Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration

Fahey, Jed W.; Wade, Kristina L.; Stephenson, Katherine K.; Panjwani, Anita A.; Liu, Hua; Cornblatt, Grace; Cornblatt, Brian S.; Ownby, Stacy L.; Fuchs, Edward; Holtzclaw, Walter David; Cheskin, Lawrence J.

doi:10.3390/nu11071489

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Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration

Jed W. Fahey

^1,2,3,4,*

Kristina L. Wade

^1,3,

Katherine K. Stephenson

^1,3,

^1,4

^1,3

⁵,

⁵,

⁵,

²,

Walter David Holtzclaw

^1,3 and

Lawrence J. Cheskin

^6,†

Cullman Chemoprotection Center, Johns Hopkins University, Baltimore, MD 21205, USA

Division of Clinical Pharmacology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

⁴

Center for Human Nutrition, Department of International Health, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA

⁵

Nutramax Laboratories Consumer Care, Inc., Edgewood, MD 21040, USA

⁶

Department of Health Behavior and Society, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205 USA

Author to whom correspondence should be addressed.

^†

Current address: Department of Nutrition and Food Studies, George Mason University, Fairfax, VA 220030, USA.

Nutrients 2019, 11(7), 1489; https://doi.org/10.3390/nu11071489

Received: 18 May 2019 / Revised: 21 June 2019 / Accepted: 24 June 2019 / Published: 29 June 2019

(This article belongs to the Special Issue Plant Food, Nutrition and Human Health)

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Abstract

We examined whether gastric acidity would affect the activity of myrosinase, co-delivered with glucoraphanin (GR), to convert GR to sulforaphane (SF). A broccoli seed and sprout extract (BSE) rich in GR and active myrosinase was delivered before and after participants began taking the anti-acid omeprazole, a potent proton pump inhibitor. Gastric acidity appears to attenuate GR bioavailability, as evidenced by more SF and its metabolites being excreted after participants started taking omeprazole. Enteric coating enhanced conversion of GR to SF, perhaps by sparing myrosinase from the acidity of the stomach. There were negligible effects of age, sex, ethnicity, BMI, vegetable consumption, and bowel movement frequency and quality. Greater body mass correlated with reduced conversion efficiency. Changes in the expression of 20 genes in peripheral blood mononuclear cells were evaluated as possible pharmacodynamic indicators. When grouped by their primary functions based on a priori knowledge, expression of genes associated with inflammation decreased non-significantly, and those genes associated with cytoprotection, detoxification and antioxidant functions increased significantly with bioavailability. Using principal components analysis, component loadings of the changes in gene expression confirmed these groupings in a sensitivity analysis. View Full-Text

Keywords: chemoprevention; crucifer; nutritional supplement; pharmacokinetics; pharmacodynamics

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MDPI and ACS Style

Fahey, J.W.; Wade, K.L.; Stephenson, K.K.; Panjwani, A.A.; Liu, H.; Cornblatt, G.; Cornblatt, B.S.; Ownby, S.L.; Fuchs, E.; Holtzclaw, W.D.; Cheskin, L.J. Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration. Nutrients 2019, 11, 1489. https://doi.org/10.3390/nu11071489

AMA Style

Fahey JW, Wade KL, Stephenson KK, Panjwani AA, Liu H, Cornblatt G, Cornblatt BS, Ownby SL, Fuchs E, Holtzclaw WD, Cheskin LJ. Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration. Nutrients. 2019; 11(7):1489. https://doi.org/10.3390/nu11071489

Chicago/Turabian Style

Fahey, Jed W., Kristina L. Wade, Katherine K. Stephenson, Anita A. Panjwani, Hua Liu, Grace Cornblatt, Brian S. Cornblatt, Stacy L. Ownby, Edward Fuchs, Walter D. Holtzclaw, and Lawrence J. Cheskin 2019. "Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration" Nutrients 11, no. 7: 1489. https://doi.org/10.3390/nu11071489

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Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration

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