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Open AccessArticle

Maternal Choline and Betaine Supplementation Modifies the Placental Response to Hyperglycemia in Mice and Human Trophoblasts

1
Department of Health and Nutrition Sciences, Brooklyn College of the City University of New York, Brooklyn, NY 11210, USA
2
Department of Biology, Brooklyn College of the City University of New York, Brooklyn, NY 11210, USA
3
Packer Collegiate Institute, Brooklyn, NY 11201, USA
*
Author to whom correspondence should be addressed.
Nutrients 2018, 10(10), 1507; https://doi.org/10.3390/nu10101507
Received: 29 August 2018 / Revised: 4 October 2018 / Accepted: 10 October 2018 / Published: 15 October 2018
(This article belongs to the Special Issue The Health Aspects of Dietary Choline)
Gestational diabetes mellitus (GDM) is characterized by excessive placental fat and glucose transport, resulting in fetal overgrowth. Earlier we demonstrated that maternal choline supplementation normalizes fetal growth in GDM mice at mid-gestation. In this study, we further assess how choline and its oxidation product betaine influence determinants of placental nutrient transport in GDM mice and human trophoblasts. C57BL/6J mice were fed a high-fat (HF) diet 4 weeks prior to and during pregnancy to induce GDM or fed a control normal fat (NF) diet. The HF mice also received 25 mM choline, 85 mM betaine, or control drinking water. We observed that GDM mice had an expanded placental junctional zone with an increased area of glycogen cells, while the thickness of the placental labyrinth zone was decreased at E17.5 compared to NF control mice (p < 0.05). Choline and betaine supplementation alleviated these morphological changes in GDM placentas. In parallel, both choline and betaine supplementation significantly reduced glucose accretion (p < 0.05) in in vitro assays where the human choriocarcinoma BeWo cells were cultured in high (35.5 mM) or normal (5.5 mM) glucose conditions. Expression of angiogenic genes was minimally altered by choline or betaine supplementation in either model. In conclusion, both choline and betaine modified some but not all determinants of placental transport in response to hyperglycemia in mouse and in vitro human cell line models. View Full-Text
Keywords: choline; betaine; gestational diabetes; placental morphology; nutrient transport; vasculature choline; betaine; gestational diabetes; placental morphology; nutrient transport; vasculature
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MDPI and ACS Style

Nanobashvili, K.; Jack-Roberts, C.; Bretter, R.; Jones, N.; Axen, K.; Saxena, A.; Blain, K.; Jiang, X. Maternal Choline and Betaine Supplementation Modifies the Placental Response to Hyperglycemia in Mice and Human Trophoblasts. Nutrients 2018, 10, 1507.

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