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Impact of Nutritional Changes on Nonalcoholic Fatty Liver Disease
Open AccessArticle

Fatty Acid Desaturase Involvement in Non-Alcoholic Fatty Liver Disease Rat Models: Oxidative Stress Versus Metalloproteinases

1
Department of Internal Medicine and Therapeutics, University of Pavia, 27100 Pavia, Italy
2
Centro Grandi Strumenti, University of Pavia, 27100 Pavia, Italy
3
Institute of Molecular Genetics, Italian National Research Council (CNR), 27100 Pavia, Italy
4
Department of Molecular Medicine, University of Pavia, 27100 Pavia, Italy
5
Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy
6
Emergency Department, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy
*
Authors to whom correspondence should be addressed.
Both authors contributed equally.
Nutrients 2019, 11(4), 799; https://doi.org/10.3390/nu11040799
Received: 6 March 2019 / Revised: 2 April 2019 / Accepted: 3 April 2019 / Published: 8 April 2019
(This article belongs to the Special Issue Liver, Oxidative Stress and Metabolic Syndromes)
We investigated changes in fatty acid desaturases, D5D, D6D, D9-16D and D9-18D, and their relationship with oxidative stress, matrix metalloproteinases (MMPs) and serum TNF-alpha in two rat models of non-alcoholic fatty liver disease NAFLD. Eight-week-old male Wistar rats fed for 3 weeks with methionine-choline–deficient (MCD) diet and eleven-week-old Obese male Zucker rats were used. Serum levels of hepatic enzymes and TNF-alpha were quantified. Hepatic oxidative stress (ROS, TBARS and GSH content) and MMP-2 and MMP-9 (protein expression and activity) were evaluated. Liver fatty acid profiling, performed by GC-MS, was used for the quantification of desaturase activities. Higher D5D and D9-16D were found in Obese Zucker rats as well as an increase in D9-18D in MCD rats. D6D was found only in MCD rats. A negative correlation between D5D and D9-16D versus TBARS, ROS and TNF-alpha and a positive correlation with GSH were shown in fatty livers besides a positive correlation between D9-18D versus TBARS, ROS and TNF-alpha and a negative correlation with GSH. A positive correlation between D5D or D9-16D or D9-18D versus protein expression and the activity of MMP-2 were found. NAFLD animal models showed comparable serum enzymes. These results reinforce and extend findings on the identification of therapeutic targets able to counteract NAFLD disorder. View Full-Text
Keywords: desaturase; fatty liver; oxidative stress; metalloproteinases; TNF-alpha desaturase; fatty liver; oxidative stress; metalloproteinases; TNF-alpha
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MDPI and ACS Style

Palladini, G.; Di Pasqua, L.G.; Berardo, C.; Siciliano, V.; Richelmi, P.; Mannucci, B.; Croce, A.C.; Rizzo, V.; Perlini, S.; Vairetti, M.; Ferrigno, A. Fatty Acid Desaturase Involvement in Non-Alcoholic Fatty Liver Disease Rat Models: Oxidative Stress Versus Metalloproteinases. Nutrients 2019, 11, 799.

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