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Article

Hydroxytyrosol Modulates Adipocyte Gene and miRNA Expression Under Inflammatory Condition

1
National Research Council (CNR) Institute of Clinical Physiology (IFC), 73100 Lecce, Italy
2
Laboratory of Molecular Pharmacology, Department of Pharmacy, University of Pisa, 56126 Pisa, Italy
3
Laboratory of Applied Physiology, Department of Biological and Environmental Science and Technology (DISTEBA), University of Salento, 73100 Lecce, Italy
4
Division of Pediatric Endocrinology, Diabetes and Obesity, Department of Pediatrics and Adolescent Medicine, University of Ulm, 89075 Ulm, Germany
5
Cardiology Division, Pisa University Hospital, 56126 Pisa, Italy
*
Author to whom correspondence should be addressed.
Nutrients 2019, 11(10), 2493; https://doi.org/10.3390/nu11102493
Received: 21 September 2019 / Revised: 10 October 2019 / Accepted: 15 October 2019 / Published: 17 October 2019
(This article belongs to the Special Issue Olive Oil and Human Health)
Chronic inflammation of the adipose tissue (AT) is a major contributor to obesity-associated cardiometabolic complications. The olive oil polyphenol hydroxytyrosol (HT) contributes to Mediterranean diet cardiometabolic benefits through mechanisms still partially unknown. We investigated HT (1 and 10 μmol/L) effects on gene expression (mRNA and microRNA) related to inflammation induced by 10 ng/mL tumor necrosis factor (TNF)-α in human Simpson–Golabi–Behmel Syndrome (SGBS) adipocytes. At real-time PCR, HT significantly inhibited TNF-α-induced mRNA levels, of monocyte chemoattractant protein-1, C-X-C Motif Ligand-10, interleukin (IL)-1β, IL-6, vascular endothelial growth factor, plasminogen activator inhibitor-1, cyclooxygenase-2, macrophage colony-stimulating factor, matrix metalloproteinase-2, Cu/Zn superoxide dismutase-1, and glutathione peroxidase, as well as surface expression of intercellular adhesion molecule-1, and reverted the TNF-α-mediated inhibition of endothelial nitric oxide synthase, peroxisome proliferator-activated receptor coactivator-1α, and glucose transporter-4. We found similar effects in adipocytes stimulated by macrophage-conditioned media. Accordingly, HT significantly counteracted miR-155-5p, miR-34a-5p, and let-7c-5p expression in both cells and exosomes, and prevented NF-κB activation and production of reactive oxygen species. HT can therefore modulate adipocyte gene expression profile through mechanisms involving a reduction of oxidative stress and NF-κB inhibition. By such mechanisms, HT may blunt macrophage recruitment and improve AT inflammation, preventing the deregulation of pathways involved in obesity-related diseases. View Full-Text
Keywords: adipocyte; exosome; gene expression; hydroxytyrosol; inflammation; insulin resistance; extra virgin olive oil; miRNA; obesity; polyphenol adipocyte; exosome; gene expression; hydroxytyrosol; inflammation; insulin resistance; extra virgin olive oil; miRNA; obesity; polyphenol
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MDPI and ACS Style

Scoditti, E.; Carpi, S.; Massaro, M.; Pellegrino, M.; Polini, B.; Carluccio, M.A.; Wabitsch, M.; Verri, T.; Nieri, P.; De Caterina, R. Hydroxytyrosol Modulates Adipocyte Gene and miRNA Expression Under Inflammatory Condition. Nutrients 2019, 11, 2493. https://doi.org/10.3390/nu11102493

AMA Style

Scoditti E, Carpi S, Massaro M, Pellegrino M, Polini B, Carluccio MA, Wabitsch M, Verri T, Nieri P, De Caterina R. Hydroxytyrosol Modulates Adipocyte Gene and miRNA Expression Under Inflammatory Condition. Nutrients. 2019; 11(10):2493. https://doi.org/10.3390/nu11102493

Chicago/Turabian Style

Scoditti, Egeria, Sara Carpi, Marika Massaro, Mariangela Pellegrino, Beatrice Polini, Maria A. Carluccio, Martin Wabitsch, Tiziano Verri, Paola Nieri, and Raffaele De Caterina. 2019. "Hydroxytyrosol Modulates Adipocyte Gene and miRNA Expression Under Inflammatory Condition" Nutrients 11, no. 10: 2493. https://doi.org/10.3390/nu11102493

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