Effects of Macronutrients on the In Vitro Production of ClpB, a Bacterial Mimetic Protein of α-MSH and Its Possible Role in Satiety Signaling
by
Manon Dominique 1,2,3, Jonathan Breton 2,3,4
, Charlène Guérin 2,3, Christine Bole-Feysot 2,3, Grégory Lambert 1, Pierre Déchelotte 1,2,3,4 and Sergueï Fetissov 1,2,3,5,*
Manon Dominique 1,2,3, Jonathan Breton 2,3,4, Charlène Guérin 2,3, Christine Bole-Feysot 2,3, Grégory Lambert 1, Pierre Déchelotte 1,2,3,4 and Sergueï Fetissov 1,2,3,5,*
1
TargEDys SA, Faculty of Medicine and Pharmacy, University of Rouen Normandy, 22, Boulevard Gambetta, Cedex 01, 76183 Rouen, France
2
Nutrition, Gut and Brain Laboratory, Inserm UMR1073, University of Rouen Normandy, 76183 Rouen, France
3
Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen Normandy, 76183 Rouen, France
4
Rouen University Hospital, CHU Charles Nicolle, 76183 Rouen, France
5
Laboratory of Neuronal and Neuroendocrine Differentiation and Communication, Inserm UMR1239, University of Rouen Normandy, 76130 Mont-Saint-Aignan, France
*
Author to whom correspondence should be addressed.
Nutrients 2019, 11(9), 2115; https://doi.org/10.3390/nu11092115
Received: 18 June 2019 / Revised: 26 August 2019 / Accepted: 1 September 2019 / Published: 5 September 2019
Gut microbiota can influence the feeding behavior of the host, but the underlying mechanisms are unknown. Recently, caseinolytic protease B (ClpB), a disaggregation chaperon protein of Escherichia coli, was identified as a conformational mimetic of α-melanocyte-stimulating hormone (α-MSH), an anorexigenic neuropeptide. Importantly, ClpB was necessary for E. coli to have an anorexigenic effect in mice, suggesting that it may participate in satiety signaling. To explore this further, we determined the short-term (2 h) effects of three macronutrients: protein (bovine serum albumin), carbohydrate (D-fructose) and fat (oleic acid), on the production of ClpB by E. coli and analyzed whether ClpB can stimulate the secretion of the intestinal satiety hormone, peptide YY (PYY). Isocaloric amounts of all three macronutrients added to a continuous culture of E. coli increased ClpB immunoreactivity. However, to increase the levels of ClpB mRNA and ClpB protein in bacteria and supernatants, supplementation with protein was required. A nanomolar concentration of recombinant E. coli ClpB dose-dependently stimulated PYY secretion from the primary cell cultures of rat intestinal mucosa. Total proteins extracted from E. coli but not from ClpB-deficient E. coli strains also tended to increase PYY secretion. These data support a possible link between E. coli ClpB and protein-induced satiety signaling in the gut.
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Keywords:
gut microbiota; satiety hormones; macronutrients; E. coli; ClpB; PYY
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MDPI and ACS Style
Dominique, M.; Breton, J.; Guérin, C.; Bole-Feysot, C.; Lambert, G.; Déchelotte, P.; Fetissov, S. Effects of Macronutrients on the In Vitro Production of ClpB, a Bacterial Mimetic Protein of α-MSH and Its Possible Role in Satiety Signaling. Nutrients 2019, 11, 2115.
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