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Nutrients 2019, 11(1), 209; https://doi.org/10.3390/nu11010209

Effects of Dietary Protein and Fat Content on Intrahepatocellular and Intramyocellular Lipids during a 6-Day Hypercaloric, High Sucrose Diet: A Randomized Controlled Trial in Normal Weight Healthy Subjects

1
Department of Physiology, University of Lausanne, 1005 Lausanne, Switzerland
2
Department for Biomedical Research, University of Bern and Institute of Diagnostic Interventional and Pediatric Radiology, University Hospital, 3012 Bern, Switzerland
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 14 November 2018 / Revised: 14 January 2019 / Accepted: 15 January 2019 / Published: 21 January 2019
(This article belongs to the Special Issue The Effect of Diet and Nutrition on Postprandial Metabolism)
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Abstract

Sucrose overfeeding increases intrahepatocellular (IHCL) and intramyocellular (IMCL) lipid concentrations in healthy subjects. We hypothesized that these effects would be modulated by diet protein/fat content. Twelve healthy men and women were studied on two occasions in a randomized, cross-over trial. On each occasion, they received a 3-day 12% protein weight maintenance diet (WM) followed by a 6-day hypercaloric high sucrose diet (150% energy requirements). On one occasion the hypercaloric diet contained 5% protein and 25% fat (low protein-high fat, LP-HF), on the other occasion it contained 20% protein and 10% fat (high protein-low fat, HP-LF). IHCL and IMCL concentrations (magnetic resonance spectroscopy) and energy expenditure (indirect calorimetry) were measured after WM, and again after HP-LF/LP-HF. IHCL increased from 25.0 ± 3.6 after WM to 147.1 ± 26.9 mmol/kg wet weight (ww) after LP-HF and from 30.3 ± 7.7 to 57.8 ± 14.8 after HP-LF (two-way ANOVA with interaction: p < 0.001 overfeeding x protein/fat content). IMCL increased from 7.1 ± 0.6 to 8.8 ± 0.7 mmol/kg ww after LP-HF and from 6.2 ± 0.6 to 6.9 ± 0.6 after HP-LF, (p < 0.002). These results indicate that liver and muscle fat deposition is enhanced when sucrose overfeeding is associated with a low protein, high fat diet compared to a high protein, low fat diet. View Full-Text
Keywords: sucrose overfeeding; hepatic steatosis; intramyocellular lipids; intrahepatocellular lipids; dietary protein content; dietary fat content; energy expenditure; plasma triglyceride sucrose overfeeding; hepatic steatosis; intramyocellular lipids; intrahepatocellular lipids; dietary protein content; dietary fat content; energy expenditure; plasma triglyceride
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Surowska, A.; Jegatheesan, P.; Campos, V.; Marques, A.-S.; Egli, L.; Cros, J.; Rosset, R.; Lecoultre, V.; Kreis, R.; Boesch, C.; Pouymayou, B.; Schneiter, P.; Tappy, L. Effects of Dietary Protein and Fat Content on Intrahepatocellular and Intramyocellular Lipids during a 6-Day Hypercaloric, High Sucrose Diet: A Randomized Controlled Trial in Normal Weight Healthy Subjects. Nutrients 2019, 11, 209.

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