Resistant Starch Attenuates Bone Loss in Ovariectomised Mice by Regulating the Intestinal Microbiota and Bone-Marrow Inflammation
by
Yuko Tousen 1, Yu Matsumoto 1, Yuya Nagahata 2, Isao Kobayashi 2, Masahiro Inoue 2 and Yoshiko Ishimi 1,*
Yuko Tousen 1, Yu Matsumoto 1, Yuya Nagahata 2, Isao Kobayashi 2, Masahiro Inoue 2 and Yoshiko Ishimi 1,*
1
Department of Food Function and Labeling, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan
2
Product Development Laboratory, J-OIL MILLs Inc., 11 Kagetoricho, Totsuka-ku, Yokohama, Kanagawa 245-0064, Japan
*
Author to whom correspondence should be addressed.
Nutrients 2019, 11(2), 297; https://doi.org/10.3390/nu11020297
Received: 13 December 2018 / Revised: 20 January 2019 / Accepted: 24 January 2019 / Published: 30 January 2019
(This article belongs to the Special Issue Nutritional Status and Bone Health)
The intestinal microbiota may regulate bone metabolism by reducing levels of pro-inflammatory cytokines, and T cells in bone tissues of oestrogen-deficient mice have been reported. Resistant starch (RS) is a type of dietary fibre and results in changes in the composition of the gut microbiota. We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice. OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet. HAS and AH-HAS diets contained 6.8% and 12% of RS, respectively. After 6 weeks, treatment with HAS or AH-HAS increased the abundance of Bifidobacterium spp. in faeces. The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice. AH-HAS treatment attenuated ovariectomy-induced bone loss. These findings suggest that AH-HAS might change the microbiota and immune status of the bone marrow, resulting in attenuated bone resorption in OVX mice.
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Keywords:
resistant starch; bone; osteoporosis; intestinal microbiota; inflammation
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MDPI and ACS Style
Tousen, Y.; Matsumoto, Y.; Nagahata, Y.; Kobayashi, I.; Inoue, M.; Ishimi, Y. Resistant Starch Attenuates Bone Loss in Ovariectomised Mice by Regulating the Intestinal Microbiota and Bone-Marrow Inflammation. Nutrients 2019, 11, 297.
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