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Nutrients 2018, 10(6), 691;

Milk Fermented by Specific Lactobacillus Strains Regulates the Serum Levels of IL-6, TNF-α and IL-10 Cytokines in a LPS-Stimulated Murine Model

Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Carretera a La Victoria Km. 0.6, Apartado 1735, Hermosillo, Sonora 83304, Mexico
Author to whom correspondence should be addressed.
Received: 28 April 2018 / Revised: 18 May 2018 / Accepted: 23 May 2018 / Published: 29 May 2018
(This article belongs to the Special Issue Dairy Products)
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Studies report that metabolites, such as peptides, present in fermented milk with specific lactic acid bacteria, may regulate cytokine production and exert an anti-inflammatory effect. Hence, the cytokine regulatory effect of fermented milk by specific Lactobacillus strains was evaluated in a lipopolysaccharide (LPS)-stimulated murine model. From twelve strains, three (J20, J23 and J28) were selected for their high proteolytic and acidifying capacities in milk and used for the in vivo study. Three treatments (fermented milk, FM; pasteurized fermented milk, PFM; and its <10 kDa fractions, PFM10) were administrated daily for four weeks. After treatments, animals were induced to a systemic inflammation with LPS, and blood samples were collected 6 h post-LPS injection for cytokine analyses. Results showed that FM or PFM significantly (p > 0.05) reduced pro-inflammatory cytokine (IL-6 and TNF-α) concentrations and significantly increased anti-inflammatory (IL-10) cytokine concentrations in comparison to the control; also, pro-inflammatory cytokines were reduced for animals treated with PFM10 (p < 0.05). RP-HPLC-MS/MS analysis showed that water-soluble extracts (<10 kDa) from PFM with J28 presented 15 new peptides, which may be the metabolites involved in the cytokine regulatory effect of fermented milk. View Full-Text
Keywords: fermented milk; cytokine regulation; Lactobacillus; lipopolysaccharide fermented milk; cytokine regulation; Lactobacillus; lipopolysaccharide

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Reyes-Díaz, A.; Mata-Haro, V.; Hernández, J.; González-Córdova, A.F.; Hernández-Mendoza, A.; Reyes-Díaz, R.; Torres-Llanez, M.J.; Beltrán-Barrientos, L.M.; Vallejo-Cordoba, B. Milk Fermented by Specific Lactobacillus Strains Regulates the Serum Levels of IL-6, TNF-α and IL-10 Cytokines in a LPS-Stimulated Murine Model. Nutrients 2018, 10, 691.

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