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Dietary Bioactive Fatty Acids as Modulators of Immune Function: Implications on Human Health
Open AccessArticle

Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells

1
Research Group on Community Nutrition and Oxidative Stress, University of Balearic Islands & IDISBA, 07122 Palma de Mallorca, Spain
2
CIBEROBN (Physiopathology of Obesity and Nutrition), Instituto de Salud Carlos III, 28029 Madrid, Spain
3
IDISBA (Institut d’Investigació Sanitària de les Illes Balears), Fundació Institut d’Investigació Sanitària de les Illes Balears, 07120 Palma de Mallorca, Spain
4
Department of Nutrition and Dietetics, Faculty of Pharmacy, University of Concepcion, 4070386 Concepcion, Chile
*
Author to whom correspondence should be addressed.
These authors contributed equally to this manuscript.
Nutrients 2020, 12(1), 146; https://doi.org/10.3390/nu12010146
Received: 23 October 2019 / Revised: 28 November 2019 / Accepted: 31 December 2019 / Published: 4 January 2020
(This article belongs to the Special Issue Lipid Metabolism in Inflammation and Immune Function)
The aim of this study was to assess free fatty acids’ (FAs) ex vivo anti-/proinflammatory capabilities and their influence on inflammatory gene expression and H2O2 production by human peripheral blood mononuclear cells (PBMCs). Anthropometric and clinical measurements were performed in 26 participants with metabolic syndrome. Isolated PBMCs were incubated ex vivo for 2 h with several free fatty acids—palmitic, oleic, α-linolenic, γ-linolenic, arachidonic and docosahexaenoic at 50 μM, and lipopolysaccharide (LPS) alone or in combination. H2O2 production and IL6, NFκB, TLR2, TNFα, and COX-2 gene expressions were determined. Palmitic, γ-linolenic, and arachidonic acids showed minor effects on inflammatory gene expression, whereas oleic, α-linolenic, and docosahexaenoic acids reduced proinflammatory gene expression in LPS-stimulated PBMCs. Arachidonic and α-linolenic acids treatment enhanced LPS-stimulated H2O2 production by PBMCs, while palmitic, oleic, γ-linolenic, and docosahexaenoic acids did not exert significant effects. Oleic, α-linolenic, and docosahexaenoic acids induced anti-inflammatory responses in PBMCs. Arachidonic and α-linolenic acids enhanced the oxidative status of LPS-stimulated PBMCs. In conclusion, PBMC ex vivo assays are useful to assess the anti-/proinflammatory and redox-modulatory effects of fatty acids or other food bioactive compounds. View Full-Text
Keywords: PBMC; fatty acids; LPS; gene expression; ROS production PBMC; fatty acids; LPS; gene expression; ROS production
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Sureda, A.; Martorell, M.; Bibiloni, M.M.; Bouzas, C.; Gallardo-Alfaro, L.; Mateos, D.; Capó, X.; Tur, J.A.; Pons, A. Effect of Free Fatty Acids on Inflammatory Gene Expression and Hydrogen Peroxide Production by Ex Vivo Blood Mononuclear Cells. Nutrients 2020, 12, 146.

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