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Nutrients
Volume 11
Issue 6
10.3390/nu11061351
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Article

Preparation and Identification of Novel Antihypertensive Peptides from the In Vitro Gastrointestinal Digestion of Marine Cobia Skin Hydrolysates

by
Yu-Hsin Lin
1,
Chun-An Chen
2,
Jenn-Shou Tsai
2 and
Guan-Wen Chen
2,*
1
Department of Food Technology and Marketing, Taipei University of Marine Technology, No. 212, Section 9, Yan Ping North Road, Taipei 111, Taiwan
2
Department of Food Science, National Taiwan Ocean University, No. 2 Pei-Ning Road, Keelung 202, Taiwan
*
Author to whom correspondence should be addressed.
Nutrients 2019, 11(6), 1351; https://doi.org/10.3390/nu11061351
Received: 4 May 2019 / Revised: 3 June 2019 / Accepted: 13 June 2019 / Published: 15 June 2019
(This article belongs to the Special Issue Effect of Bioactive Peptides on Human Health)
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Abstract

This research focuses on cobia skin hydrolysates and their antihypertensive effects via the inhibitory activities of angiotensin I-converting enzyme (ACE). Marine fish Cobia (Rachycentron canadum) skin was hydrolysed for 5 h using Protamex and Protease N to obtain the cobia skin protein hydrolysates PX-5 and PN-5, respectively. The soluble protein and peptide contents of the PX-5 were 612 and 270 mg/g, respectively, and for the PN-5, 531 and 400 mg/g, respectively. The IC50 of PX-5 and PN-5 on ACE was 0.221 and 0.291 mg/mL, respectively. Increasing the IC50 from 0.221 to 0.044 mg/mL by simulated gastrointestinal digestion (PX-5G) reduced the ACE-inhibitory capacity of PX-5. Using gel filtration chromatography, the PX-5G was fractioned into eight fractions. The molecular weight of the fifth fraction from PX-5G was between 630 and 450 Da, and the highest inhibitory efficiency ratio on ACE was 1552.4%/mg/mL. We identified four peptide sequences: Trp-Ala-Ala, Ala-Trp-Trp, Ile-Trp-Trp, and Trp-Leu, with IC50 values for ACE of 118.50, 9.40, 0.51, and 26.80 μM, respectively. At a dose of 600 mg PX-5 powder/kg body weight, in spontaneously hypertensive rats PX-5’s antihypertensive effect significantly reduced systolic and diastolic blood pressure by 21.9 and 15.5 mm Hg, respectively, after 4 h of oral gavage. View Full-Text
Keywords: marine fish cobia; gastrointestinal digestion; inhibitory efficiency ratio; systolic blood pressure marine fish cobia; gastrointestinal digestion; inhibitory efficiency ratio; systolic blood pressure
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MDPI and ACS Style

Lin, Y.-H.; Chen, C.-A.; Tsai, J.-S.; Chen, G.-W. Preparation and Identification of Novel Antihypertensive Peptides from the In Vitro Gastrointestinal Digestion of Marine Cobia Skin Hydrolysates. Nutrients 2019, 11, 1351. https://doi.org/10.3390/nu11061351

AMA Style

Lin Y-H, Chen C-A, Tsai J-S, Chen G-W. Preparation and Identification of Novel Antihypertensive Peptides from the In Vitro Gastrointestinal Digestion of Marine Cobia Skin Hydrolysates. Nutrients. 2019; 11(6):1351. https://doi.org/10.3390/nu11061351

Chicago/Turabian Style

Lin, Yu-Hsin, Chun-An Chen, Jenn-Shou Tsai, and Guan-Wen Chen. 2019. "Preparation and Identification of Novel Antihypertensive Peptides from the In Vitro Gastrointestinal Digestion of Marine Cobia Skin Hydrolysates" Nutrients 11, no. 6: 1351. https://doi.org/10.3390/nu11061351

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