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Nutrients 2019, 11(1), 115; https://doi.org/10.3390/nu11010115

Whole Body Protein Oxidation Unaffected after a Protein Restricted Diet in Healthy Young Males

1
Department of Internal Medicine, Division of Nephrology, University of Groningen, University Medical Center Groningen, AA53, PO Box 30.001, 9700 RB Groningen, The Netherlands
2
Research Group Healthy Ageing, Allied Health Care and Nursing, Centre of Expertise Healthy Ageing, Hanze University of Applied Sciences, Petrus Driessenstraat 3, 9714 CA Groningen, The Netherlands
3
Department of Internal Medicine, Division of Nephrology, University of Groningen, University Medical Center Groningen, AA53, PO Box 30.001, 9700 RB Groningen, The Netherlands
4
Department of Rehabilitation and Health Psychology, University of Groningen, University Medical Center Groningen, CD44, PO Box 30.001, 9700 RB Groningen, The Netherlands
5
Department of Cell Biology, University of Groningen, University Medical Center Groningen, FB33, PO Box 30.001, 9700 RB Groningen, The Netherlands
6
Department of Maxillofacial Surgery, University of Groningen, University Medical Center Groningen, BB70, PO Box 30.001, 9700 RB Groningen, The Netherlands
*
Author to whom correspondence should be addressed.
Received: 26 November 2018 / Revised: 14 December 2018 / Accepted: 29 December 2018 / Published: 8 January 2019
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Abstract

Protein oxidation may play a role in the balance between anabolism and catabolism. We assessed the effect of a protein restricted diet on protein oxidation as a possible reflection of whole body protein metabolism. Sixteen healthy males (23 ± 3 years) were instructed to use a 4-day isocaloric protein restricted diet (0.25 g protein/kg body weight/day). Their habitual dietary intake was assessed by a 4-day food diary. After an overnight fast, a 30 g 13C-milk protein test drink was administered, followed by 330 min breath sample collection. Protein oxidation was measured by Isotope Ratio Mass Spectrometry. To assess actual change in protein intake from 24-h urea excretion, 24-h urine was collected. During the 4-day protein restricted diet, the urinary urea:creatinine ratio decreased by 56 ± 9%, which is comparable to a protein intake of ~0.65 g protein/kg body weight/day. After the protein restricted diet, 30.5 ± 7.3% of the 30 g 13C-milk protein was oxidized over 330 min, compared to 31.5 ± 6.4% (NS) after the subject’s habitual diet (1.3 ± 0.3 g protein/kg body weight/day). A large range in the effect of the diet on protein oxidation (−43.2% vs. +44.0%) was observed. The residual standard deviation of the measurements was very small (0.601 ± 0.167). This suggests that in healthy males, protein oxidation is unaffected after a protein restricted diet. It is uncertain how important the role of fluctuations in short-term protein oxidation is within whole body protein metabolism. View Full-Text
Keywords: Protein; oxidation; anabolic competence; breath test; naturally enriched 13C-milk proteins Protein; oxidation; anabolic competence; breath test; naturally enriched 13C-milk proteins
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Reckman, G.A.; Navis, G.J.; Krijnen, W.P.; Van der Schans, C.P.; Vonk, R.J.; Jager-Wittenaar, H. Whole Body Protein Oxidation Unaffected after a Protein Restricted Diet in Healthy Young Males. Nutrients 2019, 11, 115.

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