Next Article in Journal
Preconception Health Attitudes and Behaviours of Women: A Qualitative Investigation
Next Article in Special Issue
A Strategy to Deliver Precise Oral Doses of the Glucosinolates or Isothiocyanates from Moringa oleifera Leaves for Use in Clinical Studies
Previous Article in Journal
Mixed Nut Consumption May Improve Cardiovascular Disease Risk Factors in Overweight and Obese Adults
Previous Article in Special Issue
Systematic Review on Polyphenol Intake and Health Outcomes: Is there Sufficient Evidence to Define a Health-Promoting Polyphenol-Rich Dietary Pattern?
Open AccessArticle

Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration

1
Cullman Chemoprotection Center, Johns Hopkins University, Baltimore, MD 21205, USA
2
Division of Clinical Pharmacology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
3
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
4
Center for Human Nutrition, Department of International Health, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA
5
Nutramax Laboratories Consumer Care, Inc., Edgewood, MD 21040, USA
6
Department of Health Behavior and Society, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205 USA
*
Author to whom correspondence should be addressed.
Current address: Department of Nutrition and Food Studies, George Mason University, Fairfax, VA 220030, USA.
Nutrients 2019, 11(7), 1489; https://doi.org/10.3390/nu11071489
Received: 18 May 2019 / Revised: 21 June 2019 / Accepted: 24 June 2019 / Published: 29 June 2019
(This article belongs to the Special Issue Plant Food, Nutrition and Human Health)
We examined whether gastric acidity would affect the activity of myrosinase, co-delivered with glucoraphanin (GR), to convert GR to sulforaphane (SF). A broccoli seed and sprout extract (BSE) rich in GR and active myrosinase was delivered before and after participants began taking the anti-acid omeprazole, a potent proton pump inhibitor. Gastric acidity appears to attenuate GR bioavailability, as evidenced by more SF and its metabolites being excreted after participants started taking omeprazole. Enteric coating enhanced conversion of GR to SF, perhaps by sparing myrosinase from the acidity of the stomach. There were negligible effects of age, sex, ethnicity, BMI, vegetable consumption, and bowel movement frequency and quality. Greater body mass correlated with reduced conversion efficiency. Changes in the expression of 20 genes in peripheral blood mononuclear cells were evaluated as possible pharmacodynamic indicators. When grouped by their primary functions based on a priori knowledge, expression of genes associated with inflammation decreased non-significantly, and those genes associated with cytoprotection, detoxification and antioxidant functions increased significantly with bioavailability. Using principal components analysis, component loadings of the changes in gene expression confirmed these groupings in a sensitivity analysis. View Full-Text
Keywords: chemoprevention; crucifer; nutritional supplement; pharmacokinetics; pharmacodynamics chemoprevention; crucifer; nutritional supplement; pharmacokinetics; pharmacodynamics
Show Figures

Graphical abstract

MDPI and ACS Style

Fahey, J.W.; Wade, K.L.; Stephenson, K.K.; Panjwani, A.A.; Liu, H.; Cornblatt, G.; Cornblatt, B.S.; Ownby, S.L.; Fuchs, E.; Holtzclaw, W.D.; Cheskin, L.J. Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration. Nutrients 2019, 11, 1489. https://doi.org/10.3390/nu11071489

AMA Style

Fahey JW, Wade KL, Stephenson KK, Panjwani AA, Liu H, Cornblatt G, Cornblatt BS, Ownby SL, Fuchs E, Holtzclaw WD, Cheskin LJ. Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration. Nutrients. 2019; 11(7):1489. https://doi.org/10.3390/nu11071489

Chicago/Turabian Style

Fahey, Jed W.; Wade, Kristina L.; Stephenson, Katherine K.; Panjwani, Anita A.; Liu, Hua; Cornblatt, Grace; Cornblatt, Brian S.; Ownby, Stacy L.; Fuchs, Edward; Holtzclaw, Walter D.; Cheskin, Lawrence J. 2019. "Bioavailability of Sulforaphane Following Ingestion of Glucoraphanin-Rich Broccoli Sprout and Seed Extracts with Active Myrosinase: A Pilot Study of the Effects of Proton Pump Inhibitor Administration" Nutrients 11, no. 7: 1489. https://doi.org/10.3390/nu11071489

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop