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Open AccessArticle

Polyphenol-Enriched Plum Extract Enhances Myotubule Formation and Anabolism while Attenuating Colon Cancer-induced Cellular Damage in C2C12 Cells

Food Chemistry and Nutrition Science Research Laboratory, Agricultural Research Station, College of Agriculture, Petersburg, VA 23806, USA
Department of Biology, College of Natural and Health Sciences, Virginia State University, Petersburg, VA 23806, USA
Abbott-Nutrition Division, Research and Development, 3300 Stelzer Road, Columbus, OH 43219, USA
Valley Children’s Hospital, Madera, CA 93636, USA
Author to whom correspondence should be addressed.
Nutrients 2019, 11(5), 1077;
Received: 5 April 2019 / Revised: 8 May 2019 / Accepted: 11 May 2019 / Published: 15 May 2019
(This article belongs to the Special Issue Nutraceutical, Nutrition Supplements and Human Health)
Preventing muscle wasting in certain chronic diseases including cancer is an ongoing challenge. Studies have shown that polyphenols derived from fruits and vegetables shows promise in reducing muscle loss in cellular and animal models of muscle wasting. We hypothesized that polyphenols derived from plums (Prunus domestica) could have anabolic and anti-catabolic benefits on skeletal muscle. The effects of a polyphenol-enriched plum extract (PE60) were evaluated in vitro on C2C12 and Colon-26 cancer cells. Data were analyzed using a one-way ANOVA and we found that treatment of myocytes with plum extract increased the cell size by ~3-fold (p < 0.05) and stimulated myoblast differentiation by ~2-fold (p < 0.05). Plum extract induced total protein synthesis by ~50% (p < 0.05), reduced serum deprivation-induced total protein degradation by ~30% (p < 0.05), and increased expression of Insulin-Like Growth Factor-1 (IGF-1) by ~2-fold (p < 0.05). Plum extract also reduced tumor necrosis factor α (TNFα)-induced nuclear factor κB (NFκB) activation by 80% (p < 0.05) in A549/NF-κB-luc cells. In addition, plum extract inhibited the growth of Colon-26 cancer cells, and attenuated cytotoxicity in C2C12 myoblasts induced by soluble factors released from Colon-26 cells. In conclusion, our data suggests that plum extract may have pluripotent health benefits on muscle, due to its demonstrated ability to promote myogenesis, stimulate muscle protein synthesis, and inhibit protein degradation. It also appears to protect muscle cell from tumor-induced cytotoxicity. View Full-Text
Keywords: cachexia; plum; cancer; muscle wasting; myoblasts; protein synthesis cachexia; plum; cancer; muscle wasting; myoblasts; protein synthesis
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Alsolmei, F.A.; Li, H.; Pereira, S.L.; Krishnan, P.; Johns, P.W.; Siddiqui, R.A. Polyphenol-Enriched Plum Extract Enhances Myotubule Formation and Anabolism while Attenuating Colon Cancer-induced Cellular Damage in C2C12 Cells. Nutrients 2019, 11, 1077.

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