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The Extra-Virgin Olive Oil Polyphenols Oleocanthal and Oleacein Counteract Inflammation-Related Gene and miRNA Expression in Adipocytes by Attenuating NF-κB Activation

1
Department of Pharmacy, University of Pisa, 56126 Pisa, Italy
2
Interdepartmental Research Center “Nutraceuticals and Food for Health” University of Pisa, 56124 Pisa, Italy
3
National Research Council (CNR) Institute of Clinical Physiology (IFC), 73100 Lecce, Italy
4
Doctoral School in Life Sciences, University of Siena, 53100 Siena, Italy
5
Molecular Medicine for Neurodegenerative and Neuromuscular Diseases Unit, IRCCS Fondazione Stella Maris, Calambrone, 56128 Pisa, Italy
6
Division of Pediatric Endocrinology, Diabetes and Obesity, Department of Pediatrics and Adolescent Medicine, University of Ulm, 89075 Ulm, Germany
7
Cardiology Division, Pisa University Hospital, 56126 Pisa, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Nutrients 2019, 11(12), 2855; https://doi.org/10.3390/nu11122855
Received: 23 October 2019 / Revised: 13 November 2019 / Accepted: 19 November 2019 / Published: 21 November 2019
(This article belongs to the Special Issue Olive Oil and Human Health)
Inflammation of the adipose tissue plays an important role in the development of several chronic diseases associated with obesity. Polyphenols of extra virgin olive oil (EVOO), such as the secoiridoids oleocanthal (OC) and oleacein (OA), have many nutraceutical proprieties. However, their roles in obesity-associated adipocyte inflammation, the NF-κB pathway and related sub-networks have not been fully elucidated. Here, we investigated impact of OC and OA on the activation of NF-κB and the expression of molecules associated with inflammatory and dysmetabolic responses. To this aim, fully differentiated Simpson-Golabi-Behmel syndrome (SGBS) adipocytes were pre-treated with OC or OA before stimulation with TNF-α. EVOO polyphenols significantly reduced the expression of genes implicated in adipocyte inflammation (IL-1β, COX-2), angiogenesis (VEGF/KDR, MMP-2), oxidative stress (NADPH oxidase), antioxidant enzymes (SOD and GPX), leukocytes chemotaxis and infiltration (MCP-1, CXCL-10, MCS-F), and improved the expression of the anti-inflammatory/metabolic effector PPARγ. Accordingly, miR-155-5p, miR-34a-5p and let-7c-5p, tightly connected with the NF-κB pathway, were deregulated by TNF-α in both cells and exosomes. The miRNA modulation and NF-κB activation by TNF-α was significantly counteracted by EVOO polyphenols. Computational studies suggested a potential direct interaction between OC and NF-κB at the basis of its activity. This study demonstrates that OC and OA counteract adipocyte inflammation attenuating NF-κB activation. Therefore, these compounds could be novel dietary tools for the prevention of inflammatory diseases associated with obesity. View Full-Text
Keywords: adipocyte; exosome; extra-virgin olive oil; gene expression; inflammation; obesity; oleocanthal; oleacein; miRNA; NF-κB adipocyte; exosome; extra-virgin olive oil; gene expression; inflammation; obesity; oleocanthal; oleacein; miRNA; NF-κB
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MDPI and ACS Style

Carpi, S.; Scoditti, E.; Massaro, M.; Polini, B.; Manera, C.; Digiacomo, M.; Esposito Salsano, J.; Poli, G.; Tuccinardi, T.; Doccini, S.; Santorelli, F.M.; Carluccio, M.A.; Macchia, M.; Wabitsch, M.; De Caterina, R.; Nieri, P. The Extra-Virgin Olive Oil Polyphenols Oleocanthal and Oleacein Counteract Inflammation-Related Gene and miRNA Expression in Adipocytes by Attenuating NF-κB Activation. Nutrients 2019, 11, 2855. https://doi.org/10.3390/nu11122855

AMA Style

Carpi S, Scoditti E, Massaro M, Polini B, Manera C, Digiacomo M, Esposito Salsano J, Poli G, Tuccinardi T, Doccini S, Santorelli FM, Carluccio MA, Macchia M, Wabitsch M, De Caterina R, Nieri P. The Extra-Virgin Olive Oil Polyphenols Oleocanthal and Oleacein Counteract Inflammation-Related Gene and miRNA Expression in Adipocytes by Attenuating NF-κB Activation. Nutrients. 2019; 11(12):2855. https://doi.org/10.3390/nu11122855

Chicago/Turabian Style

Carpi, Sara, Egeria Scoditti, Marika Massaro, Beatrice Polini, Clementina Manera, Maria Digiacomo, Jasmine Esposito Salsano, Giulio Poli, Tiziano Tuccinardi, Stefano Doccini, Filippo M. Santorelli, Maria A. Carluccio, Marco Macchia, Martin Wabitsch, Raffaele De Caterina, and Paola Nieri. 2019. "The Extra-Virgin Olive Oil Polyphenols Oleocanthal and Oleacein Counteract Inflammation-Related Gene and miRNA Expression in Adipocytes by Attenuating NF-κB Activation" Nutrients 11, no. 12: 2855. https://doi.org/10.3390/nu11122855

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