21 Prague, Czech Republic
2
Transplant Surgery Dept., Institute for Clinical and Experimental Medicine, 140-21 Prague, Czech Republic
3
Anaestesiology, Resuscitation and Intensive Care Unit, Military University Hospital, 140-21 Prague, Czech Republic
*
Authors to whom correspondence should be addressed.
Nutrients 2020, 12(1), 8; https://doi.org/10.3390/nu12010008
Received: 12 November 2019 / Revised: 16 December 2019 / Accepted: 17 December 2019 / Published: 18 December 2019
(This article belongs to the Special Issue Diet, Nutrition and Abdominal Obesity)
Residential macrophages in adipose tissue play a pivotal role in the development of inflammation not only within this tissue, but also affect the proinflammatory status of the whole body. Data on human adipose tissue inflammation and the role of macrophages are rather scarce. We previously documented that the proportion of proinflammatory macrophages in human adipose tissue correlates closely with non-HDL cholesterol concentrations. We hypothesized that this is due to the identical influence of diet on both parameters and decided to analyze the fatty acid spectrum in cell membrane phospholipids of the same individuals as a parameter of the diet consumed. Proinflammatory and anti-inflammatory macrophages were isolated from human adipose tissue (n = 43) and determined by flow cytometry as CD14+CD16+CD36high and CD14+CD16−CD163+, respectively. The spectrum of fatty acids in phospholipids in the cell membranes of specimens of the same adipose tissue was analyzed, and the proportion of proinflammatory macrophage increased with the proportions of palmitic and palmitoleic acids. Contrariwise, these macrophages decreased with increasing alpha-linolenic acid, total n-3 fatty acids, n-3/n-6 ratio, and eicosatetraenoic acid. A mirror picture was documented for the proportion of anti-inflammatory macrophages. The dietary score, obtained using a food frequency questionnaire, documented a positive relation to proinflammatory macrophages in individuals who consumed predominantly vegetable fat and fish, and individuals who consumed diets based on animal fat without fish and nut consumption. he present data support our hypothesis that macrophage polarization in human visceral adipose tissue is related to fatty acid metabolism, cell membrane composition, and diet consumed. It is suggested that fatty acid metabolism might participate also in inflammation and the risk of developing cardiovascular disease. View Full-Text
Keywords: macrophages; membrane; inflammation; nutrition; omega-3 fatty acids macrophages; membrane; inflammation; nutrition; omega-3 fatty acids
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MDPI and ACS Style

Poledne, R.; Malinska, H.; Kubatova, H.; Fronek, J.; Thieme, F.; Kauerova, S.; Kralova Lesna, I. Polarization of Macrophages in Human Adipose Tissue is Related to the Fatty Acid Spectrum in Membrane Phospholipids. Nutrients 2020, 12, 8. https://doi.org/10.3390/nu12010008

AMA Style

Poledne R, Malinska H, Kubatova H, Fronek J, Thieme F, Kauerova S, Kralova Lesna I. Polarization of Macrophages in Human Adipose Tissue is Related to the Fatty Acid Spectrum in Membrane Phospholipids. Nutrients. 2020; 12(1):8. https://doi.org/10.3390/nu12010008

Chicago/Turabian Style

Poledne, Rudolf, Hana Malinska, Hana Kubatova, Jiri Fronek, Filip Thieme, Sona Kauerova, and Ivana Kralova Lesna. 2020. "Polarization of Macrophages in Human Adipose Tissue is Related to the Fatty Acid Spectrum in Membrane Phospholipids" Nutrients 12, no. 1: 8. https://doi.org/10.3390/nu12010008

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