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Peripheral Blood Mononuclear Cell Metabolism Acutely Adapted to Postprandial Transition and Mainly Reflected Metabolic Adipose Tissue Adaptations to a High-Fat Diet in Minipigs

INRA, UNH, Unité de Nutrition Humaine, CRNH Auvergne, Université Clermont Auvergne, F-63000 Clermont-Ferrand, France
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Nutrients 2018, 10(11), 1816; https://doi.org/10.3390/nu10111816
Received: 29 September 2018 / Revised: 26 October 2018 / Accepted: 15 November 2018 / Published: 21 November 2018
(This article belongs to the Special Issue The Effect of Diet and Nutrition on Postprandial Metabolism)
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Abstract

Although peripheral blood mononuclear cells (PBMCs) are widely used as a valuable tool able to provide biomarkers of health and diseases, little is known about PBMC functional (biochemistry-based) metabolism, particularly following short-term nutritional challenges. In the present study, the metabolic capacity of minipig PBMCs to respond to nutritional challenges was explored at the biochemical and molecular levels. The changes observed in enzyme activities following a control test meal revealed that PBMC metabolism is highly reactive to the arrival of nutrients and hormones in the circulation. The consumption, for the first time, of a high fat–high sucrose (HFHS) meal delayed or sharply reduced most of the observed postprandial metabolic features. In a second experiment, minipigs were subjected to two-month HFHS feeding. The time-course follow-up of metabolic changes in PBMCs showed that most of the adaptations to the new diet took place during the first week. By comparing metabolic (biochemical and molecular) PMBC profiles to those of the liver, skeletal muscle, and adipose tissue, we concluded that although PBMCs conserved common features with all of them, their response to the HFHS diet was closely related to that of the adipose tissue. As a whole, our results show that PBMC metabolism, particularly during short-term (postprandial) challenges, could be used to evaluate the whole-body metabolic status of an individual. This could be particularly interesting for early diagnosis of metabolic disease installation, when fasting clinical analyses fail to diagnose the path towards the pathology. View Full-Text
Keywords: peripheral blood mononuclear cells; postprandial metabolism; high fat–high sugar diet; minipig; adipose tissue; biomarkers peripheral blood mononuclear cells; postprandial metabolism; high fat–high sugar diet; minipig; adipose tissue; biomarkers
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Zeng, Y.; David, J.; Rémond, D.; Dardevet, D.; Savary-Auzeloux, I.; Polakof, S. Peripheral Blood Mononuclear Cell Metabolism Acutely Adapted to Postprandial Transition and Mainly Reflected Metabolic Adipose Tissue Adaptations to a High-Fat Diet in Minipigs. Nutrients 2018, 10, 1816.

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